EMAGE mouse gene expression spatial database

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EMAGE:14394

Pcdhga4 protocadherin gamma subfamily A, 4 ( MGI:1935216)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005624. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005624_01	euxassay_005624_02	euxassay_005624_03	euxassay_005624_04

euxassay_005624_05	euxassay_005624_06	euxassay_005624_07	euxassay_005624_08	euxassay_005624_09

euxassay_005624_10	euxassay_005624_11	euxassay_005624_12	euxassay_005624_13	euxassay_005624_14

euxassay_005624_15	euxassay_005624_16	euxassay_005624_17	euxassay_005624_18	euxassay_005624_19

euxassay_005624_20	euxassay_005624_21	euxassay_005624_22	euxassay_005624_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14394_wholemount_strong.wlz

14394_wholemount_moderate.wlz

14394_wholemount_weak.wlz

14394_wholemount_possible.wlz

14394_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14394_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7886
Entity Detected:	Pcdhga4, protocadherin gamma subfamily A, 4 ( MGI:1935216)
Sequence:	sense strand is shown >T7886 CTGGACTCCAGGAGCACCAGCGTCCAACAGTACAAGAACAGTATCTTGAAACCCAAAGATTGTGGGGTTC ATCAGGCCTGCACAGACTGAACGCCCAAGCTCAGGACCCTCTGCGGCAGGACTGCAATGGCGGCTCCTTA CAAATCAGACCGCAGGGGACTAATCTGGATCTGCATTTTCCTTGGATCCCTATGCGACATCCGCGCGGAA CAGATCCGGTACTCTGTGCCGGAGGAGCTGGAAAGGGGCTCTGTGGTGGGTAATCTCGCCGCGGACCTGG GGCTGGAGCCTGGAAAACTGGCAGAACGCGGAGTCCGCATCGTCTCCAGAGGTAAGACCCAGCTCTTTGC TCTGAACCCGCGAAGCGGCAGTTTGGTGACAGCAGGGAGGGTCGACAGAGAAGGGCTCTGTGACAGATCC CCAAAGTGTACCGCAAATCTGGAGATTCTCCTAGAGGATAAAGTGAGGATTCTAGCCATAGAAGTGGAGA TTATCGATGTTAATGATAATGCCCCCAGCTTTGGAGCACAGCAGAGAGAAATAAAAGTCGCTGAAAGTGA AAATCCTGGGACAAGATTTCCTCTTCCTGAAGCCTTTGATCCGGACATAGGGGTGAACGCCTTGCAGGGT TACCAGCTCAGCTCCAATGATCACTTCTCCCTGGATGTGCAAAGTGGACCGGATGGGATTAAGTATCCAG AGCTAGTGCTGGAAAATGCCTTAGACCGCGAGGAAGAAGCCGTTCATCACCTGGTTCTCACCGCCTTCGA CGGAGGCGACCCGGTTCGCTCTGGTACTGCCACAATTCAAGTAACACTCGTGGACACCAACGACAATGCT CCTGTATTCACTCAGCCAGAGTACCACATTAGTGTGAAAGAGAATTTGCCTGTAGGCACCCGGCTGCTTA CCATAAAAGCCACTGACCCGGATGAAGGAGTCAACGGAGAAGTGACGTATTCTTTCCGGAACGTAAGAGA GAAAATCTCCCAGCTCTTCCAGCTGAATTCTCTGACTGGGGACATTACAGTGTTGGGGGAACTGGATTAT GAAGACTCTGGATTCTACGATGTTGACGTAGAAGCCCATGATGGGCCTGGTCTTCGAGCCAGAAGTAAGG TACTGGTGACAGTTCTGGATGTAAATGACAATGCCCCGGAAGTCACAGTCACCTCTCTCACCAGCTCTAT CCAGGAAGCTTCTTCCCCAGGCACAGTGATTGCACTCTTCAACGTGCACGACAGCGACTCTGGAGAGAAC GGCCTTGTCACTTGTTCTATTCCAGATAATCTTCCATTCAGACTGGAAAAGACCTATGGAAATTATCATC GGTTGTTGATACACAGGACTCTGGACAGGGAAGAAGTCTCTGACTATAACATTACAATAACTGCCACCGA CCAGGGAACTCCACCTCTGTCTACAGAGACTTATATCTCATTGCAGGTGGTAGATATTAATGACAACCCG CCGACCTTCACCCATGCCTCCTATTCTGCTTACATTCCAGAAAACAACCCTAGAGGAGCTTCCATCTTAT CCATAACAGCCCAGGACCCAGACAGTGGTGAGAATGCCCAGGTTATCTATTCTCTGTCAGAGGACACTAT CCAGGGGGCACCTATGTCCTCCTACGTCTCCATAAACTCCAATACCGGTGTCCTTTATGCACTGCGTTCC TTTGATTATGAGCAGTTTCAAGACTTGAAGTTACTGGTGACAGCCAGGGACAGTGGGACTCCTCCACTCA GCAGCAACGTGTCCCTGAGCTTGTCTGTGCTGGACCAGAACGATAACACCCCTGAGATCCTGTATCCCAC CATCCCCACTGATGGTTCTACTGGTGTGGAGCTGACACCTCGTTCTGCAGATCCTGGATACCTGGTGACC AAGGTGGTGGCCGTAGACAAAGACTCAGGACAGAACGCCTGGCTGTCCTACCGCCTGCTCAAGGCCAGCG