EMAGE mouse gene expression spatial database

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EMAGE:14245

Hepacam2 HEPACAM family member 2 ( MGI:2141520)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001780. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001780_01	euxassay_001780_02	euxassay_001780_03	euxassay_001780_04

euxassay_001780_05	euxassay_001780_06	euxassay_001780_07	euxassay_001780_08	euxassay_001780_09

euxassay_001780_10	euxassay_001780_11	euxassay_001780_12	euxassay_001780_13	euxassay_001780_14

euxassay_001780_15	euxassay_001780_16	euxassay_001780_17	euxassay_001780_18	euxassay_001780_19

euxassay_001780_20	euxassay_001780_21	euxassay_001780_22	euxassay_001780_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14245_wholemount_strong.wlz

14245_wholemount_moderate.wlz

14245_wholemount_weak.wlz

14245_wholemount_possible.wlz

14245_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14245_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

weak

regional

weak expression: see section 08 09 10 11

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 10 weak expression: see section 08 09

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 13 14 weak expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4162
Entity Detected:	Hepacam2, HEPACAM family member 2 ( MGI:2141520)
Sequence:	sense strand is shown >T4162 CGCATGCATGGGACAGGATGCTTTTATGGAGCTTCTCAGAAGCATGGTTGGGCTCTCTCTTTGCAAAATA CACCTTCTTCTTATAGCAGGTTCTTGTCTGGGACTGAAGGTGACCGTGCCGTCATACACTGTCCACGGCA TCAGGGGACAGGCCCTCTACCTTCCAGTGCACTATGGCTTCCACACTCCGGCATCTGACATCCAGATCAT ATGGCTGTTTGAAAGATCCCACACAATGCCCAAATACTTGCTAGGCTCCGTTAATAAGTCTGTGGTTCCT GACTTGGAATACCAGCACAAGTTCACCATGATGCCACCCAATGCATCTCTGCTCATCAACCCGCTGCAGT TCACCGATGAGGGCAATTACATCGTGAAGGTCAACATCCAGGGGAATGGAACTCTGTCTGCAAGTCAGAA GATACAAGTCACTGTCGATGATCCTGTCATGAAGCCAATGGTTCAGTTCCATCCTGCTTCTGGGGCTGTT GAGTATGTGGGCAACATTACCTTGACCTGCCAGGTAGAAGGAGGTACCAGGCTGGTTTACCAGTGGCGAA AAAGTGGGAAACCCATTTCCATCAACTCCAGCCACTCTTTTTCTCCCCAAAACAACACCCTTTGGATTGT TCCAGTGACAAAAGAAGACATTGGAAACTACACCTGCCTCGTGTCAAACCCCGTCAGTGAGATGGAAAGT GATATCATTATGCCCACCATATATTATGGACCTTATGGACTTCAGGTTAATTCTGATAAAGGGCTAAAAG TGGGAGAAGTATTCACTGTTGACCTGGGAGAAGCTGTCCTCTTTGACTGTTCAGCTGATTCTTATCCCCC TAACACCTACTCCTGGATCCAAAGATCTGACAATACTACACATGTCATTAAGCATGGGCCTCATTTAGAA GTTGCATCTGAGAAAGTGGCCCAGAAGACAGCTGACTATGTGTGCTGTGCTTACAACAACATCACCGGAA GACGCGATGAAACTCGATTCACAGTCATCATAACTTCCGTAGGACTGGAGAAGCTTGCACAAAGGGGAAA ATCCCTGTCCCCACTAGCAAGTATAACTGGAATATCACTGTTTTTGATTATATCCATGTGCCTTCTTTTT CTGTGGAAAAAATATCAACCTTACAAAGCTATAAGACAGAAGCTAGAAGGCAGACCAGAGTCAGAATACA GAAAAGCTCAAACATTTTCAGGCCATGAAGATGCCCTGAGTGACTTCGGGATCTACGAGTTTGTGACTTT TCCGGATGCATCTGGTGTTTCCAGGATGTCAAGTAGGTCTTCCCCTGCCTCTGATGGTGTAACAGGGCAA GATATTCACGGAACAATTTATGAAGTCATTCAGCATATCCCAGAACAACAGCAAGAAAATACAGAGTGAG ATTCAATGGGCAAAATAGTATATTGGAGTAAAATCACAAAGAAACATTTTGAAAGGAAACATCTGGAATC TGTGAAAAATACAATCCTAGCAACCTTCAGTTACATCTTGATGCAGAGTAGAGGCGCCTGTGCAGACTGA AGGCTGGTTTTCTGACGTGCGCAATGGGCTGTGTGACAGAGGAACACCGTAGGAATATTCTTCCGTGGAC AGTTTATCACCCAGACTGGGAGGAAGAAGAGAACGAAGCTCCCTAGTTACTTGCTACTGAAACTTCCAAG CGTTCCAACTGGGGCTCGCCCATGCTCCTCCACTCGGATCAGTGAAGCACTACTCTGTGTCACCTGCAAA CTCCGGGGTTTCACCTTGGTAGATGTTCGCAATAAGCTTTGTTTTATTAAGTGCTCATTACTGTTATGAA AGTAAAACCACCTTTTTTTTCCCTTTGTCCCAAATTGATTTCTTGTTGTTTGTGTCACAAAAAATTAAGG ATGCTTAACACAGAGGTAAGACTGTGCCTTTTCCCTGGCTCTCAGTGAACTTTGGTATCAACAGTGTATT TGGAAAGACAGGCAAAATGGTTTTTTGTTTGTTTGGTTGGTTTGTTTTGGTTGGTTTGTTTGGTTGTTTT GTTTGGTTTGTTAAAATCTCATTTTGATTTTTGTTCAAAGAAAGAAAAAATTGAAATAAAGTATTCTGTT TGCCTTTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4483627 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4483627 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14244 same embryo
	EMAGE:14242 same embryo
	EMAGE:14247 same embryo
	EMAGE:14246 same embryo
	EMAGE:14243 same embryo
	EurExpress:euxassay_001780 same experiment
	MGI:4825354 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source