EMAGE mouse gene expression spatial database

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EMAGE:14389

Slc7a4 solute carrier family 7 (cationic amino acid transporter, y+ system), member 4 ( MGI:2146512)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004221. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004221_01	euxassay_004221_02	euxassay_004221_03	euxassay_004221_04

euxassay_004221_05	euxassay_004221_06	euxassay_004221_07	euxassay_004221_08	euxassay_004221_09

euxassay_004221_10	euxassay_004221_11	euxassay_004221_12	euxassay_004221_13	euxassay_004221_14

euxassay_004221_15	euxassay_004221_16	euxassay_004221_17	euxassay_004221_18	euxassay_004221_19

euxassay_004221_20	euxassay_004221_21	euxassay_004221_22	euxassay_004221_23	euxassay_004221_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14389_wholemount_strong.wlz

14389_wholemount_moderate.wlz

14389_wholemount_weak.wlz

14389_wholemount_possible.wlz

14389_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14389_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 17

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 15 16 17 18 19 20

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17

dorsal root ganglion

weak

regional

weak expression: see section 07 08 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4367
Entity Detected:	Slc7a4, solute carrier family 7 (cationic amino acid transporter, y+ system), member 4 ( MGI:2146512)
Sequence:	sense strand is shown >T4367 CAGGTCCGCTGCAGGTCTGCTTCTCTCTGCCAGTTCCACTGCCCTGAAAGCTGACAGGAAGGACTTCCAT GCGTTCTATCCCTCTGCCCACAGTCCCTGACACGCGTCCCATTCAAGCCTTCTAAAGTTCCAGAAACTGG TTCAGTACTCCGTCTGCCATGGCCCGGGGACTGCCCAGTACTGCCTGTTTGGCACGCTTCTGCCAGAAAC TGAACCGTCTGAAGCCACTGGAAGAGTCTAGCATGGAGACATCACTGCGGCGCTGCCTGTCCACACTGGA CTTGACTCTACTGGGTGTGGGTGGCATGGTGGGATCTGGGCTCTATGTGCTCACAGGCACAGTGGCCAAG GACATGGCTGGCCCCGCTGTGCTCTTGTCCTTTTTGGTGGCCGCTGTAGCCTCCCTGCTGGCGGCCCTAT GCTATGCAGAGTTTGGAGCCCGTGTGCCCCGTACTGGCTCAGCATACCTATTCACGTACGTGTCCATGGG GGAGATATGGGCATTCCTCATAGGCTGGAATGTGCTTCTAGAATACCTCATTGGAGGTGCCGCTGTAGCC CGTGCCTGGAGTGGCTATTTGGATGCCATCTTTAACCACAGCATTCGCAACTTCACGGAGTCTCACCTGG GCGTCTGGCAGGTGCCCTTCCTAGCTCACTATCCAGATTTTCTGGCCGCTGGCATTTTACTCGTGGCTTC TGCCTTTGTCTCCTGCGGAGCCCGAGTCTCCTCCTGGCTTAATCACACGTTCTCAGCCATCAGTCTGATT GTGATCCTCTTCATCATTGTCCTGGGTTTCATCCTGGCCCGTCCTCACAACTGGAGTGCAGAAGAAGGTG GTTTCGCACCCTTCGGCTTCTCTGGCATCCTGGCTGGCACAGCCACCTGTTTCTATGCCTTCGTGGGATT TGATGTTATTGCTGCCTCCAGTGAGGAGGCCAAAAACCCACGGTGGGCGGTGCCCATGGCCATCGCCATC TCCCTCAGCCTGGCAGCTGGTGCCTATATTCTGGTCTCCACTGTGTTAACCCTCATGGTACCTTGGCACA GCCTAGACCCTGACTCGGCGCTTGCTGATGCTTTCTACAGGCGGGGTTACAGCTGGGCTGGCTTCATTGT GGCAGTTGGCTCTATCTGTGCCATGAACACCGTCCTGCTCAGCAACCTCTTCTCCCTGCCGCGCATTGTC TACGCCATGGCTGCCGATGGGCTCTTCTTCCAGGTGTTTGCCCGTGTACACCCCCGGACACAGCCTCCTG GTCCTGGGGGCTCATCAGCAGCAAAAGAAGCAAGACACTTTCCAGATCCCTTTGGTGCCCCTGACTCCAG CCCTGAGCATCCTTCTCAACACTTGCCTCATGCTGAAGCTGAGCTACCTGACCTGGCTACGCTTTATCTT CTGGCTGCTGGTCGGACTTGTCGTGTATTTTGGCTATGGCATCTGGCACAGCAAGGAGAACCAGAGGGAG CCATTGGAGCTGACTACAGCACACTACGTGGTATTCCCCAGCGGTAGCCTGGAGGAAACTGTGCAAGCTG TGCAGCCCTCTAGTCAATCACCAGTCCGGGAGTCAGGTTGTACTGAGTAGCTGGCCACGGAGGCTTGCTC ATGGAGGTCTCCATCCCCAACATCCAGAGGCCAGAAGAATTGGCAGCTGCCTACTTAAGGAAGCTTGGGT TTGAAGCCTGCCCAGACTGGGCTGGGAGTGTTCTCAGAGTCTCAACACAGGAACGGAGTCTTCGGCTATC ATGGACTAGGCTGAGGCTAGTGCTGGTGTGGACTCGTTGCCCACTGTCTTCCTGTTTATGATTAACTCTG GATACCTGGGCAGGTGGGGCAGAGTGGGCAGGGAAGTGGCTTCAGCCCCTGAGACCCAGAATAATAACTA ATCAGCTAGTGTTGTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4911158 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4911158 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14386 same embryo
	EMAGE:14387 same embryo
	EMAGE:14390 same embryo
	EMAGE:14388 same embryo
	EurExpress:euxassay_004221 same experiment
	MGI:4828277 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source