EMAGE mouse gene expression spatial database

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EMAGE:14300

Chd4 chromodomain helicase DNA binding protein 4 ( MGI:1344380)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004098. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004098_01	euxassay_004098_02	euxassay_004098_03	euxassay_004098_04

euxassay_004098_05	euxassay_004098_06	euxassay_004098_07	euxassay_004098_08	euxassay_004098_09

euxassay_004098_10	euxassay_004098_11	euxassay_004098_12	euxassay_004098_13	euxassay_004098_14

euxassay_004098_15	euxassay_004098_16	euxassay_004098_17	euxassay_004098_18	euxassay_004098_19

euxassay_004098_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14300_wholemount_strong.wlz

14300_wholemount_moderate.wlz

14300_wholemount_weak.wlz

14300_wholemount_possible.wlz

14300_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14300_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5601
Entity Detected:	Chd4, chromodomain helicase DNA binding protein 4 ( MGI:1344380)
Sequence:	sense strand is shown >T5601 CGGAGCCGGAGCCATTTTCCCCCCTTCGGCCGCGGCGAGGAGGAGCTGGAGCGGGAGTGACACCGGCGAG ACCCAGCGCGACCTGAGGCGGCTCTAGGTGACTCGGGCCAGTGCAGAGGGCCCCAGGCCGCAGGCAGGAG CAGCTGGGCCAATTCCCTGGCCGGGGAACGGAAGGGGATGGCGTCGGGCCTGGGCTCCCCGTCCCCCTGC TCAGCGGGCAGTGAGGAGGAGGATATGGACGCACTTCTGAACAACAGCCTGCCCCCACCCCATCCAGAAA ATGAGGAAGACCCAGACGAGGATTTATCCGAAGCAGAGACTCCAAAGCTCAAGAAGAAGAAAAAGCCTAA GAAACCTCGAGACCCTAAAATCCCTAAGAGCAAGCGCCAAAAAAAGGAGCTGGGGGACAGCTCTGGGGAG GGGCCAGAGTTTGTGGAGGAGGAGGAAGAGGTGGCTCTGCGCTCAGACAGTGAGGGCAGCGACTATACTC CTGGCAAGAAGAAGAAAAAGAAGCTCGGACCTAAGAAAGAGAAGAAGAGCAAATCCAAGCGGAAGGAGGA GGAGGAAGAGGAGGACGAGGATGATGACTCGAAGGAACCTAAGTCATCAGCCCAGCTTTTAGAAGACTGG GGTATGGAAGACATCGACCATGTGTTCTCAGAGGAGGATTATCGGACCCTCACCAACTACAAGGCCTTCA GCCAATTTGTTAGACCCCTGATTGCTGCTAAAAACCCCAAGATTGCTGTCTCCAAGATGATGATGGTCTT GGGTGCAAAGTGGAGGGAATTCAGTACCAACAACCCCTTCAAGGGCAGCTCCGGAGCATCAGTGGCAGCT GCGGCGGCGGCGGCTGTGGCTGTGGTTGAGAGCATGGTGACAGCTACTGAAGTCGCCCCTCCCCCTCCCC