EMAGE mouse gene expression spatial database

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EMAGE:14456

Yipf2 Yip1 domain family, member 2 ( MGI:1922016)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004292. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004292_01	euxassay_004292_02	euxassay_004292_03	euxassay_004292_04

euxassay_004292_05	euxassay_004292_06	euxassay_004292_07	euxassay_004292_08	euxassay_004292_09

euxassay_004292_10	euxassay_004292_11	euxassay_004292_12	euxassay_004292_13	euxassay_004292_14

euxassay_004292_15	euxassay_004292_16	euxassay_004292_17	euxassay_004292_18	euxassay_004292_19

euxassay_004292_20	euxassay_004292_21	euxassay_004292_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14456_wholemount_strong.wlz

14456_wholemount_moderate.wlz

14456_wholemount_weak.wlz

14456_wholemount_possible.wlz

14456_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14456_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6172
Entity Detected:	Yipf2, Yip1 domain family, member 2 ( MGI:1922016)
Sequence:	sense strand is shown >T6172 GCCAGCTTAGGTAGGCTCAGGTGAGGAAGGTGGGGTGCATCTGAAGGGACCCAGCTTGGAGCTGAAGTTC CATTCCGTGTATTAGGCTCCATGCAGGAGTCCAGACGGAAGCATCAGTCTTGGGGAGGGCTGTGGGATTG ATGCGGTTCTGTCCACAGAGTGCGATGCGGTGACGCCTCTAAAGAGTCATGGCAGCAGCTGACGAACTGG CCTTCCACGAGTTCGAGGAAGCCACAAATCTTCTGGCAGAGACCCCAGATGCAGCTACTACTAGCCAAAG TGATGAGCTGACCTCCCGAGAGCACGTGGCTGTGGTTGTGGGTTCAGGCATTGGCTATGGAGCTGAAGTA GGGGAGGAAGAGGATGACAAGACATCACTTCTACAGGAAGAAAAACCACAGCCCAGATTCTGGACATTTG ACTACTATCAGAGCTTTTTTGATGTGGATACCTCCCAGGTCTTGGACAGGATCAAAGGCTCCCTGCTGCC CCATCCTGGCCACAACTTTGTGCGGCACCATCTTAGAAACCGTCCCGACCTATATGGCCCCTTCTGGATC TGTGCTACCCTGGCCTTTGTCCTGGCAGTTACTGGCAATCTCACTTTGGTGCTTGCACAGAGACGGGACC CCTCCATTCACTACAGCCCCCAGTTCCACAAGGTGACCATAGCAGGCATCACCATCTACTGCTATGCGTG GCTAGTGCCGCTGGCGCTGTGGGGCTTCCTGCGGTGGCGTCAGGGCACGAGGGAGCGCATGGGGCTGTAC ACCTTCCTGGAGACAGTCTGTGTCTACGGCTACTCACTCTTTGTCTTCATCCCTACTGTGGTCCTGTGGC TTATCCCAGTGCAGTGGATACAGTGGCTCTTTGGAGCCCTGGGCCTGGCCTTGTCAGCAGCCGGGCTGGT ATTCACACTGTGGCCTGTTGTCCGAGAGGATACACGGCTGGTGGCTGCGGCACTATTGTCTACTGTGGTG CTGCTTCACGCCCTCTTGGCGCTAGGCTGTAAGCTGTACTTCTTCCAGCCACTGCCTCTGGACCATGTGG TGCCAGCACCCCAAGCTACACCTCCATCTCCCAATGTCCTGCTGCCCAGTTCAATCCAGCCCATGACAAC CTCCTAGGAAGGCTCATAGGTGAGGGACCAGTTTCTGTGTTGGTTGAGGATGTACCCTCCTCCTGGGACC ATTCCTAGGAGTCTTTCTTTTTCAGGCCAAACTCAAGGGGATACTACTTGTCCTGCCCTCCCTATGAAGA TGGGAGGATTCTTGGACCCTGGAGACTACCCTGCTGTTTTGCAGGCTTTTCTTACAAAGCAAACACTTTT ATTTTCTATGCAAAGGTGATCCAGAGAATTTATATAAAGGTGGGGGAGGGGCAGCCGAGCAAGGAGTATT ACTGATGGACAGGAGAGAGCCTGGTTCCCAGCTGCCTGAGGGCCCTCTGATTCCCTGACAGGTCAGGAGG GTGGCACAGAGCAGCTCCAGCCTTTGCCAAGGGGTTCCTGTGGCAGGCTGTGGCCATTGAGGTGGGGACT CTAGGGTAAGGGAATAGGGGAAACTGCCTGTGCTTATTAATCACCCTTCCTTCTTTGCTTTTCTTCTTGT CTGGGGAGGTCCCTGCGAGGCTCCTTCCCAGGGTTGCTGTCTTGAGAGGATTAAGTAGAACATGAATGTG AACGCAACACACAGGCCTATGTTAGCTATCAGGAGAGGACGCCTTCCTTGTGTGTGTGTGACAGATCTTT GAGGTTTCTTGTCTGCCCCAGTAGCCCAGGGTGACACACTCTGGGCTGCCTGTTCAAGCTTTTGATGGAG GGGGGCCTTCAGGGCTCAGGACAGGACAGTAGCAGAAAGTAGGGACTAGGCCTTTGGACAGCTGGGTGGT TTGTACATTCAAGTTTGAGGTGAACCCTTTGGTGGGAGGGGGCTGTGCCCACTCTGAAGGCTTGACAGGG CCACCCCTCACCTAGAACCTCTGGGCCTAGGAACAGGGGCTGCTGGCTTGGCCCGGCCAGCCGGGTTTCT CAGAGGGTCTGTGGGTTGACACTGGTTCTTTTCGTAAAAAAATAAAATTAAAAAAAGCAGCAAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497525 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497525 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14459 same embryo
	EMAGE:14457 same embryo
	EMAGE:14458 same embryo
	EMAGE:14455 same embryo
	EurExpress:euxassay_004292 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source