EMAGE mouse gene expression spatial database

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EMAGE:14403

Uimc1 ubiquitin interaction motif containing 1 ( MGI:103185)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004273. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004273_01	euxassay_004273_02	euxassay_004273_03	euxassay_004273_04

euxassay_004273_05	euxassay_004273_06	euxassay_004273_07	euxassay_004273_08	euxassay_004273_09

euxassay_004273_10	euxassay_004273_11	euxassay_004273_12	euxassay_004273_13	euxassay_004273_14

euxassay_004273_15	euxassay_004273_16	euxassay_004273_17	euxassay_004273_18	euxassay_004273_19

euxassay_004273_20	euxassay_004273_21	euxassay_004273_22	euxassay_004273_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14403_wholemount_strong.wlz

14403_wholemount_moderate.wlz

14403_wholemount_weak.wlz

14403_wholemount_possible.wlz

14403_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14403_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7203
Entity Detected:	Uimc1, ubiquitin interaction motif containing 1 ( MGI:103185)
Sequence:	sense strand is shown >T7203 GCCGCTCGCGACCCCCAGCGTTCAGGAGGCGCTGGGCGGCGCTGCCAGATCCGCGGTAAAAGGATGCCAC GAAGGAAGAAAAAAATTAAAGAGGCCTCTGAAAGCCAGAACTTGGAGAAGAAGGATCTGGAAACAAGCAG TTGTGTCAGTATAAAGAAGAAGCGTAGACTTGAGGACTTACTCATAGTGATATCCGATAGCGATGGAGAG GAAACAAAAGAGGAGAATGGATTGCAGAAAACGAAGACAAAACAGTCGAACAGATCAAAGTGTTTGGCTA AAAGAAAAGTTGCACACATGTCAGAAGAAGAACAATTTGCTTTGGCTCTCAAGATGAGTGAGCAGGAAGC TAGGGAGGTGAATAACCAGGAGGAGAAAGAAGAGGAGCTCTTGCGGAAAGCCATTGCTGAAAGCCTGAAT AGTTGCTGGTCTTCTGCTGCTTCTGCTACCAGATCTCGACCTCTGGCTGCTGAACTATCTTCACATTCCC ATCAAGAGAACACCAAAGACTCTGGGACCACTGAAGGCGTATGGCAGCTGGTACCTCCATCACTGTGTAA AGGCTCACATGTCAGTCAGGGAAACGAGGCTGAGCAAAGAAAGGAGCCCTGGGACCACAATGAAAACACT GAAGAGGAGCCGGTCTCTGGCAGCTCAGGAAGCTGGGACCAGTCAAGCCAGCCAGTGTTTGAGAATGAGA ACGTTAAATGTTTTGACAGATGTACTGGCCACTTGGCTGAGCACACACAGTGTGGGAAGCCACAGGAAAG TACTGGGAGTGGTTATGCTTTTTCCAAAGCTGTCCAGGGTAGGGGGGACACGTCTAGGCAATGCCTTCCT ATCCCAGCAGACACAAAAGGTCTCCAGGACACTGGGGGCACTGTGCACTACTACTGGGGTATTCCATTCT GCCCTGCTGGAGTAGATCCCAATCAATACACCAATGTCATTCTCTGCCAGTTAGAGGTTTATCAGAAGAG CCTGAAAATGGCTCAGAGACAGCTTGTTAAAAAAAGAGGGTTTGGGGAACCAGTGTTACCTAGACCTCCT TTTCTGATCCAGAATGAATGTGGCCAAGAAGATCAGACTAGTGACAAAAATGAAGGCATCTCAGAAGATA TGGGAGATGAAGCCAAAGAGGAAAGGCAGGAATCTAGGGCATCTGTCTGGCACTCAGAAACCAAGGATTT TCAAAAAAGTCCAATTAAAAGCTTGAAACAGAAACTTTTGTTGGAGGAAGAACCAACAACCAGTCGTGGT CAGTCTTCCCAAGGTCTGTTTGTTGAAGAAACCTCTGAAGAAGGTCTGAAGAGTTCTGAAGGAGACAACT CTGTGCCCACCACGCAAAGCATTGCAGCTTTGACCAGTAAGAGAAGTTTAGTTCTTATGCCGGAAAGTTC TGCAGAAGAAATCACTGTTTGCCCTGAGACACAGTTAAGTTTCCTTGAACCCCTTGACCTCAATAGAGAA GACTCTCCAGATAGCAGAGAGCTCCCCATTGAAGTAAGGATGGCAGTGGGCGATAAGCAGGTTGCTAATA GGGAAGATTGTATGAAGGAAAACCCTCCTCCTGCAGTCTCATCTAGTACCCGGGTATCCTGCCCACTGTG TAACCAAGACTTTCCTCCCACAAAGATTGAACAGCATGCCATGTACTGCAATGGTCTGATGGAGCAGGAA ACAGTGTTGACTCGGAGACGAAGAGAGGCCAAGAACAAGAGTGGCGGTCGGACAGCTGCACAGCCGGCTC TGGATGCCAACAGGAAGGAGAAGTGTTATCTATGTAAGTCCCTGGTTCCACTTGGGGAGTATCAGTGCCA TGTGGAGGCCTGTCTCCAGCTTGCAAAGGTTGACAGAGAAGATGGGATTGAAGGGACAAGGAGACCAAGG GTGTGTGCACCTGTGGAGGGGAAACAACAGCAGCGGCTGAAGAAGTCAAAGGACAAAGGCCATAGTCAAG GCCGACTCCTCAGTCTCTTGGAGCAGTCTGAGCATAGGACCACAGGTGTAGAGAAAAAACCCAAGTATTC GGAAGTAAGAACCTTCAGGATGCCCTCACCAGAGGTGGAAGAGGCTAGCTGCAGCAGAGAGATGCAGAGT ACCCTCTCACAGCTCAACTTAAATGAGTCTCCCATCAAGTCTTTTGTTCCTGTTTCAGAAGCTACAAATT GCTTAGTGGACTTTAAAGAACAGTTTGCTTTCCGGTCACGAACTAAATCAGGCAGAGGAAGGAGGAGAAA ATCTTGAATTTCTTGAGACTGGAAGTTGACCAGAACACACATCGTTGGGTTGATCGTGTTCATTAAGTAT AGTGGTCTCTAGTTTGTTGTGAGAGTTCTGACCCTGTTGTTATCACCACCAGCACCCATTCAGTATCCTG GCTTTATATTTTATAAGATCAGTTCAGACAACTGTGAATATTATTCTGTTTGAATTTGCTTATAGTTAAA ATTTAAATATATTTATCTTTGTATGAAATCTTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4038778 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4038778 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14406 same embryo
	EMAGE:14404 same embryo
	EMAGE:14405 same embryo
	EMAGE:14402 same embryo
	EurExpress:euxassay_004273 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source