EMAGE mouse gene expression spatial database

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EMAGE:14125

Chchd3 coiled-coil-helix-coiled-coil-helix domain containing 3 ( MGI:1913325)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001805. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001805_01	euxassay_001805_02	euxassay_001805_03	euxassay_001805_04

euxassay_001805_05	euxassay_001805_06	euxassay_001805_07	euxassay_001805_08	euxassay_001805_09

euxassay_001805_10	euxassay_001805_11	euxassay_001805_12	euxassay_001805_13	euxassay_001805_14

euxassay_001805_15	euxassay_001805_16	euxassay_001805_17	euxassay_001805_18	euxassay_001805_19

euxassay_001805_20	euxassay_001805_21	euxassay_001805_22	euxassay_001805_23	euxassay_001805_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14125_wholemount_strong.wlz

14125_wholemount_moderate.wlz

14125_wholemount_weak.wlz

14125_wholemount_possible.wlz

14125_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14125_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 10 11 13 14

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 24 weak expression: see section 10 11 12 13 14 15 16 17 18 19 20

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4929
Entity Detected:	Chchd3, coiled-coil-helix-coiled-coil-helix domain containing 3 ( MGI:1913325)
Sequence:	sense strand is shown >T4929 GGTCGTGGCCTTGCTCCTGCCCTGCAAGAGAAGCGTCCAGGGCTTTCACGCGCAAGTGTGCGCGTGGGTC CTGACGTGCTCGTAGGTCCACGCTCCAGACTCTGCGGGGACAGAAGCAGGAGCCATGGGCGGGACCGCTA GCACCCGCCGGGTCACCTTCGAGGCGGACGAGAACGAGAACATCACCGTGGTGAAGGGCATCCGGCTTTC AGAAAATGTGATCGACCGGATGAAGGAGTCCTCTCCATCTGGCTCTAAGTCTCAGCGATATTCCAGCGTT TATGGTGCTTCAGTTTCTGATGAAGATTTGAAAAGAAGAGTAGCTGAGGAGCTGGCATTGGAGCAAGCCA AAAAGGAGTCAGAACACCAGAGACGCCTTAAGCAAGCAAGGGACCTAGAAAGAGAAAGGGCTGCAGCCAA TGAGCAGCTGACCAGAGCTGTCCTTCGGGAAAGAATATCCAGTGAAGAGGAGCGCATGAAGGCGAAGCAT CTGGCTCGGCAGCTGGAAGAGAAAGACCGAGTGATGCGGAAGCAGGATGCATTCTACAAAGAGCAGCTGG CCAGACTGGAGGAGAGGAGCTCAGAGTTCTACAAAGTCACCACCGAGGAGTATCAGAAAGCTGCTGAAGA GGTGGAAGCCAAGTTCAAGCGGTATGAATATCATCCAGTCTGTGCAGATCTGCAGACCAAAATCCTCCAG TGTTACCGTCAGAACACCCAGCAGACTCTCAGCTGCTCTGCTCTGGCTAGCCAATACATGCACTGTGTCA ACCATGCCAAACAGAGCATGCTGGAGAAGGGAGGCTAAAGTTACCCTGAGAACATGCAAGCTCCTTCAAC GTTAATTCCAGAGGTGGAACATTTTTCTTTTCCTAGTATGAAAATGACCCATTTAAAGAGAAGACCACTA AAGACAGACAGGCACTGGAAAATGGACTCAACTGAATCATAACGTGTTTTGATCAACAGTTTAAAAAGCC ATGTTCTAGATCAGCGATCAGTCATCAAAGAACACTGTTAAGTGTTAATGAAACCACAAAATGAATAAAA GAGGAAGGCAGTCCACCCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5124504 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5124504 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14126 same embryo
	EMAGE:14129 same embryo
	EMAGE:14130 same embryo
	EMAGE:14128 same embryo
	EMAGE:14127 same embryo
	EurExpress:euxassay_001805 same experiment
	MGI:4823836 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source