EMAGE mouse gene expression spatial database

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EMAGE:14563

Ints6 integrator complex subunit 6 ( MGI:1202397)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005414_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14563_wholemount_weak.wlz

14563_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14563_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8804
Entity Detected:	Ints6, integrator complex subunit 6 ( MGI:1202397)
Sequence:	sense strand is shown >T8804 AGTGGTGACACCGCTGGGGTGCGGAGGGAGTCTCTCAAACTTCTCTCAACAGCCCCAGGGCCAATCTCCC CTCAGCCCACTCCCCTCTTCGCCAGATCCCTGGTCCCCACTCCCTGCACTGGGGGTGGGGTGGGGAACTG GGCCCGTCTCCCGCGGGGACACACACAGGGTCCGGGAGTGGGGACGGGACCCCCAGGCCGGGGGGGACGG ACGGACAGACGGCCGCGCGGCCGCCCCAGCGGGCAGGCAGGCCGGAGGCGGGGGTACGGCCGGGAGGACC TGGGCTGGGGAGTTTCCTCTCAACTATCGGGGGAGAAACTCCCCATAGCTGGTGTTTTCCTCCCGGGGGC CGCGTTCCCCGCCCCGCGTATCGGCGGTTGCCGCCGCCGCCGCCGGTGCTTCCACCTCTGCCATCTCTTC ACCACCTCGGGCCCCGGTGTCCCCGGCCAGCACTATGCCCATCTTACTGTTCCTGATAGACACGTCTGCC TCTATGAACCAGCGCAGCCATCTGGGCACCACCTACCTGGACACGGCCAAAGGCGCGGTAGAGACCTTCA TGAAGCTCCGTGCCCGGGACCCTGCCAGCAGAGGAGACAGGTATATGCTGGTCACTTTCGAAGAGCCGCC CTATGCTATCAAGGCTGGATGGAAAGAAAACCATGCAACGTTTATGAATGAACTGAAAAACCTTCAGGCA GAAGGACTTACGACTCTTGGCCAATCCCTAAGGACAGCTTTTGATTTGTTAAATTTAAATAGATTAGTAA CTGGCATAGACAACTATGGGCAGGGAAGAAACCCCTTTTTCTTGGAGCCAGCAATAATAATTACAATCAC TGATGGGAGCAAGTTGACTACTACTAGTGGTGTCCAGGATGAGCTTCATTTACCTCTGAATTCGCCGTTG CCTGGAAGTGAATTGACCAAGGAACCTTTTCGTTGGGATCAGAGACTATTTGCTTTAGTGCTGCGGTTGC CTGGCACCATGTCGGTGGAATCAGAACAGTTGACAGGTGTGCCTTTGGATGATTCTGCTATCACACCAAT GTGTGAAGTGACAGGAGGCCGGTCATACTCTGTATGTTCTCCAAGAATGCTTAATCAGTGTCTGGAATCC TTGGTGCAGAAAGTACAAAGTGGGGTGGTAATAAACTTTGAAAAGGCAGGACCAGATCCTCCCCCTGCTG AAGAGGGGCAGCCAGATATATCAAGACCTTTTGGATCTCAGCCTTGGCATAGCTGTCACAAGCTCATATA TGTCAGACCAAATCCTAAAACTGGGGTTCCTATAGGCCATTGGCCTGTTCCAGAGTCTTTTTGGCCAGAT CAGAATTCTCCAACACTACCACCTCGTACATCTCATCCTGTAGTGAAGTTTTCCTGTACAGACTGTGAGC CAATGGTTATTGACAAATTGCCTTTTGACAAATATGAGTTGGAACCTTCACCACTGACTCAGTTTATTCT AGAAAGGAAATCTCCTCAAACGTGTTGGCAGGTGTATGTGAGCAATAGTGCAAAGTATAATGAACTTGGC CATCCCTTTGGTTACTTGAAAGCTAGTACAGCACTGACCTGTGTCAACTTATTTGTGATGCCTTACAATT ACCCAGTCCTCCTTCCTCTATTAGATGACTTGTTCAAAGTGCACAAAGCAAAACCAACTCTGAAATGGAG ACAGTCATTTGAAAGTTATTTGAAGACAATGCCTCCCTACTATCTTGGGCCCCTGAAGAAAGCTGTTAGA ATGATGGGTGCACCTAATCTGATAGCAGACAGTATGGAGTATGGACTCAGTTACAGTGTCATTTCATACC TCAAAAAATTGAGTCAACAGGCCAAAATTGAATCTGATAGAGTCATCGGATCCGTAGGCAAAAAAGTAGT ACAGGAAACTGGAATTAAAGTCCGAAGCCGGTCACATGGGTTGTCAATGGCGCATAGGAAAGGTTTTCAA GTGCTGCAGGGTATTTCGGAGGATGTCCCCCACAGGTTGCTAGACCTTAATATGAAGGAGTACACTGGGT