EMAGE mouse gene expression spatial database

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EMAGE:14301

BC046331 cDNA sequence BC046331 ( MGI:2687282)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004149. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004149_01	euxassay_004149_02	euxassay_004149_03	euxassay_004149_04

euxassay_004149_05	euxassay_004149_06	euxassay_004149_07	euxassay_004149_08	euxassay_004149_09

euxassay_004149_10	euxassay_004149_11	euxassay_004149_12	euxassay_004149_13	euxassay_004149_14

euxassay_004149_15	euxassay_004149_16	euxassay_004149_17	euxassay_004149_18	euxassay_004149_19

euxassay_004149_20	euxassay_004149_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14301_wholemount_strong.wlz

14301_wholemount_moderate.wlz

14301_wholemount_weak.wlz

14301_wholemount_possible.wlz

14301_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14301_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5585
Entity Detected:	BC046331, cDNA sequence BC046331 ( MGI:2687282)
Sequence:	sense strand is shown >T5585 GTTGCCACGAGACTAAAGACTGTAGCTCTGGCTCCGGAGCTCTTCCCTGCTCTTCTGGACAGCCAGTGTG TGCAGAATGCCCCACAAGATTGGATTTGTAGTGGTCAGCTCCTCTGGACATGAAGATGGCTTCAGTGCCC GAGAACTAATGATCCACGCACCAACCGTCAGTGGGTGGAGATCACCAAAATTCTGCCAGTTTCCACAAGA AATCGTTCTCCAGATGGTGGAGAGGTGTCGAGTAAGAAAGCTGCAGTTACTTGCTCACCAGTACATGATC TCTAGCAAAGTCGAGTTCTACATCAGTGAAAGCCTGCCTGAGTACTTGGTGCCATACCAAGCAGAGAGGT TCCGCAGACTGGGCTATGTCTCCCTCTGTGATAATGAGAAGACAGGCTGCAAAGCCCGGGAGCTCAAGTC TGTTTATGTGGATGCTGTGGGGCAGTTTCTTAAACTGATTTTTCACCAAAACCATGCCAACAAATACAAC ATATATAATCAGGTGGCTTTGGTTGCAATAAATATCATTGGAGACCCTGCAGATCTGGGTGATGAAAGCA ATATTACCTCTAGGGAGAAGCTGATCGACCACTACCTGGGCCACAGTCCCCACAATCCTGAGGACCCAGC CCTAGATGGAACTTTCTCTGGGCGATCTGACTACATCTCTCCACTTGATGACTTGGCTTTTGACATGTAC CAAGACCCTGAAGTTGCCCAGATCATCCGCAGGCTGGATGAAAGGAAACGGGAAGCTGTCAAGAAGGAGC GCTATGACCATGCAAAGAAACTGAAGCAAGCGATCGCTGACTTGCAGAAGGTCGGTGAGCGCCTAGGGCG CTATGAAGTGGAGAAGCGCTGTGCGGTGGAGAAGGAAGACTACGATCTGGCCAAGGAGAAGAAACAGCAG ATGGCTCGCTACCGTGCCCAGGTGTACGAACAGCTGGAACTACATGGCCTTCTGCAGGGCGAGCCAGAGA TGCAGAGGCCATTCGCCTTGCCCCTCCAGCCCCTTGCATCTCCCAGCAGTCCCCAGCACTGGAAGGCCGT GTCCTCACTCCCTCGCACAGAAAAGCTGGTAGCTGAAGACTCGTTTGCTGGCCCTGTCCTCCAGGAGAAG CCCTCAGCATCTTCTCCTCAGCATTCAGCAGTGGACCCGTCCCCACCTGCTGCAGGCCACGCCCCAAGGA GCCATACGGAAGTCCTGCCCTATGATGAACGGCCTCTTCCTGTTACTCGGAAGCAGTTGGGGGAAGCATC TGCAGAGGCAGAAGTGAAAGAGGCAGACAGTGATGTCCGGAGGCGGGGTGTCGCAGGGGAGCCAGAGCCC