EMAGE mouse gene expression spatial database

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EMAGE:14183

Apobr apolipoprotein B receptor ( MGI:2176230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003948. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003948_01	euxassay_003948_02	euxassay_003948_03	euxassay_003948_04

euxassay_003948_05	euxassay_003948_06	euxassay_003948_07	euxassay_003948_08	euxassay_003948_09

euxassay_003948_10	euxassay_003948_11	euxassay_003948_12	euxassay_003948_13	euxassay_003948_14

euxassay_003948_15	euxassay_003948_16	euxassay_003948_17	euxassay_003948_18	euxassay_003948_19

euxassay_003948_20	euxassay_003948_21	euxassay_003948_22	euxassay_003948_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14183_wholemount_strong.wlz

14183_wholemount_moderate.wlz

14183_wholemount_weak.wlz

14183_wholemount_possible.wlz

14183_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14183_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4148
Entity Detected:	Apobr, apolipoprotein B receptor ( MGI:2176230)
Sequence:	sense strand is shown >T4148 GAGACCTGCAGGATGGATTTTCTCCGGCTTCGTCTCCCTGGGCTACATCAGGCTTTGAGGGGAGCACTGG ACTCCTTCAGTGCGTTTGTGTCCTACCTCGTAGGAGACACAGTCCCCACAGTAGAGAGGCAGACACAGGC AGCTGAGGAACTGGGAGAGGTGACTGAGGGGAAGGTTGTAGGAGAGGAAGCCCAGGAGGTACTGGAGGGA CTTAGAAGTGGCCAGAGTGAGGGGGTAGAAGCACCCGAAGAGACTAGAAGATGCCAAGAAGGAAGTTTAG CTGGTGAACAGACCTGGGGGTGGAGAGCAGATAGCTCTGCAAGGCCCCAAGCAGAAAGGCAGGACACTGG GTCCTGGAAGGCAGCTGAGGATGCCAGGGGCCAGGAGCCAAGTGTCCCACTGAAGCCTGAGGCAGAGCCT GGGACTCACAGAGACAGGAGCAGTAATACAGCCCAGGAGATCTGGGAGCACGGTGAAGAGGAAGCAAGCA GTGGGGAGCCTCTGAGAACCTGTGAACAGAAGGAAGAAGAGGAGGAGGTGGTCAGAGCAGCAGAGTCAGG GATGGCCGAGGGGGTGGAGTCACAGCCAACCTGGCACAGCGAGCCTGGGGGGAATGCTGGCACTGAGGGG CAGCATGTGACAGAGGACAGCAAAGAGATAGACTGGGTGGCCAAAGACATGGTTGCAGAAATCGAATGGT TTGGTGCCAAAGGGATTGACAAGGAAGAGGAGAGGATGGTCCCAATGAGGGATGGTGAAAGGGCAAGGGC ACAGGGGACACAATGCCCCGGGGCCGAGTCTGAGGACCAGGCCATGTTGAGTAGAGAGGCCTGGACAGTC TCAGACAGGGAGGGGGCTGATAGCCTAGGAGTTCAGGGAACAGAATACGGGTCAGACCCAGGAGACAACT TCCCAGGAACTACTGGGAGAGTCTGGGTCCTAGAAGAGGCTGACAAGGGAGACCAGCAGGATGAGGTCGA CGAGAAGAGAGAGGCTGAAGTTAGGTTTCCGATCCAGACCCTGGAGGCTGAGAGAACTGGAGAGATGACT GAAGGCCACATAGCTGAAGAGGAGGCTATGGGAGAACAGGAAACAGAGGGCAGCTTTGAGGATGAGGAGA GGCAAGACTTAGCCATCAGGGATAATGGAGTGAGTCTAGAAGAAGAGGTACGGGCAGAAGAATCCTCCAG GGAGAAAAGGAACTCCTGGGCCACAGAGCCTACGCTAGTCCTAGACACGGAGGCTAAAGATGAACCTGAC TGGGAAGACTCTCCAGAAGTCAGCACTGAGGAGTTGTTTGTGGGAGAGAGGAGTGAAGCCGCCCAGATGA CACCAGAGGTATTGAGAGTAAAGGTCACTGAAGGACAGGACCCTGAGCTGGTGAGACATTCCCAGGCTTT AACTAAACAACTTGAGGAAGGACAGAAGGGTCAGGAAGAGACTAGTGGAGCTCCAGACCTGAGCCCAGAG