EMAGE mouse gene expression spatial database

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EMAGE:14562

Tsc22d1 TSC22 domain family, member 1 ( MGI:109127)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005439_01	euxassay_005439_02	euxassay_005439_03	euxassay_005439_04

euxassay_005439_05	euxassay_005439_06	euxassay_005439_07	euxassay_005439_08	euxassay_005439_09

euxassay_005439_10	euxassay_005439_11	euxassay_005439_12	euxassay_005439_13	euxassay_005439_14

euxassay_005439_15	euxassay_005439_16	euxassay_005439_17	euxassay_005439_18	euxassay_005439_19

euxassay_005439_20	euxassay_005439_21	euxassay_005439_22	euxassay_005439_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14562_wholemount_strong.wlz

14562_wholemount_moderate.wlz

14562_wholemount_weak.wlz

14562_wholemount_possible.wlz

14562_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14562_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8806
Entity Detected:	Tsc22d1, TSC22 domain family, member 1 ( MGI:109127)
Sequence:	sense strand is shown >T8806 CTGGAGGGGGAAGTGAATGGCGAAGGACTGAAGGGGTCCCCCTTCGGTTCCCCAGCCACCTGGTTCACCC GGTTCATCCTCCCTTCCCGAAGTTAGCCCTCGAAGGCACGAGCAGCCGGCGCCTTCGGCAGAGGAGGAGG AGGAGGAGGGATCGCGTCGGGCGGAGCTTCAGGTCCTGTTCGTCTCTCCGGCTTCTCCATACAAAGATTA CGGTGCAGAAGGAAATTGCACTTGCCTCCTCCGCCCCCCGGTACCCAGCACAATGCACCAGCCGCCGGAG TCCACCGCCGCGGCGGCCGCTGCTGCAGACATTAGTGCTAGGAAGATGGCGCACCCGGCAATGTTCCCTC GAAGGGGCAGCGGTGGTGGCAGCGCCTCTGCTCTCAATGCAGCAGGTACGGGCGTCAGTGGTGCTGCTCC ATCTTCTGAAGATTTTCCTCCTCCTTCGCTGCTCCAGCCGCCGCCTCCTGCTGCATCTTCCACGCAGGGA CCCCAGCCTCCGCCTCCACAAAGCCTGAACCTCCTCTCGCAGGCTCAGCTGCAGGGACAGCCTCTTGCGC CAGGCGGAACTCAGATGAAAAAGAAAAGTGGCTTCCAGATAACTAGCGTTACTCCGGCTCAGATCTCTGC CAGCATCAGCTCCAACAACAGCATCGCGGAGGACACCGAGAGCTACGACGATCTGGATGAGTCGCACACG GAAGATCTCTCCTCTTCCGAGATCCTCGACGTCTCCCTTTCCAGGGCCACTGACTTAGGTGAGCCTGAAC GAAGCTCCTCAGAGGAGACTCTGAATAACTTCCAGGAAGCTGAGACACCTGGGGCAGTCTCTCCTAACCA GCCCCACCTTCCTCAGCCTCATTTGCCTCACCTTCCACAACAGAACGTTGTGATCAATGGGAATGCCCAT CCCCACCACCTCCATCATCACCATCACCCTCATCATGGGCACCACCTTCACCATGGGCACCATCATTCAT