EMAGE mouse gene expression spatial database

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EMAGE:14143

Twf1 twinfilin, actin-binding protein, homolog 1 (Drosophila) ( MGI:1100520)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001845. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001845_01	euxassay_001845_02	euxassay_001845_03	euxassay_001845_04

euxassay_001845_05	euxassay_001845_06	euxassay_001845_07	euxassay_001845_08	euxassay_001845_09

euxassay_001845_10	euxassay_001845_11	euxassay_001845_12	euxassay_001845_13	euxassay_001845_14

euxassay_001845_15	euxassay_001845_16	euxassay_001845_17	euxassay_001845_18	euxassay_001845_19

euxassay_001845_20	euxassay_001845_21	euxassay_001845_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14143_wholemount_strong.wlz

14143_wholemount_moderate.wlz

14143_wholemount_weak.wlz

14143_wholemount_possible.wlz

14143_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14143_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4445
Entity Detected:	Twf1, twinfilin, actin-binding protein, homolog 1 (Drosophila) ( MGI:1100520)
Sequence:	sense strand is shown >T4445 GGCCGCTACCGCACCCTGCGCCGGAGCCGGGAGCCTGGGGCCGCCGCCATGTCCCACCAGACCGGCATCC AAGCAAGCGAAGATGTCAAAGAGATCTTTGCCCGAGCCAGAAACGGGAAATACAGACTTCTGAAAATATC TATCGAAAATGAGCAACTGGTGGTTGGCTCCTGTAGTCCACCTTCAGACTCCTGGGAACAGGACTACGAC TCCTTTGTCTTGCCCCTGCTGGAGGACAAGCAGCCGTGCTATGTATTATTCAGGTTAGACTCTCAGAATG CCCAGGGATATGAATGGATATTCATTGCATGGTCTCCAGATCATTCTCATGTACGTCAGAAAATGCTGTA TGCGGCAACAAGAGCGACTCTGAAGAAAGAATTTGGAGGTGGCCACATTAAAGATGAAGTGTTTGGAACA GTAAAGGAGGACGTATCATTACATGGATATAAAAAGTATTTGCTGTCCCAGTCTTCCCCTGCCCCGCTGA CCGCCGCTGAGGAAGAATTACGGCAGATTAAAATCAACGAGGTCCAAACTGATGTGAGTGTGGACACAAA GCACCAGACACTCCAAGGCGTAGCCTTTCCTATTTCTCGAGATGCCTTCCAGGCTTTGGAGAAGCTGAGT AAGAAGCAGCTCAACTATGTGCAGTTGGAAATAGATATAAAAAATGAAACAATAATTTTGGCCAACACAG AAAATACAGAACTGAGAGATTTGCCAAAGAGGATTCCCAAGGATTCAGCGCGTTACCATTTCTTTCTGTA CAAGCATTCCCACGAAGGAGACTACTTAGAGTCCGTAGTTTTTATTTATTCGATGCCTGGATACACGTGC AGCATAAGAGAGCGGATGCTGTATTCCAGCTGCAAGAGCCCTCTGCTCGAGATTGTAGAGCGACAGCTAC AGATGGATGTGATCAGAAAGATTGAGATAGACAATGGGGATGAGCTGACTGCGGATTTCCTGTACGATGA AGTCCACCCCAAGCAGCATGCACATAAGCAGAGTTTTGCTAAACCGAAAGGTCCTGCGGGAAAGAGGGGG ATCCGAAGATTGATTAGGGGCCCAGCGGAAGCAGAAGCCACTACTGACTAGAACTGTCGTATTAAATGTT