EMAGE mouse gene expression spatial database

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EMAGE:14518

Slpi secretory leukocyte peptidase inhibitor ( MGI:109297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005396. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005396_01	euxassay_005396_02	euxassay_005396_03	euxassay_005396_04

euxassay_005396_05	euxassay_005396_06	euxassay_005396_07	euxassay_005396_08	euxassay_005396_09

euxassay_005396_10	euxassay_005396_11	euxassay_005396_12	euxassay_005396_13	euxassay_005396_14

euxassay_005396_15	euxassay_005396_16	euxassay_005396_17	euxassay_005396_18	euxassay_005396_19

euxassay_005396_20	euxassay_005396_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14518_wholemount_strong.wlz

14518_wholemount_moderate.wlz

14518_wholemount_weak.wlz

14518_wholemount_possible.wlz

14518_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14518_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10

liver right lobe

strong

single cell

strong expression: see section 11 12 13 14 15 16 17 18 19 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8327
Entity Detected:	Slpi, secretory leukocyte peptidase inhibitor ( MGI:109297)
Sequence:	sense strand is shown >T8327 GCTTCTGTCATTTTCAGCTCTCAGGTGGTTACTCTGATGGCCTCATGGTCCTGCCTGAAACAGAAAGTCT GCCACCTACTTCTGTAGCAGCAAGACTCCTGTTCTGTGGCTAAGCTTCCTGCCTGTGCAAGAGCCACAGG GAGGGGCCAAATGCATGCCACTGGGGCCACGCCTCCTGGTAAAGACATAAATAGTGATCCTCGGGACTGG TCATCAGAGCTCCCCTGCCTTCACCATGAAGTCCTGCGGCCTTTTACCTTTCACGGTGCTCCTTGCTCTG GGGATCCTGGCACCCTGGACTGTGGAAGGAGGCAAAAATGATGCTATCAAAATCGGAGCCTGCCCTGCTA AAAAGCCTGCCCAGTGCCTTAAGCTTGAGAAGCCACAATGCCGTACTGACTGGGAGTGCCCGGGAAAGCA GAGGTGCTGCCAAGATGCTTGCGGTTCCAAGTGCGTGAATCCTGTTCCCATTCGCAAACCAGTGTGGAGG AAGCCTGGGAGGTGCGTCAAAACTCAGGCAAGATGTATGATGCTTAACCCTCCCAATGTCTGCCAGAGGG ACGGGCAGTGTGACGGCAAATACAAGTGCTGTGAGGGTATATGTGGGAAAGTCTGCCTGCCCCCGATGTG AGCCTGATCCCTGACATTGGCGCCGGCTCTGGACTCGTGCTCGGTGTGCTCTGGAAACTACTTCCCTGCT CCCAGGCGTCCCTGCTCCGGGTTCCATGGCTCCCGGCTCCCTGTATCCCAGGCTTGGATCCTGTGGACCA GGGTTACTGTTTTACCACTAACATCTCCTTTTGGCTCAGCATTCACCGATCTTTAGGGAAATGCTGTTGG AGAGCAAATAAATAAACGCATTCATTTCTCTATGCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1513866 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1513866 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14519 same embryo
	EMAGE:14521 same embryo
	EMAGE:14520 same embryo
	EMAGE:14522 same embryo
	EMAGE:14517 same embryo
	EurExpress:euxassay_005396 same experiment
	MGI:4828311 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source