EMAGE mouse gene expression spatial database

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EMAGE:14356

A430033K04Rik RIKEN cDNA A430033K04 gene ( MGI:3583896)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004133. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004133_01	euxassay_004133_02	euxassay_004133_03	euxassay_004133_04

euxassay_004133_05	euxassay_004133_06	euxassay_004133_07	euxassay_004133_08	euxassay_004133_09

euxassay_004133_10	euxassay_004133_11	euxassay_004133_12	euxassay_004133_13	euxassay_004133_14

euxassay_004133_15	euxassay_004133_16	euxassay_004133_17	euxassay_004133_18	euxassay_004133_19

euxassay_004133_20	euxassay_004133_21	euxassay_004133_22	euxassay_004133_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14356_wholemount_strong.wlz

14356_wholemount_moderate.wlz

14356_wholemount_weak.wlz

14356_wholemount_possible.wlz

14356_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14356_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5624
Entity Detected:	A430033K04Rik, RIKEN cDNA A430033K04 gene ( MGI:3583896)
Sequence:	sense strand is shown >T5624 GCTAGGGAAGCTGTTGCTCAGCTTCGGTGGCGCGGTGACGCTGTAAGTGCTGGGCGATGGTGAGAAGACG CCAGCGGCTCCCAGAAGTGGACATGGGGTTGGTGTCATTTGAAGACGTAGCAGTGGACTTCACTTGGCAG GAATGGCAGGAGCTGGATGCTGCTCAGAGGACCCTCTACAGGGACGTGATGCTGGAGAACTATAGGAGCC TGGTGTGGCTGGGGCACTGTTTAGCCAAACCTGAGTTGATCTCCAAGTTGGAAGAAGGATTTGAACCGTG GGGTGTTGCAGAAGCCACAGAGCAGTGCCTACCAGGTGTCCGTAAATGGAGTGCCCCAGTTGAAAAAGGC CAGCAAAGTCAAGAGAAGTATTTGAGGCAAGTCAAAATCATCAAGAAAAATACACCAGATGAAGATAAGG TTGAAGTAGAAAACACATACAATGTGGACTCAAACTGCATTTCAAATATGACTCTGAAGAATGAAGTCTG TTCTAGAGTGTTCTTTCAGGAGCTTGTTAATCCATTGCTAGATGTACCTTTGCCTACTGAGGCTGGCGAG AGGCAGAGTACAGAAGTACCTCATGATCTGAATAGAACTCAGGAAGTTCTCTCATATCCCAAGCATTTCA CTCACCATAGCAAGGATCAGTATTCACAGTGTTGTTTTCAGTACTTTGGACCAGATGAAGCCTTCCACAC AAAAGCAATATTAACACCTGAGATGTTTTATGTTCAGGAAACCTCTAGGACATGCAATAACTATGATAAA TCCTTTGATGAGGTAACAATTCCTGCCCAATATATGACTCAGTTAAGAAAGCAAACTTTGGGATGGAATA TATGTCATAAAATATTCCCTAACAAGACCGAACTTAGCAACCATGACGCTATGCACACTGGAGAGAATGA TGATAAATGTGATTACGAGAAGCCTATCATTAACAAGTCATTATACCTTACAAAACATCAGGAAGCACAT GCAGGGATAGAGCCCCAAGCACACAAAGAAAACATCAAATTCTTCTGCCTAGATGCCGAGCTGCAAACAG TTGATCCAGAACTTCACGGAGAGAAACAAGTTTACGAATGTAAAGTATCAGGGAAAACCTTCCGCCACCA GCCAGAGCACATCAGCCAACAGAGACCACACGCCTGTGAGAGAGCCTGCCAGGCTAAGGAACATGGGGAA