EMAGE mouse gene expression spatial database

EMAGE:14557

Pln phospholamban ( MGI:97622)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005434. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005434_01	euxassay_005434_02	euxassay_005434_03	euxassay_005434_04

euxassay_005434_05	euxassay_005434_06	euxassay_005434_07	euxassay_005434_08	euxassay_005434_09

euxassay_005434_10	euxassay_005434_11	euxassay_005434_12	euxassay_005434_13	euxassay_005434_14

euxassay_005434_15	euxassay_005434_16	euxassay_005434_17	euxassay_005434_18	euxassay_005434_19

euxassay_005434_20	euxassay_005434_21	euxassay_005434_22	euxassay_005434_23	euxassay_005434_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9824
Entity Detected:	Pln, phospholamban ( MGI:97622)
Sequence:	sense strand is shown >T9824 GCAGCTGCTCCCATAAACCTGGGAACAGAAAACTGCCCAGCTAAGCTCCCATAAGACTTCATACAGCTTC ATGCTCTGCACTGTGACGATCACCGAAGCCAAGGTCTCCTAAAAGAAGACAGCTCACATTTGGCTGCCTG TTGTCAACTTTTTATCTTTCTCTTGACCACTTAGAAAAACTTGTCTCCCTACTTTTGCCTTCCTGGCATA ATGGAAAAAGTGCAATACCTCACTCGCTCGGCTATCAGGAGAGCCTCCACTATTGAAATGCCTCAGCAAG CACGTCAGAATCTCCAGAACCTATTTATCAATTTCTGCCTCATCTTGATATGTCTGCTGCTGATCTGCAT CATTGTGATGCTTCTGTGAAGAGCTGCCGCCACTCCAGACCTGCAACATGCCAACTCAGCTTAAAGCCGA GCACTCCGTCATGGGGGAAGCAACGCACCGCTGCCTTCCGGCTGAGAACAGCTTTGTGAGGGTCACGATT AAGAGTGAAACTAATTGTTAGCCAGTGTATTCATCTGCTGGATCTTGTAAACAAACTTTACAGTGGCTAA GTGTGCATAATGTAGCTGCCAATTTCCTCAACATGGCTCATAAATTTCTTTCCTAAATAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6772294 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6772294 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14562 same embryo
	EMAGE:14558 same embryo
	EMAGE:14561 same embryo
	EMAGE:14560 same embryo
	EMAGE:14559 same embryo
	EurExpress:euxassay_005434 same experiment
	MGI:4827277 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source