CTGTGGAGGTGCCGATCCGCAAGGCCAAGACCAAGGAGGGCAAAGGTCCCAATGCTCGGAGGAAGCCGAA AGGCAGCCCGCGAGTGCCTGACGCCAAGAAGCCCAAACCCAAGAAAGTAGCTCCTCTGAAGATCAAGCTG GGAGGGTTCGGCTCCAAGCGGAAGAGATCCTCGAGTGAGGACGACGATTTAGATGTAGAGTCTGACTTCG ACGATGCCAGTATCAACAGCTATTCTGTTTCGGATGGTTCCACCAGCCGCAGTAGTCGGAGCCGTAAGAA ACTCCGGACCGCTAAAAAGAAAAAGAAAGGCGAGGAGGAGGTGACTGCTGTGGATGGTTATGAGACAGAC CACCAGGACTATTGCGAGGTGTGCCAGCAAGGCGGCGAGATCATCCTGTGTGATACCTGTCCCCGAGCCT ACCATATGGTGTGCCTGGACCCAGACATGGAGAAGGCCCCGGAGGGCAAGTGGAGCTGTCCGCACTGTGA GAAGGAGGGGATCCAGTGGGAAGCTAAGGAGGACAATTCTGAGGGCGAGGAGATTCTGGAAGAAGTCGGG GGGGACCCAGAAGAGGAGGATGACCATCACATGGAATTCTGTCGCGTCTGCAAGGACGGCGGGGAGCTCC TGTGCTGTGACACATGCCCTTCTTCCTACCACATCCACTGCCTGAACCCCCCGCTGCCAGAGATCCCAAA CGGCGAATGGCTCTGTCCCCGCTGTACTTGTCCAGCTCTTAAGGGCAAAGTGCAGAAGATCCTAATTTGG AAGTGGGGCCAGCCACCGTCTCCCACACCAGTGCCTCGGCCTCCAGATGCTGACCCTAATACCCCGTCGC CCAAGCCCTTGGAGGGGCGGCCAGAGAGGCAGTTCTTTGTGAAATGGCAAGGCATGTCTTACTGGCACTG CTCCTGGGTGTCAGAACTGCAGTTGGAGCTGCACTGTCAGGTGATGTTCAGAAACTACCAGCGGAAGAAT GATATGGACGAGCCGCCTTCTGGGGACTTTGGTGGAGATGAAGAGAAGAGCCGGAAGCGAAAGAACAAGG ACCCTAAGTTTGCAGAGATGGAAGAGCGCTTCTACCGCTATGGAATAAAACCGGAGTGGATGATGATCCA CCGCATCCTCAACCACAGTGTGGACAAGAAGGGCCACGTTCACTATTTAATCAAGTGGCGAGACCTGCCC TATGACCAGGCGTCCTGGGAGAGCGAGGACGTGGAGATTCAGGACTACGACCTGTTCAAGCAGAGCTACT GGAACCACAGGGAGTTAATGAGGGGTGAAGAAGGACGACCCGGCAAGAAGCTTAAGAAGGTGAAGCTACG GAAACTGGAAAGGCCTCCGGAGACCCCAACTGTTGATCCCACCGTGAAGTATGAGCGACAGCCGGAGTAT CTGGATGCGACGGGTGGAACCCTGCATCCCTACCAAATGGAAGGCTTAAACTGGCTCCGCTTCTCCTGGG CTCAGGGAACCGATACTATCTTGGCTGATGAGATGGGCCTTGGGAAAACTGTGCAGACAGCAGTCTTCCT CTACTCCCTCTATAAGGAGGGTCACTCCAAAGGCCCCTTCTTAGTGAGCGCTCCTCTGTCCACCATCATC AACTGGGAGAGAGAGTTTGAGATGTGGGCTCCTGATATGTATGTGGTAACCTATGTGGGTGACAAGGACA GCCGTGCCATCATCCGGGAAAATGAGTTCTCCTTTGAAGACAATGCCATTCGTGGTGGCAAGAAGGCCTC GAGGATGAAGAAAGAAGCATCGGTGAAATTCCATGTTCTGCTGACATCCTATGAGTTGATCACCATTGAT ATGGCCATCTTGGGTTCTATTGACTGGGCCTGCCTCATTGTGGATGAAGCCCATCGGCTGAAGAACAACC AGTCTAAGTTCTTTCGAGTTTTGAATGGTTACTCACTCCAGCACAAGCTGTTGCTAACTGGAACTCCGTT