TTGACAGAGAAGGCCTTGAGAGAAGCCAGTGCTGCCATCGACACCTTAGGCGAAGCCTTGGTGGCTGGGG CCTATTCTAAGATGTGGTCCTGCCGAGAAGATGCACTACTGGCGTTGTACAAGAAGCTGATGGAGATGCC TGTTGGAACCCAGAAGGAAGATTTGAAAAACATGCTCCGAGCATCTGTCTTTCTCATCCGAAGAGCCATA AAGGACATTGTGGCCTCAGTCTTTCAGGCTTCATTGAAATTGCTGAAAATGATAATTACACAGTATATTC CCAAGCACAAGCTGGGCAAACTTGACACAACGCACTGTGTGGAGAGAGCTTTCCCCCTTCTACTCGCCAG AGCTGGAGACTCCTCTGCCCGCCTCCGTGTCATGGCCTTGAATTTCATCCAGGAAATGGCCTTGTTTAAG GAGGTGAAGTCTCTCCAGCTCATTCCGTCCTACTTGGTGCAGCCGCTGAAGGCCAATGCTTCCGTTCATC TGGCCATGAGCCAGGTGGACCTGCTGGCCCGGCTGCTGAGAGACCTGGGCACCGAGAGCTCAGGCTTCAC GGTGGACAATGTGATGAAGTTTGCAGTGAGTGCCCTGGAGCACAGGGTGTATGAGGTGCGAGAGACAGCG GTCAGGATCATCCTGGACATGTACAGACAGCACCCAGCCCTCACCCTGGAGCACCTCCCTCCAGACGACA GCGCCACACGCAGGAACCTTCTCTACAAAGCCATCTTTGAGGGGTTTGCTAAGATAGATGGCTCTGTCTT ACCAACGGAGGCTGAGGTGAGGGCCCAGAAAAGAGTAGCCACCAAAGAAGCAGAAAAGCAAAAGAAAGAA GAGATGAAGGCTCTGCAGGGCCAGTCAGGAGAACTGAGAGAGACCCAGGCTGGAGTCCAGGAGAAGGAGA GTGAGGCTGTGAAGCTCAGGAATCAAGACCCTCAAGGAAGGAAAGCTGTCCTACCCGACACTCCAGAAAT CCCAGCTAACCACTTTTTGGATAATCTGTGTATCTTTTGTGGGGAGAGGAATGAGTCCTTCACAGAAGAG GGCCTGGACCTCCACTACTGGAAACACTGCCTCATGCTGACCAGATGCGACCACTGCAGGCAGGTGGTTG AGATATCCAGCCTTACAGAACACCTGCTGACAGAATGTGACAGAAGGGACGGATTCGGGAAGTGCCCCCG CTGCAGCGAGGCCATCCCCAAGGAGGAACTTCCTGGGCACATCAAAACTAAGGAGTGCAGCCCTGCCAAG CCAGAGAAGGTGGCGAACCACTGTCCCCTGTGTCATGAGAACTTTGCTCCTGGAGAAGAGGCGTGGAAGG TTCATCTGATGGGCCCTGCTGGCTGCACAATGAACCTGCGCAAGACACATGTTCTCTACAAGGCTACAGC CCCGCAGCAAGGGAAAGGCCCAGCAGCAGCAAAGTCAAGCACCTCAGCACCCAAGGTTGGAAGCAAGATC CCCACCCCAAAGGGAGGCCTTAGTAAGAGCTCCAGCAGGACATACATGCGACGGTGACAGAGTTTCCAGT TCTGTCTCTGACAGGCTGTGCAAGGTTCCCCGCTCTAAGCCATGTGTGTTGGCATGGTGATGCAGGGCTC CATGCCCACTGTCCCTTGGCCTCTGGTGTCCCAGTGTCTACTCTCACAGGAACAGTACATGTTATGACCA AAGAGCCCTTTCCAGTGTGGCCCCAGTCCTGGGCCTGTGTCAGTGCTCAGGCCACCACAAGCACAGCACG CACTTCCCCGCTGCTGAGAAGATACACCCCAAACTTGGAGTGCACTGGTGTCTTGCACACATCAGGCTTT GCACAGAGCTCAGCTTTGGTGGCCATAGAAGACCCACGAGCCTGCTGTGCCGCCTCTCATGGTCCTGTCT GCCAGGTTGTCACACCATATATAGCAACAAGCAATAAAGGAAATCGAGCAAAAGACAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:6485859 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6485859 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14300 same embryo
	EMAGE:14297 same embryo
	EMAGE:14296 same embryo
	EMAGE:14299 same embryo
	EMAGE:14298 same embryo
	EurExpress:euxassay_004149 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source