CGGGTGCTAAGCTTGAAGGAATATCCTGGGCCTGTGGGGTTTGCAGGCCCTGAGCTAGAAGCCTGGGGAA ACTGGAGTAGGGGTGTGGACCGCAGAAATAGCCAGGAGGTAAAAGCAGATGCTGAAGCAGGCAAGGAACA GACTGCAACCGAGCAGGCAGTGGAGATCCGGGCTGAAGGAGGCCAGGAGGCCCAACAGCCAGAGGTCTTC GGGTCAGGAGGAGAGGAGGCACTGACCTCCGTAGCACTTAACCCGGAGCTGGAGGGAAGCCAAGGAGCAG AGGCGGGGACAGAAGAGTCAGTGGAGGAATCCAAGCCCACAGAAAACGAGGCTGCTGAGGAGGAGGCTGT AGTGCCTTGGGAGGCAGATGGAACTTGCAGGAAGAGGAGGCTGGAGGAGGTGACTCTGAGCCTGCAAGAC AGTGAGGACACAGAGACCAGTTATTTGGCTGAGGAGATTATTGTGGGTATCAGGGCCGTGGATACCGAGG AAGGGCCAAAATGGGAAGCTGGGTTGGCTCCAGAAACAGAGCTTGGGAAAGCCTGGTGTTCCGAGGGCAG AGGGGAGGCTGGGAGAGGCACGGAGCTGGAGGAGACTACAGAGAAGCAAAGTGGACAGGAAGTTGGCTTG GTGGGCTCAGCAGAGAAGGTGTCTGGCTATGATATCCAAGAAATTGATGGGACTGAAGAGGGGGAGCAGG CAGAGATGGAGACATCTGTAATGGCAGAAGACATAAGGGGGACGGATGGTGTGACTTTAGGCTCCCAGGC AGAGAGAGCAGAGGGGTCCATAACCCCCATGGAGACTGAGGGGCTCCTAAGAGATCAGATGCTGTTGGAA GAAGAGGCTGGGGGAGGGCAGTCGCGAGAGCAGAAGGTCCACAACTCAGAGGGCGAGATCCAAACGCTGG ACGACTCTTCAGACCAGGAGGGGCAGCAGACACACCAAATCCCCACAGTAGCTGTGCCTGGGCCCTTGGA ATCGGCTGAGGCCACGGCAGGTGCCCCAGGGGATGTTCACAGCAACTGGAATGAGGCCCTGCTCCCTGGG TCCCGCCTGGATGTCTCTGTCCCTCGGAGTCGTGTGCTCCTCTCTCGAAGCTCCTCACGGCGGCGCTCGA GGCCCTCTTTCCATCGAATCTCTGTTCCTGAGCCACAGTGTGACCCTCCCAGCCCCCAACCCCAGGCGGA GCGGCCGGTCCCTGAGCAATCCTCTCTTCAGCTAGAGGAAACTCCAGAGCTAAGTGCCACAAAGCCCGAG GGGACTCCAGTGCCAGCCAGAAGAAAAATGCTGGGGCGTGGGTTTGGCTTTGCTCATCCTGGCATGATGC AGGAGCTGCAAGCCCGACTGAGCCAGCCAAAGCCACAGTGACAGGGCCTAGAGCCCTAGAGAGAGAGACC TCCCAAGAGAGTCCGAAGGCTCTTCTGGACCCTGGTTACTCTTGAGTCCCTCGGCCACTGGAATATATCT TTTCACCCTCCCGGCCTCAGCTAAACTGGATATTTATGAAGACCTTGAATCTAGGAGTCTGATTAAGCCA GTGAGGAACCTGCTCCACTGGCATCGATCATGGATTCCTTTCCCGGTGCCCTTTCACATCTGTCTCAGCT ACTCGGAAGGCTACCCGGAAGACTGGGCTGGGGAAACTATTTTCCCCTAAATTTCCCCAGTATGCTCACC TGCCACCCAATGTGAGATAAGACCAGCAAGTGTAAGTGTGCTTGCGTGTAAGATAGCACACTTCTGGGGT GGAAACCAGCCCAAAGGAAAGAGGTTCAAGGGGCTGAGCAAGGGACACCACTTGGAGTCCACGGCCACCG GCAACAGATCAGTTGCTTTGAGTTTCCAAAATGACGGCTCGGTATCACAGCTCTGCAGGTTACTATGCTA ACCTCCATGCCAGAGTTGGGGAACGTTGCCCAGGAACTCCAAGCACATAGGAGTAGAGAGGGGTTCTGTG TGGCTGGAAGCTTCCCCCTCCCCCCAACTCCATCCCAAACCGGCCTGTTCCTTGGGTGTCAGACCTTCCT GTGAAAAGATGTGGAAACACAAACCGGGCTGGATGGCTTTATTAGGCACCCAGCATGGTGGCCTAACTCA AGAGTGAAACAATAAATAAACCATTTCTTAAAAATAAATAAATATCCAAGAAATAAATATCTGGTAAGGA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4482457 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4482457 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14182 same embryo
	EMAGE:14180 same embryo
	EMAGE:14179 same embryo
	EMAGE:14184 same embryo
	EMAGE:14181 same embryo
	EurExpress:euxassay_003948 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source