ACAGAACAATCTAGAGGAACTGTTTCATCTGCTCAACTTTCTCACCCCTGAGAGGTTCCACAACTTAGAA GGCTTCTTGGAGGAGTTTGCAGACATTGCCAAAGAGGACCAGATTAAAAAACTCCACGACATGCTGGGGC CTCATATGTTGCGGCGGCTCAAAGCTGACGTCTTCAAGAATATGCCATCCAAGACAGAGCTGATTGTGCG CGTGGAGTTGAGTCCTATGCAGAAGAAGTACTACAAGTATATTCTCACGCGGAATTTCGAAGCACTTAAT GCTCGAGGTGGTGGCAACCAGGTTTCTCTGCTGAACGTGGTGATGGATCTTAAGAAATGCTGCAACCACC CTTATCTCTTCCCTGTGGCAGCAATGGAAGCCCCTAAGATGCCTAATGGTATGTATGATGGCAGTGCCCT AATCAGAGCATCTGGGAAGTTGTTGCTGCTGCAGAAGATGCTTAAGAACCTGAAAGAGGGAGGGCATCGT GTGCTCATCTTCTCCCAGATGACCAAGATGTTGGACTTGCTAGAGGATTTTTTAGAACATGAAGGTTATA AATATGAACGTATTGATGGTGGAATCACTGGGAACATGCGTCAGGAGGCCATTGATCGCTTCAATGCACC GGGTGCTCAACAGTTCTGCTTCTTGCTTTCCACTCGAGCTGGGGGCCTTGGGATCAATCTGGCCACTGCG GACACAGTTATTATATATGACTCTGACTGGAACCCCCATAATGACATCCAGGCCTTTAGCAGAGCCCACC GTATTGGGCAAAATAAGAAGGTGATGATCTACCGGTTTGTGACCCGTGCATCAGTGGAGGAGCGCATCAC GCAGGTGGCAAAGAAGAAGATGATGCTGACACATTTAGTGGTACGGCCTGGCCTGGGCTCCAAGACAGGC TCAATGTCTAAACAGGAGCTTGATGATATCCTCAAATTTGGCACTGAGGAGCTATTCAAGGATGAAGCCA CGGATGGAGGAGGAGACAACAAGGAGGGAGAAGACAGCAGTGTTATCCACTATGACGATAAGGCCATTGA ACGACTGCTGGATCGAAACCAGGATGAGACTGAAGACACAGAATTGCAGGGCATGAATGAATATTTGAGC TCATTCAAAGTGGCTCAGTATGTGGTACGGGAAGAAGAGATGGGGGAGGAAGAGGAGGTAGAACGGGAAA TCATAAAACAGGAAGAAAGTGTGGATCCTGACTACTGGGAGAAATTGCTGCGGCACCATTATGAGCAGCA GCAAGAAGATCTAGCCCGAAATCTGGGCAAAGGAAAAAGAATCCGTAAACAGGTCAACTACAATGATGGC TCACAGGAGGACCGAGATTGGCAGGACGACCAGTCCGACAACCAGTCCGATTACTCAGTGGCCTCAGAGG AAGGTGATGAAGACTTTGACGAACGGTCAGAAGCTCCCCGCAGGCCCAGTCGCAAGGGCCTGCGGAATGA TAAAGATAAGCCATTACCTCCTCTGTTGGCCCGTGTTGGTGGGAATATTGAAGTACTTGGTTTTAATGCT CGTCAGCGAAAAGCATTTCTTAATGCAATTATGCGATATGGCATGCCACCTCAGGATGCTTTTACCACTC AGTGGCTTGTGAGAGATCTTCGAGGCAAGTCAGAGAAAGAATTTAAGGCTTATGTGTCACTCTTCATGCG ACATTTGTGTGAGCCTGGGGCAGATGGGGCTGAGACCTTTGCTGATGGCGTCCCACGAGAAGGCCTGTCT CGTCAACATGTTCTCACTAGGATCGGTGTCATGTCTCTGATTCGAAAGAAGGTTCAGGAGTTTGAACATG TTAATGGACGCTGGAGCATGCCCGAACTGGCTGAAGTAGAGGAAAACAAAAAAATGTCTCAGCCAGGTTC ACCTTCTCCAAAGACTCCTACACCTTCCACACCAGGGGACACACAACCCAATACTCCTGCGCCTGTGCCA