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EMAGE:14243

Cst10 cystatin 10 (chondrocytes) ( MGI:1930004)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14243
euxassay_003951_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14243Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14243_wholemount_strong.wlz
14243_wholemount_moderate.wlz
14243_wholemount_weak.wlz
14243_wholemount_possible.wlz
14243_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14243_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4398
Entity Detected:Cst10, cystatin 10 (chondrocytes) ( MGI:1930004)
Sequence:sense strand is shown

>T4398
TTTCTTCTCTGCTCCTCTAAAGCTTCATCAACCATGGCAAGTTTGCTGAGCCCCTCGATGCCTGTGTTGG
CTGCTGTGGCCTTGACACTGACCCTGGCTGTGATCCCTGAAGCCAGTACTAATGCTGAAGCCAAGCAGGT
GGTATTAGGTGGAGTGGAGCCAGCTGACCCTAAGGATAAGGAGGTGCAGAAAGTGGTAAAGTTTGCTGTT
AGAACCTACAATGATATGGACAATGACCTGTATCTTAGCAAGCCAATACGACTGATGAGCGCCAGCCAGC
AGGTTGTGGCTGGGAAGAACTACTACTTGAAAATTGAGCTAGGCCGAACCACATGTACCAAAACTGAGTC
TAATTTGGTTGATTGTCCCTTCAATGAACAGCCAGATCAGCAGAAGAGGGTCATCTGTAATTTCCAGATC
AACGTTGCACCTTGGCTGAACAAGATGTCCATGACAAACTTCAACTGTTACAATTTCTGAGGATATATGT
CAGGCTACACTTCTGCTGATTGTCTCTTACTTTTACTCAATCTTATCCTTAAGTTCTCCTTTCCTCAGAC
AGAGATCTTAGTCCCAAAGGTTGTCTCTTGAGAATGATAGCACCAAGAGAAAAGTAGAGATAGGCCCTGC
ACACCTAAGTGACAGTTCCCTTGTTCCTTTCATCTACTTGTTTTTCAAAGGCCCACTGTTCATTATTTGC
TCAATAAAAAAATAGCCATAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4920219 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4920219 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14245 same embryo
 EMAGE:14244 same embryo
 EMAGE:14242 same embryo
 EMAGE:14247 same embryo
 EMAGE:14246 same embryo
 EurExpress:euxassay_003951 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS