EMAGE mouse gene expression spatial database

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EMAGE:14370

Ralbp1 ralA binding protein 1 ( MGI:108466)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004206. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004206_01	euxassay_004206_02	euxassay_004206_03	euxassay_004206_04

euxassay_004206_05	euxassay_004206_06	euxassay_004206_07	euxassay_004206_08	euxassay_004206_09

euxassay_004206_10	euxassay_004206_11	euxassay_004206_12	euxassay_004206_13	euxassay_004206_14

euxassay_004206_15	euxassay_004206_16	euxassay_004206_17	euxassay_004206_18	euxassay_004206_19

euxassay_004206_20	euxassay_004206_21	euxassay_004206_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14370_wholemount_strong.wlz

14370_wholemount_moderate.wlz

14370_wholemount_weak.wlz

14370_wholemount_possible.wlz

14370_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14370_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7880
Entity Detected:	Ralbp1, ralA binding protein 1 ( MGI:108466)
Sequence:	sense strand is shown >T7880 CGCGTGCGCGCTGGGCCGGCTCGTCCTGTTCTGTCCCAATCATTGTAAACAGGCGAAGGCTGGGCGGGGT GGGAATGGGGCTCCCGCGGCCGGCCCGGGGTGCAGCGCAGGAGGCGGCGGTTGTGGCGGAAGCGTGAGTG CGAGGAGGCGGCGGCGGCTGCAGCGGAGCGGACGCTCGCTCGGCGGCAGGCACTGGTGTAATGGATAGGT AACAGAGAAGACCACTTCCCTTCCTCGGCAGGGCATCAGCATGACTGAGTGCTTCCTGCCCCCGAGCAGC AGCCCTAGTGAACACCGCAGGGCCGAGCATGGCAGCGGGCTGACCCGGACCCCCAGCTCTGAGGAGATCA GTCCTACAAAGTTCCCTGGATTGTACCGGACGGGCGAGCCCTCGCCTCCCCATGACGTCCTCCATGAGCC TCCTGATACAGTGTCAGATGACGACAAAGACCATGGAAAGAAAAAAGGGAAATTTAAGAAAAAGGAGAAA AGGACTGAAGGCTACGCTGCCTTCCAGGAAGACAGCTCTGGAGATGAAGCTGAAAGTCCTTCCAAGGTGA AGAGGTCCAAGGGAATCCATGTGTTCAAAAAGCCCAGTTTCTCTAAAAAGAAGGAAAAAGATTTTAAAAT CAAAGAAAAACCCAAAGAAGAAAAGCATAAAGAAGAAAAGCACAAAGAGGAAAAGCATAAAGAAAAGAAA TCGAAAGACTTGACAGCAGCTGATGTTGTTAAACAGTGGAAGGAAAAGAAGAAAAAGAAAAAGCCAATTC AGGAGCCAGAGGTGCCTCAGATGGACGCACCGAGTGTCAAGCCCATTTTTGGGGTTCCCTTGGTTGACGC AGTAGAGAGGACCATGATGTACGACGGCGTCCGGCTGCCAGCTGTCTTCCGGGAGTGTGTGGACTACATG GAGAAGCACGGCATGAAGTGTGAAGGCGTCTACCGGGTGTCAGGAATCAAATCAAAGGTAGATGAGCTAA AAGCAGCCTATGACCGAGAGGAGTCTCCAAACTTAGAAGAATATGAGCCTAACACTGTAGCCAGCTTGCT GAAGCAGTATTTGCGAGACCTTCCAGAGAATTTGCTTACCAAAGAGCTTATGCCCCGATTTGAAGAGGCT TGTGGGAAGACCACAGAGATGGAGAAAGTGCAGGAATTCCAGCGCTTGCTCCGGGAACTGCCGGAGTGCA ATCATCTTCTGCTTTCCTGGCTCATTGTGCACCTGGACCACGTCATTGCGAAGGAGCTGGAAACGAAGAT GAACATCCAGAACATCTCTATAGTGCTGAGCCCCACCGTGCAGATCAGCAATCGGGTCCTGTACGTGCTT TTCACACATGTGCAAGAGCTCTTTGGCACCGTGGTCCTGAAGCAAGTAACAAGACCTCTGCGCTGGTCCA ACATGGCCACGATGCCCACACTGCCAGAGACCCAAGCAGGCATCAAGGAGGAGATCAGGAGACAGGAGTT CCTTTTGAATTGTTTACATCGAGATCTGCAGGGCGGGATAAAGGACTTATCTAAAGAAGAAAGATTATGG