CCTGCTGAGGATGGGATAAAAATAGAGGAAAATAGCCTTAAAGAAGAAGAGAGCACAGAAGGAGAGAAGG AAGTTAAATCTACAGCCCCTGAGGCGACTGTGGAGTGTGCACAGCCCCCTGCCCCTGCCCCTGCCACTGC CCCTGCCACTGCCACTGCCCCTGAGGATGACAAGGCTCCTGCTGAGCCTCCTGAGGGGGAGGAAAAAGTG GAAAAGGCAGAGGTGAAGGAGAGAACAGAGGAGCCTATGGAGACAGAGGCCAAAGGTACTACTGAGGTGG AGAAAGTGGAGGAGAAGTCTGCGGTAGACCTGACCCCGATCGTGGTGGAAGACAAAGAAGAGAAGAAGGA GGAGGAGGAGAAAAAGGACGTGATGCTTCAGAATGGAGAAACTCCCAAGGATCTTAGTGATGAGAAGCAG AAGAAAAACAGCAAACAGCGTTTCATGTTCAACATTGCAGATGGTGGTTTTACTGAGTTGCACTCACTGT GGCAGAATGAAGAGCGGGCAGCCACAGTCACTAAGAAGACTTACGAGATCTGGCACCGTCGCCATGATTA CTGGCTGCTGGCTGGCATTATAAACCACGGCTATGCCCGGTGGCAGGACATCCAGAATGACCCACGGTAT GCCATCCTCAATGAGCCTTTCAAGGGTGAAATGAATCGTGGCAATTTTCTAGAGATCAAGAATAAATTTT TAGCTCGAAGATTCAAGCTCTTAGAACAAGCCCTGGTGATCGAGGAGCAGCTTCGCAGGGCAGCTTACCT GAACATGTCCGAGGACCCCTCTCACCCGTCTATGGCCCTTAACACCCGCTTCGCTGAGGTGGAGTGTTTG GCAGAAAGTCACCAGCACCTGTCCAAGGAGTCGATGGCAGGGAATAAGCCGGCCAATGCAGTCCTACACA AAGTCCTGAAACAGCTGGAGGAACTGTTGAGTGACATGAAAGCTGATGTGACGCGGCTCCCAGCCACCAT TGCCCGCATCCCCCCAGTCGCTGTGAGGTTACAGATGTCAGAGCGGAACATTCTCAGCCGCCTGGCAAAC AGGGCGCCAGAACCTCCCCCACAGCAGGTAGCCCAGCAGCAGTGAAAGACCCAGAGTGATATCCACCTCC ACCACTCAGCAGTGACCTTCCTCACTTTCTCTTGTCCCGGCTGCCCCTGGGGGCCAGAGAGACCCTTGCC TTCCTTCTGCCCATCTTCTGAGTTGTAAAGGAACAGCCCTGCGCAGTGGGGCAGGGAGGGAGGGAGAAGC AATGGTGCCCTTCCTGCTGGAGAGACAAGCAGCAGTAGCGCAGCGCCCGCCCGCCATGTGCAGGAGCTGG CGGGCTGGCTGCCTTCTGGACCCTGGCTTCTCCCCACCGTGCGCCTGTTACAAACTGTTGTGGATTCTGC CAGGCTCGAAGAAAATGATCTGAATTTCTTCCTCTTTTTGGTTTTATTTTGTTGGTTTACTTTGTGTTTT CTCCTTTTTGGGGGGTATTCAGAGTGGGCCGGGCCCCTGGGCGAGACACAGCTACCTCTGTTGGCATCTT TTTAATACCAGGAACCCAGCGGCTCCAGCCACTGAGCAGCTAAATAAAGTTGGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6489649 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6489649 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14297 same embryo
	EMAGE:14296 same embryo
	EMAGE:14299 same embryo
	EMAGE:14301 same embryo
	EMAGE:14298 same embryo
	EurExpress:euxassay_004098 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source