EMAGE mouse gene expression spatial database

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EMAGE:14516

Prkrip1 Prkr interacting protein 1 (IL11 inducible) ( MGI:1914051)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005378. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005378_01	euxassay_005378_02	euxassay_005378_03	euxassay_005378_04

euxassay_005378_05	euxassay_005378_06	euxassay_005378_07	euxassay_005378_08	euxassay_005378_09

euxassay_005378_10	euxassay_005378_11	euxassay_005378_12	euxassay_005378_13	euxassay_005378_14

euxassay_005378_15	euxassay_005378_16	euxassay_005378_17	euxassay_005378_18	euxassay_005378_19

euxassay_005378_20	euxassay_005378_21	euxassay_005378_22	euxassay_005378_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14516_wholemount_strong.wlz

14516_wholemount_moderate.wlz

14516_wholemount_weak.wlz

14516_wholemount_possible.wlz

14516_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14516_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8317
Entity Detected:	Prkrip1, Prkr interacting protein 1 (IL11 inducible) ( MGI:1914051)
Sequence:	sense strand is shown >T8317 CTGATGTTGGAGAGCGGCTATGGCGAGTCCTGCTGCCGCTTCTGTGAGACCGCCCAGGCCCAAGAAAGAG CCGCAGACGCTGGTCATCCCCAAGAATGCGGCCGAGGAGCAGAAGCTCAAGCTGGAGCGGCTCATGAAGA ACCCGGACAAAGCAGTTCCAATTCCAGAGAAAATGAATGAATGGGCTCCTCGAGCGCCTCCAGAATTTGT CCGAGATGTCATGGGTTCCAGTGCTGGGGCTGGCAGTGGAGAGTTCCACGTGTATAGGCACCTACGGCGG AGAGAGTACCAGCGGCAGGACTACATGGATGCCATGGCTGAGAAGCAAAAACTGGATGCAGAGTTTCAGA AGAGACTAGAAAAGAATAAAATTGCTGCAGAGGAGCAGACTGCAAAGCGCCGGAAAAAGCGCCAGAAGTT AAAAGAGAAGAAGTTACTGGCAAAGAAGATGAAACTTGAACAGAAGAAACAGAAGGAAGAACCCAGTCAG TGCCAGGAACAGCATGCCAGCAGCTCTGACGAGGCATCTGAAACAGAGGAGGAGGAAGAGGAGCCCAGCG TCGTCATCATGGGGCGATGAGGAGTTTCAGCAGTCGTGAGCCCAGCTCATTCTGTGACCAGCCTCAGAAG AGAAATGGCTGTGTGTGACCAGCTGCTAGACTGGCTGTGTTGGGAGAAGGCAGGCACCTCCTGCAGCCCT GCCTGCCTGGGGAGCAGAGGGAGCAGACCTTAGACATTTGCAGATTGCATTCTGTATCCTGCCCTGCTCT TGCTGAAGGGGAGTGAGTGCTTCACCTTCCCCGGGTGCCGTTCCCTTCTGCTTGAACAGCAGTGAGCTCT GCGCTGGTTTCTGTGAAGGGAATATTTATTTAGTAAAGAACAGAAAACCTGTAAAAAAAAAAAAAAAAAA GAAA
Notes:	The probe template was PCR amplified from IMAGE:1494676 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1494676 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14515 same embryo
	EMAGE:14513 same embryo
	EMAGE:14514 same embryo
	EMAGE:14511 same embryo
	EMAGE:14512 same embryo
	EurExpress:euxassay_005378 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source