AGCCAGGGCTCTTCTCAGTGGGGCTGCACACAGGTGAGGTACGCACTGCTCGGGCCCTGCTGGACAGAGA TGCTCTCAAGCAGAGCCTGGTGGTGACCGTGCAGGACCATGGCCAGCCCCCTCTCTCCGCCACTGTCACA CTCACCATAGCTGTGTCAGACAACATTCCAGACATCCTGGCTGATCTGGTCAACATTAACGCCCCCATTG ACCAGGAGGATTCAGACATCACATTGTACCTGGTGGTAGCTGTGGCTGCGGTCTCTTGTGTCTTCTTAGC CTTTGTCATTGTGCTGCTGATACACAGGCTCCGTCGCTGGCACTCAACTCGCCTGCTTCAGGCTGCAGGT AATGGGTTGTCATCCTTGCCTGCCTCACATTTTGTGGGTGTGGATGGTGTACACGCTTTCCTACAGACCT ATTCTCATGAGGTCTCACTCACAGCAGACTCTGGGAAGAGTCACCTGATCTTCCCACAGCCCAACTACGC AGACACACTCATCAGCCAGGAGAGCTGTGGGAAGAGTGATCCTCTCCTGGTATCTCAAGACTTGCTTGAA ATAAAAGGAGACTCCAGTCTGCAGCAAGCCCCGCCCAACACTGACTGGCGTTTCTCTCAAGCCCAGAGAC CCGGCACGAGCGGATCCCAAAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTTGATACAGAGAT GCTGCAAGCCATGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGGGAGGGGGCGCT GGCACCATGGGTCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGACTACCGCCAGA ACGTGTACATCCCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAAACGAGATGGCAAGGCTCCAGC AGGTGGTAATGGCAACAAGAAGAAATCGGGCAAGAAAGAGAAGAAGTAATATGGAGGCCAGGCCTTGAAC CACAAGGCAGCCTCCCTCCCCAGCCAGTCCAGCTCGTCCTTACTTGTACCCAGGCCTCAGAATTTCAGGG CTCACCCCAGGATACTGGTAGGAGCCAGGGCCATGCTCCCCGTTGGGAAACAGAAACACGTGCCCAAGCC AACACCCCCTCTCTATACCCTAGGGAGTTGAATATGCAAAGAGAGTTCTGCTGGGACCCCCCTATTCAAT CAGTGATTGTACCCACATGGGTAGCAGGGTTTGTGTGTGTGGACATACACACACACACACACACACACAC ACACACAAACCATGCCACACTCCCTTAGTTACAGCTGAACTCCTCCATCTTCCAACCCAGGCAGGCTTAT CCATCTCCTGCCGCCCTCTCCCCACCCCACCCACTCCTTCAGTCCCTCTCTTTGAGCAAGGTAGCTGGGC TGTTGGGGTGACCGGTGAGCTAAGCCGGCTCCTAGTTCTGAACGGTAGGGAGAGTATTACAATCTTTTGG CCTCTCCTTCAGTTCAGCTTTCCCCCAAAGCATGGTTTGGTACCAGTCTTCTCTTTTCCTTCCAGAGCCT GAGACCAATGCTTAAGTTTTGGGGGAACAGGCACCCTCCCTCTGGTACTGATGATGCTTGCTGGATTTTG GGAAGGCATTTTGCTACTAAGCCTCTTTTCAATGCCTGGGGACAACTAGTCTTTTGTTTTTCATTGTTTG ATGTTCCCACTGCATGCTGTGACTTCCCTACACCTCAAAGAAGAGACTCCTTTGCATGTCTGGAGACAGT AGGGGGTGGTAAACTAAAGGGAAGTTGAGAGTGTATACTGCTGGGAGTGGGGCAGTGGACAAAACAGCCT GTTAATAGGGTCTTGCAAGGGGCTCTGTATGTACCCAGGGGACTGGCTCAATCCTCACATTCTAGCCATA GACAAACACCAGGCCAGACCCCTCCATTCTGGTTCAGCCTGGGCAGCTTGGGCTGAGCCACCAGGACCAA TGGATTTAAGCTGACATTTCAGTCCAAGACGACGACTTCTAAGTGAGTTTAAGACCAGAGAGGAAAGAGG GGCCTCTGTGGGTGCTGGGTACTCCAGAGGTGCCCTTGGTGGGAGGACCAGTGGTCTTAGCAGGAAGGGG GGCCCAGCAAGGTCATTCTTGGACCCTGGGTCTAGTCCAGTAGCTAGAAAAAGGGACCAAGTGGCCATAA AGTCCCAGCCAATGATGGGGCTTTTCCAGTGGGCCCCTGTAGACCTCAAGCCCCTGGCCTCCACCTTACC AGGTGCCATTTCTTCTCAGAAGGCCACTGCCCAGGCCCCCAGGCCGCCCCCTTGTGGCCAGAGAACGGTT AAAGCCCCCCAGTGCCTCCTTGTGCATAGAACTTCCTCTGCCCTAGCCCTTACAGTAGTGTAGAAGATCC CCCTCCCCCGCTGGTGTAGAATAGCCAATAGTATAGTGTGGTGTGCTTTTACGTGATGGCGAGTGGGCAG CGGGCGGTGGGCTGTACACAGCCGTCTGTCCTTTGAATCTCAATCTGCCTGCGCGGCCCGTGCTGTGTTT TGTGCTGTGTCCACACGCTGCGGCGACCCCTCCCTGTACTGACTTCTCTATAAAAAGCTTCTCTTCGCAT AGTCACGTAGCTCCTGCCCATCCCCCTTCCGATATCTCTCGCAAGTTTTATACTCTAATATTTATATGGC TTTTTTCTTCGAAAATTAAAAATAAAAAAATGGTTTCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6508140 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6508140 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14393 same embryo
	EMAGE:14391 same embryo
	EMAGE:14395 same embryo
	EMAGE:14392 same embryo
	EurExpress:euxassay_005624 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source