EMAGE mouse gene expression spatial database

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EMAGE:14128

Lipc lipase, hepatic ( MGI:96216)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001831. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001831_01	euxassay_001831_02	euxassay_001831_03	euxassay_001831_04

euxassay_001831_05	euxassay_001831_06	euxassay_001831_07	euxassay_001831_08	euxassay_001831_09

euxassay_001831_10	euxassay_001831_11	euxassay_001831_12	euxassay_001831_13	euxassay_001831_14

euxassay_001831_15	euxassay_001831_16	euxassay_001831_17	euxassay_001831_18	euxassay_001831_19

euxassay_001831_20	euxassay_001831_21	euxassay_001831_22	euxassay_001831_23	euxassay_001831_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14128_wholemount_strong.wlz

14128_wholemount_moderate.wlz

14128_wholemount_weak.wlz

14128_wholemount_possible.wlz

14128_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14128_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4928
Entity Detected:	Lipc, lipase, hepatic ( MGI:96216)
Sequence:	sense strand is shown >T4928 AGGAAAGAAGACCTGGCGAGGTGTTTTATTTCCAAAACTTATCGAGATAAAAAGTGCTTCTGTGGGTCAA TCTAGATATTGATAGATTTGGACTGGAGAAGGTAAAGAAAGTGTTTAAATTCTAGGTAACGTGTTTTAAG GTTAATAATTAATGGGGGCCTTCCCCAGCTGAGCACCAAGAAGCACTCTGGTCTCTTTAGTTCTAGACAT TAGCAAGAAGACCTCAGACCCCAGGTGAGGCCAGAGACATGGGAAATCCCCTCCAAATCTCCATTTTCCT GGTGTTCTGCATCTTTATCCAATCAAGTGCTTGTGGACAAGGCGTGGGAACAGAGCCCTTTGGGAGAAGC CTTGGAGCTACTGAAGCTAGCAAGCCATTAAAGAAGCCAGAGACCAGATTCCTGCTCTTCCAAGATGAAA ACGATCGCCTGGGCTGTCGTCTCAGACCTCAGCACCCGGAAACACTGCAGGAGTGTGGCTTCAACAGCTC TCAGCCGCTTATCATGATCATCCACGGGTGGTCGGTGGATGGCTTGCTAGAAAACTGGATCTGGAAGATA GTGAGTGCGCTGAAGTCCCGACAGTCCCAACCTGTGAATGTGGGGTTAGTGGACTGGATCTCCCTGGCAT ACCAGCACTACACCATTGCTGTTCAAAACACCCGTATTGTGGGCCAGGACGTGGCTGCTCTTCTCCTATG GCTGGAGGAATCTGCGAAGTTTTCTCGGAGCAAAGTTCACCTAATTGGGTACAGCCTGGGAGCGCACGTC TCAGGGTTCGCAGGCAGCTCCATGGACGGGAAGAACAAGATTGGAAGAATCACAGGGCTGGACCCTGCGG GCCCTATGTTTGAGGGAACGTCCCCCAACGAGCGCCTTTCTCCTGATGATGCCAATTTTGTGGACGCCAT TCATACCTTTACCAGGGAGCACATGGGCTTGAGTGTGGGCATCAAGCAGCCCATTGCCCACTATGACTTC TACCCCAATGGGGGCTCCTTCCAGCCTGGCTGCCACTTCCTGGAACTCTACAAACACATTGCAGAGCATG GCTTAAACGCCATAACCCAGACCATCAAATGTGCCCATGAGCGCTCCGTGCACCTCTTCATTGACTCCTT GCAACACAGTGACCTGCAGAGCATCGGCTTCCAGTGCAGCGACATGGGCAGCTTCAGCCAAGGTCTATGC CTGAGCTGCAAGAAGGGCCGTTGCAACACTCTGGGTTATGACATCCGCAAGGACCGGTCAGGCAAGAGCA AGAGGCTCTTCCTCATCACGCGAGCCCAGTCTCCCTTCAAAGTTTATCATTACCAGTTCAAGATCCAGTT CATCAATCAAATTGAGAAGCCGGTAGAGCCTACTTTTACCATGTCGCTGCTGGGAACAAAAGAAGAAATA AAGAGAATTCCCATCACCCTGGGCGAAGGAATTACCAGCAATAAAACCTATTCCTTCCTTATCACACTGG ACAAAGACATCGGCGAGTTGATCCTGCTCAAGTTCAAGTGGGAAAACAGTGCAGTGTGGGCCAATGTGTG GAACACAGTGCAGACCATCATGCTATGGGGCATAGAACCTCACCACTCTGGCCTCATTCTGAAGACCATC TGGGTCAAAGCTGGAGAGACGCAGCAAAGAATGACATTTTGCCCCGAAAATCTGGATGACCTCCAGCTTC ACCCGAGCCAGGAGAAAGTCTTTGTGAACTGTGAAGTGAAGTCAAAAAGACTGACTGAATCGAAAGAGCA GATGAGTCAAGAGACCCATGCAAAAAAATAAAGAAGTCTATTCTTTATCTCTAATGGTTGCCTTATTTGG AAGCCAAAATTACACAAAGGATCAATCATGCATCCATTTAAATAAAGTTTGTATGTAAAGGAGGGTGTGC TTTGCTGTTATAAACTAAGCTTTCAAACTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5124139 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5124139 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14126 same embryo
	EMAGE:14125 same embryo
	EMAGE:14129 same embryo
	EMAGE:14130 same embryo
	EMAGE:14127 same embryo
	EurExpress:euxassay_001831 same experiment
	MGI:4825934 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source