GCTGGCTGTGATGAGCCAGCCCTCACCCAACACCAGAGACTTTGTACAGAGGAGAAGGCCTGTGAGGGGA AAGCATGCAGCAAGGCTTTCCACCACAAGTCACTGCTGCCTCAGTATCAGAGCGCTCGCGCAGATGAACA GCAAAGTGACTGCAAAGAATTAATGAAAATTTACTTCTACGTTTCAAGCCCCACGCAGCATCACGGACCT CCTCCTCCTGAGAAACCTTTCAGGTGTAATGATTGTCTGAAGACTTTCTCCCACAAGTCACAGCTCGAGC GACATCAGAGAATGCACACAGGGGAGAAACCCCATGAATGCAAAGAATGCAGGAAAGCCTTCTGTCACAA GTCCCACTTAATCCGGCATCAGGGAATCCATGCGCCTGAGAAACCCTACGAATGTAACGAATGTAAGAAA TCTTTCTACCTGAGGTCACAGCTCACTCTACATGAGAGGACGCACACCGGGGAGAAGCCCTTCGAGTGTA AAGAATGCCGGAAAGCTTTCTCCCGTAATTCACACCTCACTCAACATCAGAAGATTCATACAGGGGAGAA GCCACATAAGTGTAAAGAATGTGGGAATGCGTTTGCCCGCAAGTCACATCTCATTCAGCATCAGAAAACC CACACAGGGGAGAGGCCCTATGAATGTAAAGAGTGCAGGAAAGCTTTCTCTCGTAAGTCCCAACTGATGC AGCACGAGACAACTCACACTGGTGAGAGAGCCTACGAATGCAAAGAATGCAGGAAGACTTTCTATCTCAA GGCGTACCTCACTCGCCACCAGGTAATCCATCAGTCTGAAAAGCCGTTTGAATGTAAGAAATGCGGGAAA GCTTTCTCTCGGAAGTCATACCTCACACGACATCAGAAGATACACAAAGGTCAAACCCTATCTGGGTAAG AAACTTTAGGGATGTAAACGATGGAGAAGGTTCTTAAGCTAGTTGGACTTAACAGGTGAGAAGCCCCATG CGTGTAGGAAAATGTGCTATTGTTACGTCTTGGCTGACATCAGAAATCCCATGCTAGTAATGAACAAATG GAAAAGTGTGAAAGAAAGCTTTGCACAGGAGTTATGTCTCACTATAGGTCAGTGAGTATGCCAGTGAGAA GCCATGGGAAATGCAGGGATGCTGTGTTTAAGTTACACCTCATCATACATTCGTAAATGTATACAAGTGA GAAACCCTGTGACTATAAAGGATGTAGTAGAGCTGCCTGAGAACTTATAACTGCCATTTAAGTTTCAATA ACAAGGGAAATTTTTCAACAAGGGAGACCCCACCGCCTGACAGAAAACTCCCACCGGTGATAAGCCGCGC TCATCTAGGGGAGTTGAGCGCCTGGCAAGTAAGGACTCCCATGAGTGTAAATGATGCTGAGATGTTTCAA GGAGCGCTTGCTTTTCATCTCTGCTCTCTTAGTGTTGCTGCTTACATCTCACTCATACTGAGACAAAGCT AGCCTGGACGACAGAGTAATACACCTGAGGCAAAGCTAGCCTGGACGACAGAGTAATACACCTGAGACAA GGCTAGCCTGGACAATGGAGTTATACACCTATCTTTTTTTTTTTTCTTGAGACTAGATTTCCCTGTGTAG ACCAGGCTAGTTTCAGACTCATGGACACTGACCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCC TCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTGCCTCTG CCTCTGCCTCTGCTCTCCTCACAAGTACTTGGATTAAAGGCATATGCTACCACACCTGGCTTGTAGTAAG ATGTTTTTAATAAAGAAGGTTCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6492709 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6492709 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14352 same embryo
	EMAGE:14351 same embryo
	EMAGE:14353 same embryo
	EMAGE:14355 same embryo
	EMAGE:14354 same embryo
	EurExpress:euxassay_004133 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source