EMAGE mouse gene expression spatial database

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EMAGE:14374

Ints7 integrator complex subunit 7 ( MGI:1924315)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004196. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004196_01	euxassay_004196_02	euxassay_004196_03	euxassay_004196_04

euxassay_004196_05	euxassay_004196_06	euxassay_004196_07	euxassay_004196_08	euxassay_004196_09

euxassay_004196_10	euxassay_004196_11	euxassay_004196_12	euxassay_004196_13	euxassay_004196_14

euxassay_004196_15	euxassay_004196_16	euxassay_004196_17	euxassay_004196_18	euxassay_004196_19

euxassay_004196_20	euxassay_004196_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14374_wholemount_strong.wlz

14374_wholemount_moderate.wlz

14374_wholemount_weak.wlz

14374_wholemount_possible.wlz

14374_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14374_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7877
Entity Detected:	Ints7, integrator complex subunit 7 ( MGI:1924315)
Sequence:	sense strand is shown >T7877 CGCGGCGGGAGAAGATGGCGGTGCTGGGGTAGAGTTTGCAATCTTTGTGACTGGGCGAGTCGGGTAACTG TTCGGGTCATGGCGTCAAACTCAACCAAGTCTTTCCTGGCCGATGCCGGCTATGGCGAACAGGAGCTGGA CGCTAATTCCGCTCTTATGGAACTGGACAAAGGACTGAGATCTGGCAAACTCGGGGAGCAGTGTGAGGCA GTCGTGCGCTTTCCCAGGCTTTTCCAGAAGTACCCGTTCCCTATCCTCATCAACTCAGCATTCCTAAAGT TAGCTGATGTTTTCAGAGTTGGAAACAACTTCCTGCGGCTGTGTGTTCTCAAAGTGACGCAGCAAAGTGA GAAGCACCTGGAGAAGATTCTAAATGTGGATGAGTTTGTGAAGAGGGTGTTCTCAGTGATCCACAGTAAC GACCCCGTGGCCAGAGCCATCACTCTCCGGATGTTGGGAAGTCTGGCGTCCATCATCCCTGAGAGGAAGA ATGCCCATCACAGTATCCGCCAGAGTCTAGATTCCCATGATAATGTAGAAGTCGAAGCTGCTGTTTTTGC AGCCGCAAACTTCTCTGCACAGTCGAAGGATTTTGCTGTGGGGATCTGTAACAAAATCAGTGAAATGATT CAAGGCTTAGCTACACCGGTGGACCTGAAGCTGAAGCTGATCCCGATCCTGCAGCACATGCACCACGATG CCCTCCTGGCCTCCAGCGCCCGCCAGCTCCTGCAGCAGCTGGTCACGTCCTACCCCTCCACCAAGATGGT CATCGTGTCTCTGCACACTTTCACTCTGCTGGCAGCCTCCTCTTTGGTTGACACACCCAAGCAGATTCAG CTTCTGCTGCAGTATTTGAAGAATGACCCCAGGAAAGCCGTGAAGAGACTTGCTGTTCAGGATCTGAAGC TGCTTGCTAGTAAGACACCACACACGTGGAGTAAGGAGAATATTCAGGCACTCTGTGAGTGTGCACTGCA CACTCCTTATGACAGCTTGAAACTCGGGATGTTGTCTGTCCTCTCTACGCTGTCAGGAACCATTGCCATT AAACATTACTTCAGTGTAGTTCCCGGAAATGTAGGCTCTTCCCCCAGATCTTCTGATTTAGTCAAATTAG CTCAAGAATGCTGTTACCATAGCAACAGGGGCATCGCAGCTCATGGAGTGAGAGTGCTAACTAATATAAC AGTGTCTTGTCAAGAAAAAGATCTCTTGTCACTGGAGCAAGATGCTGTCTTCGGCCTGGAATCCCTTCTG GTGCTTTGTAGTCAAGATGACAGTCCAGGTGCTCAATCCACTGTGAAGAGTGCTCTCAGCTGTATGGTGA AGCTGGCCAAGGGCCGGCCCCATCTGAGCAGGTCAGTGGTTGACACTCTGCTAACTCAGCTGCACAGCTC TCAGGATGCTGCCCGGATCCTCATGTGCCACTGCCTGGCAGCCATTGCCATGCAGCTCCCGGTACTGGGC GATGGAATGCTTGGGGACCTCGTGGAGCTCTACAAGGTGATTGGACGATCAGCCACAGACAAGCAACAGG AACTTCTGGTAAGTTTGGCTACTGTGATTTTCGTAGCCAGCCAGAAAGCATTGTCTGCAGAAGTGAAGGC GGTAATCAAGCAGCAGCTTGAAAGCGTCTCCAGTGGGTGGACCGTGTACCGGATTGCCAGGCAGGCTTCC AGAATGGGCAACCACGACATGGCTCGAGAGCTTTACCAGAGTTTGCTGACGCAGGTTGCATCAGAACACT