CACATGCTGCTGTGGCCGGTCCATCCATTCCCGGAGGGCCACCCTCGAGCCCAGTGTCCAGGAAACTCTC TACAACTGGAAGTTCTGATGGTGGTGTGCCAGTCGCACCTCCTCCTGCAGTACCATCGAGTGGCTTACCA GCATCAGTGATGACGAACATCCGTACTCCAAGTACTACAGGAAGCCTAGGTATAAATTCGGTTACTGGCA CGAGCGCTACGAATAATGTTAACATTGCTGCTGTGGGTAGTTTCAGTCCCAGTGTGACGAACAGCGTGCA TGGTAATGCTAATATAAATACAAGCAATATCCCTAATGCTGCTAGCATAAGTGGTGGGCCTGGAGTTACC AGTGTTGTTAATTCGAGTATCTTGAGTGGCATGGGCAATGGTACCGTTTCTTCCTCTCCTGTTGCTAACA GTGTCCTTAATGCAGCTGCAGGTATCACTGTGGGAGTGGTTTCCAGTCAGCAGCAGCAGCAGCAGCAGCA GCAACCAACAGTTAACACATCGAGGTTCAGGGTTGTGAAGTTAGACTCTACTTCTGAACCCTTTAAAAAA GGTCGATGGACTTGCACAGAATTCTATGAGAAGGAAAACGCTGTGCCAGCTACAGAAGGCGTGGCCGTCA ATAAAGTGGTGGAGACGGTGAAGCAAACCCCCACGGAAGCATCGTCCTCGGAGAGGGAGAGCACTAGTGG GAGTTCTGTGAGCAGTAGTGTGAGCACACTGAGTCACTACACGGAGAGTGTGGGAAGTGGAGAGATGATG GGAGCCCCGGCTGTGGTGGCGCCGCAGCAGCCGCCGCTACCACCAGCGCCTCCAGGTCTTCAAGGTGTGG CTCTCCAACAGCTAGAGTTCAGTAGCCCTGCTCCACAGAGTATTGCGGCGGTTAGTATGCCACAGAGTAT TTCTCAGTCACAGATGTCACAAGTACAGTTACAGCCTCAAGAGTTGAGCTTTCAGCAGAAGCAGACTCTT CAGCCTGTCCCTCTGCAAGCCACCATGAGTGCCGCAACTGGTATCCAGCCTTCCCCTGTCAGCGTGGTCG GCATCACGGCGGCTGTAGGTCAGCAGCCTTCTGTTTCCAGCCTGGCTCAACCGCAGCTGCCATATTCTCA GACAGCTCCTCCCGTGCAAACTCCTCTTCCAGGGGCACCACCCCAGCAGTTACAATATGGGCAGCAGCAA CCAATGGTTCCTGCGCAGATAGCCCCAGGCCATGGCCAGCCAGTGACTCAAAATCCAACTTCAGAGTATG TGCAGCAGCAGCAGCAGCCAATATTTCAAGCAGCATTGTCCTCTGGGCAGTCCAGTTCCACGGGCACGGG GGCAGGAATATCAGTGATTCCTGTGGCTCAGGCACAGGGGATCCAGCTGCCAGGGCAGCCCACAGCAGTA CAAACACAACCTGCAGGGGCGGCTGGGCAGCCCATTGGCCAGGCTCAAACAGCAGTGTCCACTGTACCAA CTGGCGGTCAGATTGCAAGTATCGGTCAACAGGCAAACATACCCACTGCAGTACAGCAGCCCTCTACCCA AGTTACACCTTCAGTTATTCAGCAAGGTGCTCCTCCATCTTCACAAGTAGTCCTACCTGCTCCAACCGGG ATCATTCATCAGGGAGTTCAAACCCGTGCTTCAAGCCTTCCACAACAATTGGTCATTGCACCCCAGAGTA CCTTGGTAACTGTGCCTCCCCAGCCACAGGGAGTAGAAACCGTGGCCCAAGGGGTTGTTTCCCAGCAGTT GCCCACAGGCAGTCCTCTGCCCTCTGCTAGCACTATTTCTGTTACAAATCAGGTTAGTTCAGCCGCTCCT TCTGGAATGCCTTCTGTCCCAACAAACTTAGTTCCACCACAGAATATAGCACAACCCCCAGCCACCCAAA ATGGCAGTTTGGTTCAAAGTGTTAGTCAATCTCCCTTGATAGCCACTAACATAAATTTGCCTTTGGCACA