GCAATATAGTTTTGTAAAGTTTCCAGCTTTTAGTACAAGGAGAACTGAAATCATTCCATGTTGATAGAGT TGGGAGGAAAATTGTACTTTTTGAAAAATAGCACTTTTCACTTCTATGTATTTTTTAAATTAATGCTATA GAAGACTCATGATTTTTATTTTTTAGTTAAAACTAGACAAGGTTTCAACATAGTGATGTTTGGTTTTGTC ACACTGTTTTCATAACGTGTTGATTCCACACTTTCACATAATCTTCAATATTTCCCATAGGTGGGCCAGT TCCACCAAGCATAAGGTCTTGCTCTGTAGAACTCTGTAGTGAGGTCTAACGTTCCCTATGAAGGGAAGCG AACGACGAACAACCCTGAGATACTCACACCTGTCGAGTGTGTTAAGGAGCCTGGGAGGAGGCGGGCAGCT GATTGCTTTGAGCATGCTCAGGAAGGGCTGTCTGCCGCGGTGGAGAAAGGCAAACTGCAGCAGGGCTTCC TGTCCCTCCTCTCCATTGTAGTAGCATCAGATCTCAGAAACGAAGTTCACACTGAAGCTCGAAAGGTACC AGACAGTGCAGGCTTCCCATCCCATCCCCCGCCCCCCTGCACTGGAAGACTCGCGTCCAGTAAGGTCTTT GCAGAACTTTCGAGCTATCTATTTCCAAAGTGAGTCCAGAGGGCTGACGCCAGACGTTCATGTTCGAAAG CCAAGGATCGCTCAAAAAAAAACAAAACTAAAGAAAAATGTTGGAAGTTTCAAGGGGTTTTTTTTTTTTT TTTTTTTGGTTTTGTGTTTTGTTTTTGTTTTTGTTTTTCTCTTTGAAACCATGTATCTTAAAGCAAATTG TCCTGTTGCTTGGGAGCAAGTGTTCAGATCTAAAATTATTGATTTCTACTACTTAGTAGGCTGACACAGA TAATACTTAGCCCAGGGATCTTGTTTCAGTGAATAATGTCTCAGAACACGGGTTGTCTGAAGCTTTGTGT TTTCAGTTACAACCTAGAGAGTTTAGAGCCTCAAGAGTTCTTTGTTACTTGAAATAGAGGGAGTTAGAAC ACTTGGTGATTTCATCTGCTAAACCTGCTGCAGAGCCATAACTGAGAAGTTCTGAAGTGGACCATCGTGT CCAGGACCTGGAAGTCCTCGACCTGAACTTCATGCTGCACAAGATAATGTCAGTAATGTACATTTCATGG TATGAAATATGAAGCACCCATTGCAGAGCCTTATTATCTAATACACACGCCTTTCAGAAATTCAGCGTGT ATTCACATAGACCATAAGAGGAATTACAGGCTGATGGAAATTTTTTAAAATAGATTTGATTATTGTGTGT TTTGTTTGTTTACAAATACGTAGACTTTACTACTTACATATGCAGGACAGTCGACAGTGACGTCACACTT GTTTACGAGAGGTAAACTTTTTAGTTGTACGTGTGTACAGTTGTCACACGTGCCATAGAGTGCTGCACTG AGAGTCCTGGAGCCCTGTCTAGACCATCACCACAGCTGCCACTTCAAGCAGAGGGAGCACTACGTGTCCT GGATCCAGAGCCAGTGGGGGACTGTTCTGAGGCATCACGTGCAACGATGCTCTGAAGCCTAGTACACTAC ATCGAAGTCAATGCACATTCCAGAATATAGATGTGATCCTGTAACCCTACTAACGATTAAACCAATGATT GCTGAGAATCACGTATGCATTTGTTTTAGGGTATAACTCATTGTTTACACTATGTTTTAGGTGACAATAG ATGTGAATAACATCCCCAATAAATTTATATAGCAGTGAAAAATTACATGTCTTCTGTGGACATTTTATAA GTGCATTTTATATCAATAAAATTTTTCTCTTTGAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4934593 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4934593 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14145 same embryo
	EMAGE:14144 same embryo
	EMAGE:14142 same embryo
	EMAGE:14146 same embryo
	EurExpress:euxassay_001845 same experiment
	MGI:4829025 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source