EMAGE mouse gene expression spatial database

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EMAGE:14171

Hsd17b13 hydroxysteroid (17-beta) dehydrogenase 13 ( MGI:2140804)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003957. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003957_01	euxassay_003957_02	euxassay_003957_03	euxassay_003957_04

euxassay_003957_05	euxassay_003957_06	euxassay_003957_07	euxassay_003957_08	euxassay_003957_09

euxassay_003957_10	euxassay_003957_11	euxassay_003957_12	euxassay_003957_13	euxassay_003957_14

euxassay_003957_15	euxassay_003957_16	euxassay_003957_17	euxassay_003957_18	euxassay_003957_19

euxassay_003957_20	euxassay_003957_21	euxassay_003957_22	euxassay_003957_23	euxassay_003957_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14171_wholemount_strong.wlz

14171_wholemount_moderate.wlz

14171_wholemount_weak.wlz

14171_wholemount_possible.wlz

14171_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14171_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4945
Entity Detected:	Hsd17b13, hydroxysteroid (17-beta) dehydrogenase 13 ( MGI:2140804)
Sequence:	sense strand is shown >T4945 GGCTCTCTGTGGAGCTCTGAGCAGAGGTGTCTGTTGTGAGAACAGAGCCATGAACCTCATCCTGGAATTT CTCCTTCTGGTGGGCGTCATCATCTACTCCTACCTGGAGTCACTGGTAAAGTTCTTCATTCCCCGGAGAA GGAAATCTGTGACCGGGCAGACCGTTCTCATCACGGGGGCCGGACACGGAATAGGCAGGCTGACTGCATA TGAATTTGCAAAGCAGAAAAGCAGACTGGTTCTATGGGATATCAATAAGCGTGGTGTTGAGGAAACCGCG GACAAATGCAGGAAACTGGGGGCCGTCGTGCACGTGTTTGTGGTGGACTGCAGCAACCGGGCCGAGATTT ACAACTCTGTGGATCAGGTAAAGAGAGAAGTAGGTGATGTCGAGATCGTGGTAAACAACGCCGGGGCGAT ATATCCAGCAGACCTTCTTAGTGCCAAGGACGAGGAGATCACCAAGACCTTTGAGGTCAATATCCTCGGA CATTTTTGGATCATAAAAGCACTCCTTCCATCGATGCTGAGAAGAAACTCTGGCCACATTGTCACAGTGG CTTCGGTGTGCGGCCACGGAGTGATTCCTTATCTCATCCCTTATTGCTCCAGCAAGTTTGCTGCTGTGGG CTTCCACCGAGCACTGACCGCAGAACTGGACACCTTGGGGAAAACCGGTATCAAAACCTCGTGTCTCTGC CCTGTGTTCGTGAATACTGGCTTCACCAAAAACCCGAGTACAAGGTTATGGCCTGTATTAGAGCCGGAAG AAGTTGCAAGGAGTCTGATCAATGGAATACTTACCAACAAGAAAATGATTTTCGTTCCATCCTATATCAA TATTTTTCTGATCCTGGAAAAAGGACCTGGATTCAGTTCCAAGCACCCACATGGTGGTTCTCAACAGCCT GTTACTCCAATCCCAGGGGATTTGACACCCTCTTCAGACTTTCTCAAACACTAGGCACTCGTGGGGTACG CATACGTCATTCAGGCAAGCCCTCATACACATAAAATAGAATAAGCACATATTTTAAATGTGCTACTATT ACTGATGTTTTAAGCCAGTTAAATTATGTGATTTTCTTAACAGCAATAAAATATTGTAGATAATGTCATC ATGCTAGCTGCATCACTCTGACTGTATAAACCCAAGAGAATTTCACTCCATCTCCTTCTGCCAAAATATT TACAACACTTAAGAAATAAGGGCTGATTTTTATTTTTTTTAACATAAAATATATGAGACAGTTGTATCCT GTCAGCTAAAAATGTTTTCTCTGGCCTGTGAGTTGGCTCAGTAGATGAAGACACTTGCTGCCAAGCCTAA CAACCTGAATTCAACTCCCAGAACCCATGTAATGGGAAGACAGATGGGATTCAGAGAAATTGTCCTCTGA TCATTGTACCCATGCCTTGGTATATGAGTATCTGTGATGGTTTGTATATGCTCAGTCCAGGGAGTGGCAC TACTAGAAGGTGTGGTCCTGTTGGAGTAGATGTATCACTGTGGGTGTGGGCTTTAATAACCTCATCCTAG CTGCCTGGAAGCCAGTATTCTGCTAGCAGCCTTCATATGAAGATACAGAACTCTCAGCTGTGTTTGCACC ATGCCTGCATGAACGCTGCCATGCTCCCACCTTGATGATAATGGACTGAACCTCTGAACCTGTAAGCCAG CCCCAATTAAATGTTGTCACTTATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5132342 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5132342 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14170 same embryo
	EMAGE:14169 same embryo
	EMAGE:14173 same embryo
	EMAGE:14172 same embryo
	EurExpress:euxassay_003957 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source