TCTACTTCTGGCTGAACAGTCTGAAGGAGTTCTCCCATGCGGAGCAGTGTCTGACTGGGCTGCAGGAGGA TAGCTTCAGCTCAGCACTTTCCTGCATTGCTGAGTCTTTGAAGTTCTACCACAAAGGGATCGCCTCCCTA ACGGCCGCCAGCACACCGCTGAATCCTTTGAGCTTTCAGTGTGAGTTTGTAAAGCTCCGGATCGATCTCC TCCAAGCCTTCTCGCAGCTCATTTGCACGTGTAACAGCCTGAAGACAAGCCCTCCCCCTGCCATCGCCAC CACCATCGCCATGACCTTAGGAAACGATCTTCAGAGGTGCGGCCGGATCTCCAATCAGATGAAGCAGTCC ATGGAAGAATTCCGAAGCCTTGCTTCCCGGTACAGAGATCTCTATCAGGCATCCTTTGATGCAGATTCGG CCACTCTGAGGAATGTAGAACTCCAGCAGCAGAGCTGCTTACTGATAGCTCATGCCATAGAAGCCCTGGT TTTGGATCCTGAATCAGCAAGCTTCCAGGAGTATGGGTCCACGGGCGCTGCCCACGCCGACAGTGAGTAT GAGAGAAGAATGATGTCCGTGTACAGTCGCGTCCTAGAGGAGGTGGAGTCGCTTAACCGGAAGTATGCCC CAGTTTCCTACATGCACACAGCATGCCTCTGCAATGCCATCATCGCTCTGCTGAAAGTTCCTCTTTCATT TCAGAGATACTTTTTCCAGAAACTACAGTCTACCAGCATCAAGCTTGCTCTGTCACCTTCTCCCCGGAGC CCTGCAGAGCCCATTGCTGTGCAGAATAACCAGCAGCTGGCACTAAAGGTAGAAGGCGTTGTTCAGCACG GATCTAAACCAGGCCTCTTCCGCAGAGTTCAGTCTGTGTGTCTGAATGTTTCCTCCACACTGCAGAGTAA ATCTGGCCAAGACTACAAGATTCCCATTGATAGCATGACCAATGAGATGGAGCAGAGGGTTGAGCCACAT AATGATTACTTCAGCACCCAGTTCCTGTTGAACTTTGCTGTCCTTGGAACGCACAGCATCACAGTGGAGT CATCGGTGAGAGATGCCAATGGCATCGTCTGGAAGACTGGCCCCAGAACTACTATGTTTGTCAAATCCCT GGAAGACCCCTACTCCCAGCAGATTCGCCTACAGCAGCAGGCCCAGCAGCCTTTGCAGCCACAGCCACTG CCGCAGCCGCAGCCTCGCAGTGCCTACACCCGGTTTTAGCTGTGCTACAAGCATACTACTGCCACACTGC ACAGCCATCAGACTACAAGAAGAGTCTTCCGCATGTGCATTAAGTGTCAATGTTACAGTGTGAAATCCAG TATTCACTGATAGCTGTAATGTAACATTCTGGTGAAGTTGTTTTTGTTGTTGTTGTTGTTGTTTTCTTAA AAATTTAGTGTAAGAAATAACCTGGCTCTCAGCCAAAATAAAAGTGCTTTTTTTTTTTTTTTTTTTCGAG ACAGGGTTTCTCTGGGTAGCCCTGGCTGTCCTGGAACTCACTCTGTAGACCAGGCTAGCCTCAAACTCAG AAATTCACCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGCGCCCAAATTTCTTAAACCCAGTCT TCTGTCTCACACTTAGCATTTTTGTTGTATTATTCTTTGTGGTTGGTGAGCTTTTCTTCTTCCAAGAATA GTCACAGATCATTGGTAAATTTGACTGTGTCTCTGTCTACCAGAATTATTGTGCTGACTGGCCCGACCTG GGGTTTTGAGGCAGGCAGGGAGTACTGTTGAGTCGTGCAGTGCTTGTCCTGGGCTGGAGAATGAGGCCTT CACAGAGGCCGCACCTGCCTTCTGGTCTGTGGGAGCCTGTGGGCCACAGGCTCAGCGGCCTGCTGCATTG GCACCTCTTACCCACCTTCTGTCCTGAAGCAATGTTAGTTTATTGTTTTGGTCTTTGATTGACCCAAAAA ACAGCTCCAGCTAGAAAGGTTGGAAATGAATGTGTTGTTTTTTCTGGTATGTGACTAGTGTGGACTTCTG ATATTTTTTTTCTACAAATGGAATGTGGTTCACATATGTCTTCCTGTTTGATGTAAAGGGAACAGATCCT TCCCCGCTCTGGTTTCTACTAGATGACGTATTACTCTACACCTGATGTCTAGACAGCTATCTCCGAGACG TAACCTCAGCTGGGATGCTCAGGGAACAGACTCTGGCTAGCCAGAAAAGCCCTTTTTCTACTTGAGTCAT GGTCAGAAACTACATGACAACACACTGATTCTAGGTTTGATTTAACAGTTGACTATGGCTGCTCAGTGCT TTTGGACCACGGTTAATATGATGAGCTGAGCCTGCCGATACCCCCTGTGGTCATTGGTGTTGGGTGCAGT GGGCTGCTAAGGGCATGAGCCCTGGATTTCCCTGTGTAAATAAATCTCCTAATTTCTTTAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6506658 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6506658 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14375 same embryo
	EMAGE:14371 same embryo
	EMAGE:14372 same embryo
	EMAGE:14373 same embryo
	EMAGE:14369 same embryo
	EMAGE:14370 same embryo
	EurExpress:euxassay_004196 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source