EMAGE mouse gene expression spatial database

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EMAGE:14354

Rab2b RAB2B, member RAS oncogene family ( MGI:1923588)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004144. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004144_01	euxassay_004144_02	euxassay_004144_03	euxassay_004144_04

euxassay_004144_05	euxassay_004144_06	euxassay_004144_07	euxassay_004144_08	euxassay_004144_09

euxassay_004144_10	euxassay_004144_11	euxassay_004144_12	euxassay_004144_13	euxassay_004144_14

euxassay_004144_15	euxassay_004144_16	euxassay_004144_17	euxassay_004144_18	euxassay_004144_19

euxassay_004144_20	euxassay_004144_21	euxassay_004144_22	euxassay_004144_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14354_wholemount_strong.wlz

14354_wholemount_moderate.wlz

14354_wholemount_weak.wlz

14354_wholemount_possible.wlz

14354_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14354_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

telencephalon

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20

spinal cord

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

cervico-thoracic ganglion

weak

regional

weak expression: see section 08

cervical ganglion

weak

regional

weak expression: see section 07 15 16

thoracic ganglion

weak

regional

weak expression: see section 12 14 15

dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5625
Entity Detected:	Rab2b, RAB2B, member RAS oncogene family ( MGI:1923588)
Sequence:	sense strand is shown >T5625 GGCAAATAAGGAGACCCGTAGATTGAGTGGCTAGAGAGGTCGGAGGTAAGTGGCTGTAGAAATCGGGAGG ACCCGGAACACAGACGCCTCGGGACCATGACTTACGCTTATCTCTTCAAGTACATCATCATCGGAGACAC AGGTGTGGGGAAGTCATGTCTCCTCCTTCAGTTTACCGACAAGCGGTTCCAGCCCGTGCACGACCTCACA ATAGGTGTGGAGTTTGGAGCACGTATGGTCAACATCGATGGAAAACAAATCAAACTGCAAATCTGGGATA CGGCTGGGCAAGAGTCCTTCCGTTCTATCACCCGTTCCTACTACAGGGGAGCAGCTGGAGCCCTGCTGGT GTATGACATCACGAGACGCGAAACCTTCAACCACCTGACCTCATGGTTAGAGGACGCCCGCCAGCACTCC AGCTCCAACATGGTTATAATGCTGATCGGGAATAAGAGTGATCTAGAATCTCGAAGGGATGTGAAGAGAG AGGAAGGAGAGGCCTTTGCTCGTGAGCACGGCCTTATATTCATGGAAACATCAGCCAAGACAGCCTGCAA TGTTGAAGAGGCCTACATTAATACAGCCAAAGAAATATATAGGAAGATCCAGCAAGGTTTATTTGATGTC CACAATGAGGCAAATGGCATCAAGATTGGGCCCCAGCAGTCAATTACCTCCTCAGTAGGACCGTGCTCCC CTCAGCAGAACGTTTCTGACATAGGGCCTGACTCTGGCTGCTGCTGAACACCGGGCCTGAGTGAGCGCTT TCTCTTTCCTCTCGGGGAGCAGCTTCAGGTCAGCCAGCGCCACGAAGGAGATCCTCGTTGCTTAGGCTGA GAGCAGCCTCTTTTCAGTGTGCGATGTTTCACCCTTCCACTTAAAACATGAGGAGAAAATTGGGCCCTTT CCGACCTGTCCTCCTTTAGGGACATGAGCATTCCGCGTAGATTATGGCTCTTTCACCCTCTAGTTTCTAA AATGTTAGAATCAAAGTTGATTGTCACAGTAGCTAATAGGCAAACAACTTGCTGTCAACATCTATTTGCT AGGTGTTATGGCACATACCCATAATTTCAGCACTCAGGACTTTTGGCAAAAGGATTGCCAGGAGTTTTGG AGTCAGTCTGGGCTACATAAGTTTCCCTAGGCCATCTGTCTAAAAAAGCCAAAAAGAAAAGAGAGAAAGG AATAAATACATGTGTCTGTTCCTCACCCAATTTTTAGTTGCTAAGGTGAAGGATATTCTGATATAACATT TAGAATTAAGGGTCTGAGTAGCCTTTGGGTGATAGATCTATACATAGTGATTTCATTGCGTGTATTTCCT TGCCTTGGAAACCTGTGTGTCATATGATAAGAGTTCCTATAGAAGCTGAGTGCCTCTGCACTGTCTCGTT GCCAGCTCCTTTTTATTTATTATGTGTGTACAACCTTGAGCATCATTCCTTAGGATCCCCGCACATGGGC CCCACATCTCTAGAGTGCCATGCTTAGGCAAGGCTTGCTGGCCTTTTTCATTGCTAGTTCTTTTCCTTAA CTACAGAACGTAGAAAGCAGTGGAGTGGGTACCGGACCTGTCGCCACACACCCTTACCTAAGAAAACATA AGTTGTTCATTTTGCTTAAAGGACCAGTTCTAGCATTACATTTTCTTTTACCGAATTGCTCTCTCTACTG TTACACACACACACACACACACACACACACACACAGGCACACATGCGAGCCCGTGCACATGCCCTGGAGA TTAAGCTAGGTTCTTAAACATGCTTAGGAAGTGCTGAACCTGAGCTGTGCCCTCAGGAGCCTTCTCTTAC TTACACTTTTTGTTTACAGACAGGTTGCTGAGGCTGGCTTTGAACTTGGGATCCTGAGGCTTCAGACTCC CAGGTGGCTGTTTATTCTCTCTAACCAGCTCTGTTAGATATTTCCTCTAATCAAGCTAATTGGTTACTGA AATGACATATTTATCCCAACTTCTGTACCTTTGCACAAACTCTACCCTCTGACCCTCCACTACCATCATA CTGACACTCTTTTCCTTTAGGAAACCCTCACTGACTGACCTAGGGAGCCCTACGAAGTTGTTAACACAGT ACTTCTTAGCATTCCTACGTTATCCATATGTATATTTATTTGTATGTGTTTCACGTTATAAATGTTTTGT AGTGAACCCAGGGTGGGTGAGGGGGAAGCATCTGTCTCACATAGGCTGTAAGCTCTCTGAGAGCAGGCAC CTGTCATCCCAGTCCATCCCACCGTTCCCTTACTGTACTCTGCAACTCACCATCTGGAATTAAGTGACAT GCTTTAAAACTTAAGAGTTTAGAAGAAACAAAAGGCCTTAGAGTCATCTCTATGTAATTGTATCCTGGTT TCCCCATCTCCTTATCCTGATTTCTCATTGCTCTTCTGTCCTGCCTCACTGTAACTGCCTCCATTCTGTG CAGTCTACAGTAAGAATGCCTGCTTCCAAGACTCAGATACATAGGGACGTTTTTACCCGTTCCGTAGACT CTGTAACTGTCAGCCCAGTTTGCCTCTGCTGACTCCTGGCATCACCTCCTGGGACACTTGCTGTCTTTTT TACTTGTCTCATTTTCTTTCACAGCACTGAGGTCTCACCAAATTTTCTAATTTTTATTGTTAATGACCGT TTTACTATTAAATATATGCATGATCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6492838 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6492838 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14352 same embryo
	EMAGE:14356 same embryo
	EMAGE:14351 same embryo
	EMAGE:14353 same embryo
	EMAGE:14355 same embryo
	EurExpress:euxassay_004144 same experiment
	MGI:4827573 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source