EMAGE mouse gene expression spatial database

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EMAGE:14504

Limk2 LIM motif-containing protein kinase 2 ( MGI:1197517)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005391. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005391_01	euxassay_005391_02	euxassay_005391_03	euxassay_005391_04

euxassay_005391_05	euxassay_005391_06	euxassay_005391_07	euxassay_005391_08	euxassay_005391_09

euxassay_005391_10	euxassay_005391_11	euxassay_005391_12	euxassay_005391_13	euxassay_005391_14

euxassay_005391_15	euxassay_005391_16	euxassay_005391_17	euxassay_005391_18	euxassay_005391_19

euxassay_005391_20	euxassay_005391_21	euxassay_005391_22	euxassay_005391_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14504_wholemount_strong.wlz

14504_wholemount_moderate.wlz

14504_wholemount_weak.wlz

14504_wholemount_possible.wlz

14504_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14504_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6160
Entity Detected:	Limk2, LIM motif-containing protein kinase 2 ( MGI:1197517)
Sequence:	sense strand is shown >T6160 CCCACGCGTCCGCCCACGCGTCCGAGGGGCAGAGACAGCCTCTTCTCTAGACCGCAGGCGGGCGCGGACC TGAGGCGCTGCTGCCGGGTGGTCCTTCCTGTGTTGTCCGCGCCCTGGACCATGGCGGCGCTGGCGGGTGA TGAAGCCTGGAGGTGTCGGGGCTGTGGCACCTATGTTCCCCTGAGCCAGAGGCTCTATAGGACTGCCAAT GAAGCCTGGCATGGCTCCTGCTTCCGGTGTTCCGAATGCCAGGAATCCCTCACCAACTGGTACTATGAGA AGGATGGGAAGCTGTACTGCCACAAGGACTACTGGGCCAAGTTTGGAGAATTCTGCCATGGGTGCTCCCT GCTGATGACGGGGCCAGCCATGGTGGCTGGGGAGTTTAAGTACCACCCAGAGTGCTTTGCCTGCATGAGT TGCAAGGTGATCATTGAGGATGGAGATGCATATGCCCTGGTGCAGCACGCTACACTCTACTGTGGGAAGT GCCACAACGAGGTGGTGCTGGCACCCATGTTTGAAAGACTGTCTACAGAGTCTGTCCAGGATCAGCTGCC CTACTCTGTCACGCTCATCTCCATGCCTGCTACCACCGAGTGTAGACGCGGCTTCTCGGTGACTGTGGAA AGTGCCTCTTCTAACTATGCCACCACCGTTCAAGTGAAAGAGGTCAACCGGATGCACATCAGTCCCAACA ACCGAAATGCCATCCACCCTGGGGACCGCATCTTGGAGATCAATGGGACCCCTGTCCGTACTCTGCGAGT AGAGGAGGTGGAGGATGCAATAAAGCAGACAAGCCAGACACTTCAGCTGCTGATTGAACATGACCCTGTG CCCCAGCGCCTGGACCAGCTACGGCTAGATGCCCGACTTCCTCCCCACATGCAGAGCACCGGACACACGC TCATGCTCAGCACCTTGGACACCAAGGAGAATCAGGAGGGGACACTGAGGAGACGTTCTCTGAGGCGCAG TAACAGCATCTCCAAGTCTCCGGGCCCCAGCTCCCCCAAGGAGCCCCTGCTCCTCAGCCGGGACATAAGC CGCTCAGAATCCCTCCGCTGCTCTAGCAGCTACTCCCAGCAGATCTTCCGGCCCTGCGACCTGATCCACG GGGAGGTCCTGGGGAAGGGCTTCTTTGGGCAGGCCATCAAGGTGACTCACAAAGCCACAGGCAAAGTGAT GGTCATGAAGGAGTTGATCCGCTGTGATGAGGAAACCCAGAAGACTTTCCTGACTGAGGTGAAAGTGATG