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EMAGE:14257

Nipsnap3b nipsnap homolog 3B (C. elegans) ( MGI:1913786)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14257 EMAGE:14257 EMAGE:14257 EMAGE:14257 EMAGE:14257
"Pseudo-wholemount" of euxassay_005569. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005569_01 euxassay_005569_02 euxassay_005569_03 euxassay_005569_04
EMAGE:14257 EMAGE:14257 EMAGE:14257 EMAGE:14257 EMAGE:14257
euxassay_005569_05 euxassay_005569_06 euxassay_005569_07 euxassay_005569_08 euxassay_005569_09
EMAGE:14257 EMAGE:14257 EMAGE:14257 EMAGE:14257 EMAGE:14257
euxassay_005569_10 euxassay_005569_11 euxassay_005569_12 euxassay_005569_13 euxassay_005569_14
EMAGE:14257 EMAGE:14257 EMAGE:14257 EMAGE:14257 EMAGE:14257
euxassay_005569_15 euxassay_005569_16 euxassay_005569_17 euxassay_005569_18 euxassay_005569_19
EMAGE:14257 EMAGE:14257 EMAGE:14257
euxassay_005569_20 euxassay_005569_21 euxassay_005569_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14257Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14257_wholemount_strong.wlz
14257_wholemount_moderate.wlz
14257_wholemount_weak.wlz
14257_wholemount_possible.wlz
14257_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14257_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2693
Entity Detected:Nipsnap3b, nipsnap homolog 3B (C. elegans) ( MGI:1913786)
Sequence:sense strand is shown

>T2693
ATTTTGCTCATCGAACTGCAGTCCGCAAAGCCTTGGCCAAGGATAAGGAATGGCAGGAACGATTCCTCAT
TCCAAATTTGGCTTTCATTGATAAACAAGAGGTTGAGATTACCTACTTGGTACCATGGTGCAAAATAGGA
ACGCCTCCCAAGGAAGGAGTCTATGAACTGGCTACCTTTCAGATGAAGCCTGGCGGCCCAGCTCTGTGGG
GTAATGCATTTAAAAGGGCAGTAAATGCCCATGTGGAGCTTGGCTACTCTACACTAGTTGGTGTTTTCCA
CACCGAATATGGAGCCCTCAACAGAGTTCATGTTCTGTGGTGGAACGAGAGTGCAGACAGCCGAGCAGCT
GGGAGACACTGGTCTCACGAGGACCCTAGAGTGGTGGCTGCTGTTCGGGAGAGTGTCAGTTACCTAGAGT
CCCAGCAGAATACGTTCCTGATTCCAACATCATTTTCACCACTGAAGTAGTTTCCTACCCAGCCCAGAGC
ATCCCATCACAGAGGTGAGCTGTATCCACAGCGGGCACTTCCTG
Notes:The probe template was PCR amplified from IMAGE:1512092 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1512092 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14259 same embryo
 EMAGE:14260 same embryo
 EMAGE:14258 same embryo
 EMAGE:14256 same embryo
 EMAGE:14261 same embryo
 EMAGE:14262 same embryo
 EurExpress:euxassay_005569 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS