EMAGE mouse gene expression spatial database

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EMAGE:14480

Pttg1ip pituitary tumor-transforming 1 interacting protein ( MGI:2652132)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005361. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005361_01	euxassay_005361_02	euxassay_005361_03	euxassay_005361_04

euxassay_005361_05	euxassay_005361_06	euxassay_005361_07	euxassay_005361_08	euxassay_005361_09

euxassay_005361_10	euxassay_005361_11	euxassay_005361_12	euxassay_005361_13	euxassay_005361_14

euxassay_005361_15	euxassay_005361_16	euxassay_005361_17	euxassay_005361_18	euxassay_005361_19

euxassay_005361_20	euxassay_005361_21	euxassay_005361_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14480_wholemount_strong.wlz

14480_wholemount_moderate.wlz

14480_wholemount_weak.wlz

14480_wholemount_possible.wlz

14480_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14480_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5011
Entity Detected:	Pttg1ip, pituitary tumor-transforming 1 interacting protein ( MGI:2652132)
Sequence:	sense strand is shown >T5011 CCACGCGTCCGCCCACGCGTCCGCCTAGCTCGGCGTTGGAGTTGAGTCGCCTGAGGTCCCCGCCGACGAC GCACTCACCATGGCGCCTGCTAACCTTGGGCTGACGCCGCACTGGGTGATGCTCCTCGGTGCCGTGCTGC TGTTGCTTCTGTCCGGAGCCTCCGCGCAGGAACCTCCGAGAGTGGGTTGCTCTGAGTACACAAACAGATC CTGTGAAGAGTGCCTCAGGAATGTCTCCTGTCTGTGGTGCAATGAGAACAAGGCGTGTATGGACTACCCA GTGAGGAAAATCTTGCCCCCTGCTTCTCTCTGTAAATTGAGTTCCGCTCGCTGGGGCGTATGCTGGGTGA ACTTCGAGGCCTTGATCATCACCATGTCGGTCCTGGGGGGCTCTGTGCTCCTGGGCATCACTGTGTGCTG CTGCTACTGCTGCCGCCGGAAGAAGAGCCGGAAGCCAGACAAGAGCGATGAGCGGGCCATGAGAGAGCAG GAGGAGAGGAGAGTGCGGCAGGAGGAAAGGAGGGCGGAAATGAAGTCAAGACATGATGAAATCAGGAAAA AATACGGTCTGTTTAAAGAACAAAACCCGTATGAGAAGTTCTAAGGTGGCTGGCACACACTTGTGGTGGA TCGTGCAGTTCCAGAGTTTCCTGGGAATGCACTCCCCAGCAGAGCCTGCAGAGACCTCACCACCATGGCC ACCCTTGACCTGGGTGATCCCTCCAGCCTCTACTGGCACCAGTGTGGCCTTAAGAGGACAATCCTACTCT TGTTCCCTCTGTGTCTGTATGTAATCACCTGAGACCTTAATCTCGTTGATGTCCTTTTTGGGGGGAGGAA GTATACTTGGTCCCCACACTCTGCAAGCCGATTACGAAGCTGGCAGCCTGAGGTGCTGACTAGCTGCTGA GTGGCCTTGCTGGAAGATTCACATTGCAACAAGCAAGAGTCATGTTTACTTTGTTAAGGCAAGAACTGAC CACCACCCTTGCTTCCTGAAGGCTGTGTTTTGTCTGTAGGCCCACTGCTGTGATGGGCCCTTGTTGTTGT AATTTTGGTGGCCTCTTGGAAGGCACTGTCCCTAAGTCTGGTAAGGATTGCCTTCCTAACCCAGCTCCAT CCAATAGCACAAAAGCTTATAAAGTCACCTGAAGTCTTTAATAATCCCTCAGGAAGCAGTCCTACCAAAA CCTGCCATGAGACGTCCAGGCCTCTTGGATGACATTTTTCATTTTCAGTCTTTACTAGACTGAGAATTTC CTGCTGTGGGAAACCTGAGCTCTGAATTTAGTTTGATCCTCTTGTGAGTGCTGAATGTCCAGCATGCTGG TGCATGGACTCCAGTGTCCTTAGAAGGGCTTTGTGGAGGGTCTTGGACATCTCCCTCCACAGATCAGGCA GCTGTTGAGTTGGTCCTGTATGTATCATGGAGGATGAACAGCATCTCCACTGGCCATCCCATTGTGGGAG CTGAGTGTGTAGGAAGATAGGAGGACCCCACACCATTCTGCCTTCCAGGAAAACTGCCTGAGCTGGCATA GACACCCACCTCACTTCCACCCTGCTTCCAGCCATGCCTCCTCACCTATCGTGCCTGACACATCCGCACG GGCCCTGGGAAGGTTGTCAGGAGACACTCGGGTCCCTGAGATTCCGTCCACGCTGTACATTTCTTTGAAA TCCAGATGTGTCCTAGTAAGCAGATCACAACCCTGATGGCAGGGTAAGGAGTAAAGATATTCCCTCTGGG AAAGCAGCCACCAGCAGATGAGGTGGTCTTACTGTAGGCTCCCTCATGCCTAAGACCTCACCTAGCATCT CGGTAACCTTTTCACCTGTCAGTCTGTCCAGGGATTAATATTTTCCTCTCTTCAGATTTCCACAAAGTAA GGGAATAACCTTTACCACAATCTGGAGTCTTACCATTTTTCTGCTTTGCTAGGCATTTGATGGAACTTAA AGCCTTGTCAAACATTTCTTAAGTATTATAGCAGGCATTGAGAGTTATCTTTCTCCTGTCTATTGAAAGG GAACCCTCCTTACAGGCCCTGATGAACTTCGTATTAATATGTCGCCTGTATAAATTGTACCTGTTAGGTT GCTGAGCTGATGTTAGAGTATTTAATTCTCCAGCCTAAGGAGTGCAGCAGGTGCTCGTGGGAGTGGTCTG TGCCTACCTTTGAGCAGCATGAAGCCACAGAACCAAGCCTGACACCATCACACCCCGTGCCTTAGTTTTT AACCACTGTCCAATAAAATGGTTGAATATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5250502 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5250502 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14484 same embryo
	EMAGE:14483 same embryo
	EMAGE:14482 same embryo
	EMAGE:14481 same embryo
	EMAGE:14478 same embryo
	EMAGE:14479 same embryo
	EurExpress:euxassay_005361 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source