GAAGTACAGAGGATTCTGACTGCCCTCAAGAGAAAGCTGAGGGAAGCTAAAAGACAAGAGTGTGAAACCA AGATTGCACAGGAGATAGCTAGTCTTTCGAAAGAGGATGTTTCCAAAGAAGAAATGAATGAAAACGAAGA AGTTATAAATATCCTTCTTGCCCAGGAGAATGAAATCCTGACAGAACAGGAGGAGCTCCTGGCCATGGAG CAGTTCCTGCGTCGTCAGATCGCCTCAGAGAAAGAAGAGATCGACCGCCTCCGAGCTGAAATTGCGGAGA TCCAGAGCCGTCAGCAGCATGGCCGCAGTGAGACGGAGGAGTACTCGTCCGACAGCGAGAGTGAGAGCGA GGACGAGGAGGAGCTGCAGCTCATCCTGGAGGACCTGCAGCGGCAGAACGAGGAGTTAGAAATAAAGAAC AATCACTTGAACCAAGCAGTCCATGAGGAGCGGGAGGCCATCATCGAGCTCCGAGTGCAGCTCCGGCTGC TCCAGATGCAGCGAGCCAAGTCTGAGCAGCAGCCGCAGGAGGAGGAGGAGCCCGAGAGGCGAGGGGGCAC AGGCCCACCGCCCTGTGACGGTGTCCTCGAGGTGCGGGTAGCCAAGGAGCAGGCGAAGGCCTCGCCCAGC AAAGACCGGAAGGAGACGCCCATCTGAGCTGCGGGCCGGGCCCTGTGCATCTTACTGTGAGTGTGAGTGA GCCGCCTGCCTGCTGGACACTCTGTAAAGGCTCGCTCTCAGAGACGTCCCCTCTCGCACACGTCTGACTC CGTGTCAGGCTCAGGGCCTGGGAGGCTGAAGCAGTGGACACACGGGGGGCTTGCAGGGAACACAGGCATT TCCAGATAGTGTCAGCTTATTTGAAAAATAATTTTCTTTGTTAAAAATAACTATTTTAACCTTTGAGTGG CTTCTTTTTAAACCAAAAATTCTTTTCTTTGGCTTTTTTTTTTTAAATCACAGCAGAATCAGGCTCTCTT TCTTATCAAAGGGTAGGAACCAAATCAGGTCACTTCTGCGCCTGCCTCGTGGACGCTCGTGGTCTCCCCA GCCCTGCAGGGTCTCTGGCCACTCTTGTTTGTGCCTTCCACACCTTTTGGTGCAGATTGTTCAGTGGGGA GCTGCCTCATATTCTCTGACTGGCCAGAAAGGCTCTCGCTGGGGTTCCCTGCACCACGTTGCTTTTCCAC TTCCGAGTTGGGTCTGTCTGTGTGAGTCAGGACGGCCCAGGTTGGTGGCAGACAGAAGTTACCTGTGCAG CCTGCCACCAGCCTGGGCAGTGCAAGCCACTCACTCACTGCCAGGGATCAGCAAAGGCCTCTTCTCTGAG GTGGTCGGAGGTGACCACCTTGACTCTGAGGCGTTGCACAGGAAGCTTCTCAAACCAGAGCCCTTCTCCA CACTGACCTTCCAAGAAAACCACAGTGACTTCAGCTGCTAGCTTGGGCTAGGATACACACCTCTGCCAGG ATAAGCCATGTAAATGCTCTCACGCAGAACGTTCACCTTTCCGCGTCATCTCAAGGGGCTGCTATAAGGA CTCTGACAGAACCGAGCCGAACCGGTAGCTGTGACTGACCCCACCTGGTGTTTGCTTCTAACTCAGATGA CTGCTCCCCGAGTCTGGGAGGCCTGCCTCTTAGGCAGCCAGATCTCACTGTCAGATGAGTGGCCCCCAGG GAGGTCCCCTGTGGCTCTGAAGACAAATCCCACACTCTAAAGTGAGCGACAAACCCGCAGGAGGGCTCTG GTCTCCTCCTAGTTAGTATTTATTTCAGCGCCTGTCTTTTGTGCGGTTTTTTGTTCGCCTCTGCCTATTG CACTGTTGTGACTTGTTGGCCATTATTTGATTTTTGTACGGAAAAAAAGCTTTGTTATAGAAATCAGCAT ACTATTTTTTTAAATCTGGAGAGAAGATATTATGGTGACTGGAAATATGGTCAGGTGTCAAATATAAATG TACAAACGCCTTGCTGCATCCCGTCCGGTGTACAACATCCACTTTATATTTGCTGGCAATATTGAAGAAT TCTTTTTGTTTGTGTAAACTCTAATTTCTATCAAGGTATCATGGGTTTTTAAAATTAGTATTTCATTACA AATGTCTCGATGTTGGTTAACTAATTTTTGCCAGGACCATTATTGATCAAGGAAATAAATTCAACAGCCA TTTGAGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6507517 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6507517 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14375 same embryo
	EMAGE:14371 same embryo
	EMAGE:14372 same embryo
	EMAGE:14373 same embryo
	EMAGE:14374 same embryo
	EMAGE:14369 same embryo
	EurExpress:euxassay_004206 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source