EMAGE mouse gene expression spatial database

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EMAGE:14522

Tnip2 TNFAIP3 interacting protein 2 ( MGI:2386643)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005399. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005399_01	euxassay_005399_02	euxassay_005399_03	euxassay_005399_04

euxassay_005399_05	euxassay_005399_06	euxassay_005399_07	euxassay_005399_08	euxassay_005399_09

euxassay_005399_10	euxassay_005399_11	euxassay_005399_12	euxassay_005399_13	euxassay_005399_14

euxassay_005399_15	euxassay_005399_16	euxassay_005399_17	euxassay_005399_18	euxassay_005399_19

euxassay_005399_20	euxassay_005399_21	euxassay_005399_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14522_wholemount_strong.wlz

14522_wholemount_moderate.wlz

14522_wholemount_weak.wlz

14522_wholemount_possible.wlz

14522_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14522_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8730
Entity Detected:	Tnip2, TNFAIP3 interacting protein 2 ( MGI:2386643)
Sequence:	sense strand is shown >T8730 CTGGTTTTCGCTCCCCTGTGTGGAGAAGTTGGAGACGCCCAAGTCCCCACGGAAGGCCTACAGCCATGTC GTCTGGGGACCCAAGGTCTGGTAGACAGGACGGGGCCCCGCGTGCGGCCGCAGCGCTCTGTGGCCTGTAC CACGAGGCCGGCCAGCAACTACAGCGCCTGAAGGATCAGCTGGCCGCGCGTGACGCCCTCATCGCGAGCC TCCGCACCCGCCTCGCGGCTCTGGAAGGGCACACGGCGCCGTCACTCGTGGACGCACTTCTGGATCAGGT GGAGCGCTTCCGTGAGCAGCTGCGACGACAGGAGGAAGGCGCTTCGGAGACCCAGCTGCGGCAGGAAGTT GAAAGACTTACGGAGCGTCTAGAGGAAAAAGAGAGGGAGATGCAACAGCTGATGAGCCAGCCTCAGCATG AGCAAGAGAAGGAGGTAGTCTTGCTTCGGCGAAGTGTGGCAGAGAAGGAGAAAGCCAGGGCCGCCAGTGA TGTTCTGTGCCGCTCCTTGGCTGATGAGACCCACCAACTGCGCAGGACATTGGCAGCCACTGCCCACATG TGCCAACATCTGGCCAAATGTCTGGATGAACGACAGTGTGCACAGGGAGACGCTGGGGAGAAAAGCCCTG CTGAGGAGGTAAGATGGATCTGTGGCCAGGCCTACACCAAGGACCTGAAAAAGCCTCCTGGATGCTGGCT AGAGCAAACAAGCAGCGATGCTTCTGGCCAGAGTGTTATTAAGAAGTTACAGGAAGAAAATCGACTGTTA AAACAGAAGGTGACTCATGTAGAAGACCTCAATGCTAAGTGGCAGCGTTATGATGCAAGTAGGGACGAAT ATGTGAAGGGGTTGCATGCCCAGCTAAAGAGGCGGCAGGTCCCTCTGGAGCCTGAGCTGATGAAGAAGGA GATTTCCCGACTTAACAGACAGTTGGAGGAGAAAATAAGTGACTGTGCGGAAGCAAACCAGGAGCTGACA GCCATGAGGATGTCCCGGGACACTGCGCTGGAGCGAGTGCAGATGCTAGAACAGCAGATTCTTGCTTACA AGGATGACTTCAAATCAGAAAGGGCAGATCGGGAACGAGCGCACAGTAGGATTCAAGAGCTGGAGGAAAA GATCATGTCCTTGATGTACCAAGTGTCCCAGAGACAGGACTCCCGGGAGCCAGGACCCTGTCGGATTCAT ACGGGGAACAAAACTGCCAAGTACTTAGAGATGGATGCACTGGAGCATGTGACCCCTGGCGGCTGGAGGC CTGAGTCTAGGTCCCAACAGATGGAACCTTCTGCAGAGGGTGGGCATGTGTGCACAGCCCAGAGAGGTCA GGGTGACCTTCAGTGCCCTCATTGCCTGCGGTGCTTCAGTGATGAGCAAGGCGAGGCATTCCTCAGGCAC CTGTCTGAGTGCTGCCAATGAGCCAGACATTGCCCGTGTGACCCATGACCACCATAGCTGCTCTAAGGGA CTGGGAGGGGTCCTCAGACTCAGTTTTCAACTCAGTGTGTTGCATTCTCCTGGGATCTAGGGCCCAAATG GGCAGGGTCACTGGAAGGTCATCTTGTTTTCATTTGACCATGGTGAGACTTGGTCAGAGGGAACTATTGA CAGAGCAGGAGGAAGAGGTGGGGTCAGGGACATCAAGTGGACATCAGTTTTGTCTCACGTAGAGTTTGGA GTGAGCTGTCAATTCAAAGCTGCAAGCTATCAGTTGTGGGAATATTCTGAAGCCTGCTTGCACCTAGAGT TATGCCACTTGCTGGAAGGGGAAGTTGCTGTGGGAGCAGTGTGTCCTCTTTCCAGGGTGGTAGCTCCATC CTGTTGAGTAGTGAGATACACTCCCTGACTGGTCTGTGCTGCATTACAGTTACATGATACACTAGAACCT TCCCAAACTCAGCAGAGCCACACAGCTGCATCCAGTACCATCACCCTGCAAAACACTTGTATTTCCAAAA GGGAAAGCACCTTTATTTCCTAATCATTTATTTTTATAATAAATGGCTTTACTACTGTGTTAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6404610 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6404610 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14519 same embryo
	EMAGE:14521 same embryo
	EMAGE:14520 same embryo
	EMAGE:14518 same embryo
	EMAGE:14517 same embryo
	EurExpress:euxassay_005399 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source