CGGAGCCTGGACCACCCTAATGTGCTCAAGTTCATCGGAGTTCTGTACAAGGACAAGAAGCTGAATCTGC TGACCGAGTACATCGAGGGAGGCACACTCAAAGACTTCCTGCGCAGTGTGGACCCGTTCCCCTGGCAACA GAAGGTCAGGTTTGCCAAAGGCATCTCCTCTGGAATGGCCTATTTACACTCCATGTGCATCATCCACCGG GACCTGAACTCACACAACTGTCTCATCAAATTGGACAAGACAGTGGTAGTAGCCGACTTTGGGCTGTCAC GGCTTATAGTAGAAGAGAGGAAAAGGCCTCCAGTAGAGAAGGCCACCACCAAGAAACGCACCTTACGCAA GAGTGACCGCAAAAAGCGCTACACTGTGGTGGGAAACCCCTACTGGATGGCCCCCGAGATGCTGAATGGC AAGAGCTACGATGAGACGGTGGATGTCTTCTCTTTTGGGATCGTTCTCTGTGAGATCATTGGGCAGGTAT ATGCGGATCCTGATTGCCTGCCCCGCACACTGGACTTTGGCCTCAATGTTAAGCTTTTCTGGGAGAAGTT TGTCCCAACAGACTGTCCCCCAGCCTTCTTCCCCCTGGCTGCTATCTGCTGCAAACTAGAGCCTGAAAGC AGACCGGCATTCTCAAAGCTGGAGGACTCGTTCGAGGCGCTCTCCCTGTTCTTGGGGGAGCTGGCCATCC CACTGCCAGCAGAGCTGGAAGACCTGGACCACACTGTGAGCATGGAGTATGGCCTAACCCGGGACTCGCC ACCCTAGCCCTGGTCCCTCCCCCTGCAGGGGGACATTCCACAGCCAGCATTGCCCCCTCTGCACGCTGTC CTTGCTGTGAGCAGGACTGCCTAGGTTTCCTGTGGATTGAAGGCTTGCTTAGAGGCACAACAAGCCATCC CTACTACCTCCCCAGGAGGCACTTGGGTGCAGGGAAAAAAATTCTCCGCAAGTTTTGGGGGCCTAGTTTC TATCTGTAAATCCAACACTCACCTGAAAGCTGTGAAGAGGATAAAAGAGCCTGGATTATAGACCAAGAGT CTGTTACTCGGGACTACTTGGGACCTCTGTGGATTGAGATTCTGGGAGGCTGTAGCTTTACACTAATCAA CATGATCTGGGCCTGCTGGGCAAGATGACGGAGTGAACTGGCCTGAGGGCTCTGGAGTCTGGCTCAGCAA GGTGCAGGACTTGAAGCGGTAGTATGAGTTACAGAAACGGGGAACCTTCAAAGCATGTGAAATGACAGTA GCGATTTCTCCTCCATCCCAGGCCATGCCCTTTGGAGCAGGTTTTAAAGAGTCTTAGTATGTGGTAAAAC CTACAGAATCCAACAAAGGACTGGTCTCTGCTCACAGCCCAGGGACCACATCAGTGCATGGGGAGTTTCC ACTTCAGCATCCTGTCTCAACAAGCAGTCACTCACAAAAACACCAAAGAAACTGGGGCTGCAAACAGGCC CTGCCCTCTAAAAGCCCTGTATCCAGGCTGGTGGGCTCAGTCAAAAGCTCAGCATGTTGCCTAACAGTAC ATGGGTTCTGGATGCCAGCCTGGTTTGCCTCAGACTTTGGGTCTAAGCAGAGAGAAGGGGGTCCCATCAA TTTCTTTGCACCCTTGTGGCAGCTACTGCACATCCTCTAGAATGTCTGGGCCAGGCAGCAATGTGGGACA GGCATTGGTGGCTGCTGAGTCCCCAAACTGAGATAGGAAAAGAACACCCTGAGCTGGGCCTTGTAGATTC TACCTCTTTTTGTTGCTGGTCCAGCTTGCTTCCTGAGCCACACTCGAGGGCCATGGGCATGGGCAACTAT GGGGGCACTTTCTACCCCTGGCTGCCAGGTGTCCAGGGACTCTGTAGCTCACCAAGGTTTGTCATCTAAG CGGGAGCTCACGCCATGTTTAACAAATTGGGAATGGGTTTGGTTATAAATACATGTGATGGGTTGTACTG GTGGGGGGATTCAGGAAGAGTGGTTGCTGTTATTAGCTTTTATCTCTGGGGTGATATGTGAGATATTTTG TACATAGACGAAATGAATTATGGGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3496836 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496836 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14508 same embryo
	EMAGE:14505 same embryo
	EMAGE:14506 same embryo
	EMAGE:14507 same embryo
	EMAGE:14503 same embryo
	EurExpress:euxassay_005391 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source