ACAGATACCACTAAGTTCTACTCAGTTCTCTACACAATCATTAGCTCAGGCCATTGGAAGCCAAATGGAA GATGCCAGGCGCCCAGCGGAGCCCTCCTTAGGCGGCTTACCTCAGACTATGAGTGGTGACAGTGGGGGAA TGTCAGCAGTTTCAGATGGGAGTAGCAGCAGCCTAGCAGCCCCTGCTTCTCTCTTCCCGTTGAAGGTGCT ACCGCTGACAACACCCCTGGTGGATGGCGAGGACGAGAGCTCTGGTGCAAGTGTGGTAGCTATCGACAAC AAAATAGAGCAAGCTATGGATCTGGTGAAAAGCCATTTGATGTATGCGGTGAGGGAGGAAGTGGAAGTTC TGAAGGAGCAGATCAAAGAACTAATAGAGAAAAACTCCCAGCTGGAGCAGGAGAACAATCTGCTGAAGAC GCTGGCCAGTCCGGAGCAGCTCGCCCAGTTTCAGGCCCAGCTGCAGACTGGCTCCCCTCCGGCCACCACG CAGCCACAGGGGACCACACAGCCCCCTGCACAGCCAGCATCCCAGGGCTCAGGATCAACCGCATAGCCTC CTAGGCCCCAACAGAACTGGCTGCTGCTGCTGCTGTCTGAACTGAACAGACCGAAGAGATGTGCTAGAGA GAAGCCGCCTCCACAGTCACCCATTTCATTGCTGTCTACGAAAGAGACGTGAGACTCACACGCTGTTCTC GCTTTCTCCCCAGTATTAAGCACTCATAAGCTTTTGGCTTGAAGAAATGTACTAGTTGAGTGAATTAAAG GTTAATCAGAGAGTGAGCAGGGATGTGCCCTGTGCAACGTGGCAGATGTCTGAGGAATGGTTTAATTGAC CCCGAGGAGCTCTGTGCCTTTTCAACCCTCCCCAGCCGCCCACCCTGCTTCTGAGAGCTCGGGCGGCTCG CCTTCGTGGGGCTCGCCTGCGTGGGGTTCGGAAAGTGGGCTGCTCCTGGATTCTGCGCTCTCTTCTCCTT CCCTTCAAAGAACTCGGAGAGGCCAGAAACAAGACTGCAATGGGGGGCGGGGGGAGGGATGATGCAGTCC TTATACAAAACCGACAACTGTCACCAAAGCTTATAAAACACGATAGTACTGTCCCTCTTTTCTGAACCAT CAGAAGACACAAAACTGTTAGTGACATAACGGTGACAGGTAGCTGGGACCTAGGCTATCTTATTATGAAG GTTGTTTTGCTTGTTGTATATTTGTGTATGTAGTGTAACGAATTTGTACCATAGAGGACTGTCCGTAACT ACTGTTTAGCTTCTACACATTGAAATGTAGATGTTTCATTGGCTGTCTGAAAAGGTGTGGCTTGTCCTTC CTAGAGAGATCTACTTAAAAACTGCTTTGTGACAAAAACCACACCTGAAGAAATTTTAAGAATTTGGCCC AGTTAGTCACTCTGTGTAATCCCGGAATCTAGCTGCTGAAGTCTTGCGAAGTAAACTCCCCGTGACCGAT GTCAGTTAAGCTGGTGATACCTGGAGAAGTGGTCAGTTGCTAAGGAAGTGGATTTCCCAGTAGGGGTTTC TGCACCTCACCTGTATAGTCGTTCTGCGCATGTCCCCCACACAGTCCCCACTGTATTTACCTGTTCTACT TGTCACCTTTCAATAAAGCATATCAAATGTTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409347 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409347 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14557 same embryo
	EMAGE:14558 same embryo
	EMAGE:14561 same embryo
	EMAGE:14560 same embryo
	EMAGE:14559 same embryo
	EurExpress:euxassay_005439 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source