EMAGE mouse gene expression spatial database

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EMAGE:14110

C85492 expressed sequence C85492 ( MGI:2143424)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001765. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001765_01	euxassay_001765_02	euxassay_001765_03	euxassay_001765_04

euxassay_001765_05	euxassay_001765_06	euxassay_001765_07	euxassay_001765_08	euxassay_001765_09

euxassay_001765_10	euxassay_001765_11	euxassay_001765_12	euxassay_001765_13	euxassay_001765_14

euxassay_001765_15	euxassay_001765_16	euxassay_001765_17	euxassay_001765_18	euxassay_001765_19

euxassay_001765_20	euxassay_001765_21	euxassay_001765_22	euxassay_001765_23	euxassay_001765_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14110_wholemount_strong.wlz

14110_wholemount_moderate.wlz

14110_wholemount_weak.wlz

14110_wholemount_possible.wlz

14110_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14110_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4390
Entity Detected:	C85492, expressed sequence C85492 ( MGI:2143424)
Sequence:	sense strand is shown >T4390 CGGACGCGTGGGGGAACACCTGCGACACCCTGTCGGCAGGTGGGCAGCGCGGGCATCTGTGGCGCAGCTG GGCCCCGGGCTCGGAGCGGACGGGGCACAGAGCCCGGCTTCAGGCATTTTCTGGTCCTCCAGTAGTACAC TTAGGGATGGATGGGTGAAGTCCCCCGGGCTGTGGCACTGCCCAGCAGCCATCGTCTCGCTGAGGCCCCC TGGTGGCGATAGGATGCACCTCTCTGCCGTATTCAACGCCCTCCTGGTATCAGTGCTGGCTGCGGTCCTG TGGAAGCATGTTCGGTTGCGGGAGCATGCGGCCACGCTAGAGGAGGAGCTGGCCCTTGGACAGCAGTCCC TGGATCCAGTCCTAGGACTGAAGATCGACTACCCGAAGGCCCTGCAGATCCTCATGGAGGGCGGCACCCA CATGGTGTGCACAGGCCGCACCCACACGGACCGCATCTGCCGCTTCAAGTGGCTCTGCTACTCCAATGAG GCCGAAGAATTCATCTTTTTCCATGGCAACTCATCTGTGATGCTGCCCAACCTGGGCTCCCGGCGCTTCC AGCCGGCCCTGCTGGACCTGTCCACTGTGGAGGACCATAATGCCCAGTACTTCAACTTCGTGGAGCTGCC CGCTGCAGCCCTGCGCTTCATGCCAAAGCCAGTGTTCGTGCCTGATGTAGCCCTCATCGCCAACCGCTTC AACCCCGATAACCTCATGCACGTCTTTCATGATGACCTGCTCCCACTCTTCTACACACTGAGGCAGTTCC CCGGCCTGGCCCAAGAGGCCCGGCTCTTTTTCATGGAGGGATGGGGTGAGGGTGCCCACTTTGACCTCTA TAAACTCCTCAGCCCCAAGCAGCCGCTTCTACGTGCACAGCTCAAGACACTGGGCCGACTGCTCTGCTTT TCCCATGCTTTCGTGGGCCTGTCCAAGGTTACCACCTGGTACCAATACGGCTTTGTACAGCCCCAGGGCC CAAAAGCCAACATCCTGGTCTCTGGCAATGAGATCCGGCAGTTCACGCGCTTCATGACTGAAAGGCTGAA CGTGAGCCACGCGGGGGCGCCCCTGGGGGAGGAATACATTCTGGTCTTCAGTCGCACCCAGAACAGACTC ATCCTGAACGAGGCAGAGCTGTTGCTGGAGCTGGCACAAGAGTTCCAGATGAAGACGGTTACAGTGTCCC TGGAGGACCACACCTTTGCTGATGTCGTGCGGCTGGTCAGCAATGCCTCCATGTTGGTCAGCATGCATGG GGCCCAGCTGGTTACGGCCCTCTTCCTGCCCCGCGGGGCTACCGTGGTTGAACTCTTCCCTTACGCTGTC AATCCTGACCACTACACCCCCTATAAGACGCTGGCCACACTGCCTGGCATGGACCTGCAGTATGTAGCCT GGCGAAACATGATCCGGGAGAACACAGTCACACACCCTGAGCGACCGTGGGACCAAGGGGGCATCACCCA CCTGGACCGGGCTGAGCAGGCCCGTATCCTGCAGAGCCGAGAGGTCCCCCGGCATCTCTGTTGCCGGAAC CCAGAGTGGCTATTCCGAATCTACCAGGACACCAGGGTGGACATCCCATCCCTTATGCAATCCATTCGGC GTGTGGTGAAGGGCCGGCCGGGGCCACGGAGGCAGAGGTGGGCAATCAGCCTGTACCCGGGCAAGGTGCG GGAGGCTCGATGTCAGGCGTCAGTGCAGGGTGCCACCGAGGCCCGCCTGTCTGTGTCCTGGCAGATCCCG TGGAACCTCAAGTACCTGAAAGTGAGGGAGGTGAAATACGAGGTGTGGCTGCAGGAGCAAGGGGAGAACA CGTATGTGCCTTACATGCTGACCCTGCAGAACCACACCTTCACAGAGAACATCAAACCTTTTACCACCTA CCTGGTGTGGGTCCGCTGTATATTTAACAGGACCCTCCTGGGACCTTTTGCAGATGTGCTGGTGTGCAGC ACGTAGCCAGCAGACCATGGCCAGGCCTCTGGAGGGCACCTCTCGCAGCTCAGCTTTCCCAGGTCCACAG TTCTGCTAGCAGCTGTGGTGATAAATTATCTATTTATTGAGTAGGCTTGCACTAAGCCTGCTTCCCGAGT GTGCTTTTTGCAGATTTCTCTAGCTCTGAAGAGGTGGTGCCTGCCAGGGCCCGGGGATCTGCTCTTGCTA ATAACCATGCTGTTTCCTGCTTTGGCCTTGGTGTAGGTCCTGGCCTCGAGAAACAGTGGTCACGTGGTTG GTGGGCTAGGCAGATAATCTTTGAATTTCATCTGTAGCTGAGTGACAGGTGTGTTTGGAGCAGTCATTAA ATGATTATGAACATCTTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4914234 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4914234 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14106 same embryo
	EMAGE:14107 same embryo
	EMAGE:14111 same embryo
	EMAGE:14108 same embryo
	EMAGE:14109 same embryo
	EurExpress:euxassay_001765 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source