EMAGE mouse gene expression spatial database

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EMAGE:14451

Tasp1 taspase, threonine aspartase 1 ( MGI:1923062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004259. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004259_01	euxassay_004259_02	euxassay_004259_03	euxassay_004259_04

euxassay_004259_05	euxassay_004259_06	euxassay_004259_07	euxassay_004259_08	euxassay_004259_09

euxassay_004259_10	euxassay_004259_11	euxassay_004259_12	euxassay_004259_13	euxassay_004259_14

euxassay_004259_15	euxassay_004259_16	euxassay_004259_17	euxassay_004259_18	euxassay_004259_19

euxassay_004259_20	euxassay_004259_21	euxassay_004259_22	euxassay_004259_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14451_wholemount_strong.wlz

14451_wholemount_moderate.wlz

14451_wholemount_weak.wlz

14451_wholemount_possible.wlz

14451_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14451_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7180
Entity Detected:	Tasp1, taspase, threonine aspartase 1 ( MGI:1923062)
Sequence:	sense strand is shown >T7180 CGACTACTTCCTGTGTGTGAGTGCTCTGCGCTGACGCGGGTTGACTCCCTTTGCACAGTTGGATGGAAGG ATGCTCTTTTCCTGATAGAAGATGATGATCTTGGAGAATGATCATGGAGAAGGGGATGAATTCTGGAGAA GGACTGCCTTCCAGATCATCTCAGGCATCTGCTGCTAAAGTAACAGTCAAGGAGTTAGAAACACAGCAGC CCTGTAAGGAGAAACGAGGGGGCTTTGTGCTGGTGCATGCAGGTGCAGGCTATCATTCTGAATCCAAAGC CAAGGAATATAAACATGTATGCAAACGAGCTTGTCAGAAGGCAATTGAGAAGCTACAGGCTGGTGCTCTT GCTACAGATGCAGTGGCTGCTGCTCTGGTGGAACTTGAGGATTCTCCTTTTACAAATGCAGGAATAGGAT CTAATCTAAATCTCTTAGGAGAGATTGAATGTGATGCCAGCATAATGGATGGAAAGTCCTTAAACTTTGG AGCCGTTGGAGCACTGAGTGGAATCAAGAATCCAGTCTCAGTTGCTCACAGGCTCTTGTGTGAAGGGCAG AAGGGCAAGCTCTCTGCAGGCAGAATTCCTCCCTGCTTTTTAGTTGGAGAAGGAGCCTACAGATGGGCAG TGGATCATGGGATACCCTCTTGCCCTCCTAGCACCATGACCACAAGATTCAGCTTAGCTGCATTTAAAAG GAACAAGAGGAAACTAGAGCTGGCAGAAAGGGTGGAAACAGACTTCATCCAGCTGAAGAGAAGACGACAA TCAAGCGCAAAGGAAAATGACTCAGGCACTTTGGACACGGTGGGTGCTGTGGTCGTGGATCACGAAGGGA ATGTTGCTGCTGCTGTCTCCAGTGGGGGCTTGGCCTTGAAACATCCAGGGAGAGTTGGGCAGGCTGCTCT TTATGGATGTGGCTGCTGGGCTGAGAATACTGGCGCTCAGAACCCCTACTCCACAGCTGTGAGTACCTCA GGATGTGGCGAGCATCTTGTGCGCACCATACTGGCTAGAGAATGTTCACACGCTTTACAAGCTGAAGATG CTCACCAAGCTCTGTTGGAGACTATGCAAAACAAGTTTATCAGTTCACCTTTCCTTGCCTGTGAAGATGG CGTGCTCGGTGGAGTGATCGTTCTCCGGTCATGCAGATGTTCTTCAGAGTCTGACTCCTCCCAAGATAAA CAGACACTGCTTGTGGAATTTCTGTGGAGCCATACTACAGAGAGCATGTGCGTGGGATATATGTCAGCCC AGGACGGCAAAGCCAAGACTCACATTTCACGACTCCCTCCTGGTGCTGTGGCAGGACAGTCTGTTGCCAT CGAAGGTGGGGTTTGCCGCCTGGAGAGCCCCGTGAACTGACTCCAGGCTGAATATGAAGTGTCTGAGAGG CATTTCAGAGCCTGAGCTTTGGGCTGTTTAATTGAAGTTGGGTGTTTTAATCTTCTTGTTTTGTAATTTT TATTGCAACCTCGGGCACTGCTTGGGACACAAGTGCTGCTATACTTAGTGCTGGTTGACACTCGAGTCTT AGATGGGTGAGCGGAAGTCTGTGTGGGTGTGTATCCGTGTGCAGTGTGTGCGCTTGCTTCCCCCGGATGG GATGAGTTCTCCTTAAGTGCAACCTATGAATGAGAATGATGGAATCTTTACAAAATGTGTGCTTTAACTG TTTACAAGGAAACCTAAAGTTGTAGAAAACTTTTATTTCTTCAAGAGGTACCAACTATGGTTGATACAAT GTGTCTGAACTGAAGAGTGAATCTGCTTGTTTAAACAACTTGACCTTGATTGTAGTCCATTTAATAACAG TAATCAGTAATATGGTGTCAGTTTAAGTGGATGGTGTGGTAACTTAAACTCTTGTTCTCATCCCTCAGAC AGGGCATTTTTCCTTGAACACAGAATGGTTTCAGTGAAACCATTCTAGCAGAGAATTAAAGTCAGAACGT TTTAAAGTAATGGTCTTATTGGTGAAGTTTGTGCTTCTTTCCTGAGCTATTTGAAGCTTAAGTATTGCAT AGCTTTGCGCCGTGTGTACTATATTATGAAAGAATATGTAAAGAAAGTGTTCAGTAACACACAGTTTTAA TTTGTGTATAATGGAATGTTATTTACAATGTAACACTGTAAATAAGAAAGCAAAAGTTTATGGGAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4036044 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4036044 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14450 same embryo
	EMAGE:14454 same embryo
	EMAGE:14449 same embryo
	EMAGE:14453 same embryo
	EMAGE:14452 same embryo
	EurExpress:euxassay_004259 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source