EMAGE mouse gene expression spatial database

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EMAGE:14170

1810031K17Rik RIKEN cDNA 1810031K17 gene ( MGI:1916421)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003947. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003947_01	euxassay_003947_02	euxassay_003947_03	euxassay_003947_04

euxassay_003947_05	euxassay_003947_06	euxassay_003947_07	euxassay_003947_08	euxassay_003947_09

euxassay_003947_10	euxassay_003947_11	euxassay_003947_12	euxassay_003947_13	euxassay_003947_14

euxassay_003947_15	euxassay_003947_16	euxassay_003947_17	euxassay_003947_18	euxassay_003947_19

euxassay_003947_20	euxassay_003947_21	euxassay_003947_22	euxassay_003947_23	euxassay_003947_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14170_wholemount_strong.wlz

14170_wholemount_moderate.wlz

14170_wholemount_weak.wlz

14170_wholemount_possible.wlz

14170_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14170_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart atrium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20

heart ventricle

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4941
Entity Detected:	1810031K17Rik, RIKEN cDNA 1810031K17 gene ( MGI:1916421)
Sequence:	sense strand is shown >T4941 CAACTTCGAAAAGTTGTTTCTTCCTTCTTGGAGCGTCGGCCCTCTCTCTTCCCAAAATCTCTCAGCCGCA AATTGTGTGGAAGGAGTGTTTGGAAATAAAACATCCAGGCGCTGTTTGCTTTGGGGAGACCTGTTTAACA TCGTACATTGAGGCTTTATATCTTTTGAGATCATTTAATTGCTGGAGTAACCATACAGAATACAGTATGT ATTCGAGTGTTACAGTTTCAAACTTCTAAGGGGCATAAAGATTCAACAGATTCCCTCCCACCTTGTTCAC AGTTCTCCCACCTACGGGTGGCGGCTGACAGTGCGATGGACCTGACTGGGCTCCTGCTGGACGAAGAAGG CGCCTTCTCCCTCACCGGTTTCCAAGACTTCATGGTCCTTCCAGGACACCAAAAGCTAAGTGCTCGAATC CGAAGGAGACTCTATTATGGCTGGGACTTGGAGACAGACTGCAGCCTGGAGGAGCTCTCCAGCCCTGTGG CAGACATTACTGTGGAACTTCTTCAGAAGGCAGCACCCAGTCCTATTCGCCGACTCCAGAAAAAATATGT AGCCCATGTGTCCCGAGAGGCTTGTATCTCCCCTTGTGCTATGATGCTAGCTCTGGTGTACATTGAGCGG CTCCGGCACCGAAACCCAGATTATCTGCAGCACGTGTCGTCTTCTGATTTGTTTCTGATCTCCATGATGG TGGCCAGTAAATACCTTTATGATGAAGGGGAGGAAGAGGAGGTCTTCAATGACGAATGGGGGGCAGCTGG GGGAGTCGCCGTGGCCACCCTCAATGCCTTGGAAAGGAGCTTCCTGAGTGCCATGGATTGGCGTCTCTAC ACTGATCCTCGGGAGATCTTTGAGGTGCTAAGTTGGCTGGAGAGCTGTGTGGCTGAGCAGCAAGGACGGC GGCGGGGCTGGTACACCTACACAGACCTGTGTGTGCTGCTGGAGCAGCCAACGTGGCAGCTGGCTCTGGG CTCCCTCTGCCAGCGGCTGGTAAAGCTGTCCTGCCTGTTAGCTGTGGCATATGTGAGCAGCGTGGCCCTA GCTGTAGCATCAGTGGCTGTAATACACCAGTCTTTGGGGCTGTCCTCCAGCCCCTCACCAGGTCCTCCTG AACTCACACTGGTCTCCAAGAGCCTCGTGCAGCCCTGCGTACCTGCCCCTGTGTCACAGTGCCTGACTAA TGTCTCCAGCTGCCTGGAAGGCAGTGTAGACTTGCCATCACTCTGGGGCAGTCTTCTGGCCCCATTGACT CCCCCTCTGAGACCTCCCCCAGACCCTCCTGCCCCTCCTACTCCTCTCCACAAATGTCCCCTTTGCCAGA AGTTCCAGAGACTCCCCCCAAACTGCCGTGCCTGCCACACTAACCAAACGGTGTCTATTGGGCCTTCCCG CCCCCTTTACCATGCCCGCGGCCTGGCCCCACCCTGGCTCTGGAGCCCAGTGGCCCCTCCATTTCTCCAG CCCCAGCAGTGTTCGCTTTTTAGTGTCATGGAGCTGGCTCATCTCAAGTCTGTCATTTCCCCAGGCTAGG TAGGGCTGTGAGGAGTACATTGAGAGGGTGTGGGAGTCCTTGAGAACCTGGAAGAGGAAAACTTGATTTA GATTTTTGCTACCTCTCTGGGCCCTTGGCTGGTCCCCTGTCCTCCTGGGTAGGAGCATAAGGGATTATGG GGGCATTACAAGGTCGTTCACTCTCCTAGACCTCAGAGTCTGGCTAGCAGGCTAGGACACCAGTGGTGCC TGGGACAGTGTCAGCTCAGTGACCAGAGGCATGCCACAGATGACAGAGAACCACCTATCTCTCTGCCTCC ACACGCCTAGGGAAGTAGATTTTTTGTTTTGAAGTTTCCAAAGATGGAAAGGTAAAGGTGGGAGTGTGGG AAGCGGAGGAGAGGATTGATGTGGGTCTCTGTGATGTCTCTGAGGCTGAGAACCATGATGAGAGCTGCTG AGGACCAGGAAGCTCCCTGTCACAGCCAAGAATTGCTGGGTCCTCTAACCTTGTCCCTTTGTCTTCCAAC CGTAGCATCCTGCTCCAAGGCTTCCCACTCTGCCTTTTTGGGTGTTCTCTTCACAGGCCTCTCTGGTTAC CACTGCTTTGTATCTGGGTTTTTCACGCTTTTATAGAATTGAGGTTTCAATAAACAGTTTCTGTTGCATG GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5126467 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5126467 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14171 same embryo
	EMAGE:14169 same embryo
	EMAGE:14173 same embryo
	EMAGE:14172 same embryo
	EurExpress:euxassay_003947 same experiment
	MGI:4822639 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source