EMAGE mouse gene expression spatial database

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EMAGE:14405

Psen2 presenilin 2 ( MGI:109284)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004270. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004270_01	euxassay_004270_02	euxassay_004270_03	euxassay_004270_04

euxassay_004270_05	euxassay_004270_06	euxassay_004270_07	euxassay_004270_08	euxassay_004270_09

euxassay_004270_10	euxassay_004270_11	euxassay_004270_12	euxassay_004270_13	euxassay_004270_14

euxassay_004270_15	euxassay_004270_16	euxassay_004270_17	euxassay_004270_18	euxassay_004270_19

euxassay_004270_20	euxassay_004270_21	euxassay_004270_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14405_wholemount_strong.wlz

14405_wholemount_moderate.wlz

14405_wholemount_weak.wlz

14405_wholemount_possible.wlz

14405_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14405_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7204
Entity Detected:	Psen2, presenilin 2 ( MGI:109284)
Sequence:	sense strand is shown >T7204 CCACGCGTCCGCCCACGCGTCCGGGCGGAGCAGACCATGCTCGCATTCATGGCCTCTGACAGCGAGGAAG AGGTGTGTGATGAGCGGACGTCCTTGATGTCAGCCGAGAGCCCCACATCTCGCTCCTGCCAGGAAGGCAG GCCAGGCCCGGAGGATGGAGAGAGCACTGCCCAGTGGAGGACTCAGGAGAGCGAAGAAGACTGTGAAGAG GACCCGGACCGCTACGCATGCAGTGGGGCTCCTGGGCGACCGTCGGGCCTGGAGGAAGAGCTGACCCTCA AGTATGGGGCGAAGCATGTGATCATGCTATTCGTGCCTGTCACGCTGTGTATGATCGTGGTGGTGGCCAC TATCAAGTCTGTGCGTTTCTACACTGAGAAGAACGGGCAGCTCATCTACACGCCCTTCACGGAGGACACG CCCTCGGTGGGCCAGCGGCTCCTCAACTCCGTGCTTAACACCCTCATCATGATCAGCGTCATCGTAGTCA TGACCATCTTCCTCGTGGTACTCTACAAGTATCGATGCTACAAGTTCATCCATGGCTGGCTGATCATGTC CTCCCTGATGCTCCTCTTCTTGTTCACCTACATCTACCTCGGGGAAGTGCTCAAGACCTACAATGTGGCC ATGGACTATCCCACACTCTTCCTGGCTGTCTGGAACTTCGGGGCAGTGGGCATGGTGTGCATCCACTGGA AGGGGCCTCTGGTGCTGCAGCAGGCTTACCTTATTGTGATCAGCGCACTCATGGCCCTGGTGTTCATCAA GTACCTGCCGGAGTGGTCTGCCTGGGTCATCTTGGGTGCCATCTCTGTGTACGATCTCGTGGCCGTGCTG TGCCCCAAAGGGCCACTGAGGATGCTGGTGGAAACTGCCCAGGAGAGAAATGAGCCCATATTTCCTGCCC TGATATACTCATCTGCCATGGTGTGGACGGTGGGCATGGCAAAGCTGGACCCCTCCTCTCAAGGAGCGCT GCAGCTCCCCTATGACCCAGAGATGGAAGAAGACTCCTACGACAGTTTTGGAGAACCCTCATACCCTGAA GCCTTCGAAGCTCCCCTGCCTGGCTACCCAGGGGAGGAGCTGGAGGAGGAGGAGGAAAGGGGCGTGAAGC TCGGCCTGGGAGACTTCATCTTCTACAGCGTCCTGGTGGGCAAGGCTGCAGCCACTGGCAACGGAGACTG GAACACTACGCTGGCCTGTTTTATCGCCATCCTCATTGGCTTGTGTCTCACCCTCCTGCTGCTTGCTGTG TTCAAGAAGGCTCTGCCCGCCCTCCCCATCTCCATCACCTTTGGACTCATCTTCTACTTCTCCACAGACA ACCTGGTGCGCCCTTTCATGGACACTCTGGCCTCCCACCAGCTCTACATCTGACAGGAGCCAGGCGGAGG CTCCGAGAGCTATGGGGGCGTCACTGGATGCAGGTGTATAGTTTTACACTCTAGTGCCATATATTTTTTA AAGAAATTTTCTTTCCTTACAAAAGAAGAAGAAGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4038761 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4038761 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14406 same embryo
	EMAGE:14404 same embryo
	EMAGE:14402 same embryo
	EMAGE:14403 same embryo
	EurExpress:euxassay_004270 same experiment
	MGI:4827468 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source