TCCAGGTTGCTTTACTAAATAAGGATTTGAAGCCTCAGACCTTTAGAAACGCTTATGATATACCAAGACG GAACCTTCTGGATCATTTAACAAGAATGAGATCGAACCTTTTAAAGAGCACCCGCAAATTTCTTAAAGGC CAGGATGAAGATCAAGTGCACAGTGTACCTATAGCACAAATGGGGAATTACCAGGAATACCTCAAGCAAG TACCTTCTCCATTGAGAGAACTTGATCCTGATCAGCCTCGAAGGTTGCATACATTTGGCAATCCCTTTAA ACTGGATAAAAAGGGTATGATGATAGATGAAGCAGATGAATTTGTGGCTGGACCTCAGAATAAACACAAG CGGCCTGGAGAGCCAAGCATGCAAGGGATCCCAAAAAGACGCCGGTGTGCCTCTCCACTCTTGAGAGGCC GAAGGCAGAGCCCTGCTGTGAACAGTCACATCGGAGGGAAAGGACCACCTGCACCCATGACGCAAGCACA GCCAGGTCTTATTAAACCCCTTCCTCTGCATAAAGAAGCCACTAATGATTCAATAGTAGATGATGTGGTT GAAAATCATGTTGCTGACCAACTTTCATCAGACATGACACCAAATGCTATGGATACTGAATTTTTAACAT CTCCCCCTAATCTTTTGGAACCATCAACCAATCATACAGAGGCCCTTGGTCATGAACATTTAGGAAACAA TGACCTCACTGTTGGTGGGTTTTTAGAAAATCATGAGGAGCCAAGAAATAAAGAACAAAGTGCCGAAGAG AATATACCAGCATCTTCACTCAACAAAGGAAAGAAATTGATGCATTGTAGAAGCCATGAAGAGGTCAACA CTGAACTAAAAGCACAAATAATGAAAGAGATCAGAAAGCCAGGAAGAAAATATGAAAGAATCTTCACCTT ACTGAAGCATGTGCAAGGCAGTTTACAAACAAGGCTAATATTCTTACAAAATGTCATTAAGGAAGCATCC AGGTTTAAGAAACGAATGCTAATAGAACAACTGGAGAACTTCTTGGATGAAATTCATCGAAGAGCCAATC AAATCAACCATATTAATAGCAATTAAAAGAAAATAGAATGAGGCCACTTATTTCATTGTCTTCTCCAAAT ACAAAATTGGGCATCCTTCCACTGGCCTGTGAGCAGAAAGCCCTGATTTCCTCCGTTTTCCGAGGCTGGT GACAGGCACTTGGCTTGGACGACCACACCCAAAACACTCACGGAGGAGAGAACCGAAGAGAATGGGGAGA AAAAGCTATATCCCATATTTTAAAAAGAAAGTGAGGAAATATCCTTGGGGAAACTGCTGCACTTTATGAT AACCAGGAGGCTCTCCAAGGAGGAGCAAGACTCACTTTGGATGTGTGAGTTACATAGTTGGGGTATTACT TGAGTGTTTCCACAAAGCTTCTTCTTGTGTTGGAAGCTTGGCTCCCTGGGTAAGTGGTATTGAGTGGGTG GGGCCTTTGGAAAGTTGGGCCTAATAGAAGGTGACCAATCTGGACAGCCCTTGGGAGGTTTAAACATAGT TCTGCTGTTGGTTCTCATGAGAGTGGGTCTCTATGCAGACCCTAAATTCTGTCACATCCCTTCTTGCTCT GTGGCTGCTTCTGCCACTGTGATGCCACCATCTGTGTCCTGATACAGAGCAGAGAGACTTTCAGAACTGT GAGCTGAATAAAATGTGTATATTATATAATGTGTGTGTGTGTGTGTATATACTATATTCTAACATATTAG GGAATAAACTGAAAAGCAAATTGATTTATATCTGGATTGATACCTTGGACCATGTTAGACACAGCCAGGG TTTTGTTGTTTGTCTTTTAGACAGGGTCTCACTATGCAGCCTTGGCTAGCTTGGGTTGTTCTATATTTCT AAACATACTGCTTTCTAACTTCAAACATTGTTTTTCCAACCAATAAAATGCAGGCTAGTACAATATCATA ATCTAATTTTGTGAATGTTTTCAGCTTTCTCCTTTCTCTACCCAGAAGCAAGCATTTTATTCATTGAGTA TATTACTTGACAGTGTTAATCTTCCCTGGACTATGGCTGTGATTGTAGGATTATGGATACAAATGTCCTA TAAACACCCATTCTTGATTATTTAACAGATTACATGTTCTTAATGAAAACTAACTATCCTTGTGCATTTA ATTTTATAAAAAATAATTACTCTGGAAGATGGCTTCATAGGTCACCTCAGAATACTGCTTGGTGGTGTTC AAACTCAAGCTCACCCTCTGGGCTCGTGCCAAGTAACATGTAACATTGCATTGGTCTCTACATTGGGCTG CCACTCACTGAGACCTGTATGATGAGCACAGAGCACGGAAAATGAATAATTTGAGAGAGCTAGAGGTAAG TTCTTACTCAAAAGCTGGCTGTGGAGTGGGATGTAGTAACCCCCACTTTTAATCCTGCCACAAAAGGCAG AGGCAGGAGGATCTCTGTGAGTTTGAGGCCAGCCTAGTCTACATAGCAAGTTACAGGCTAGCCAGGGCTA CATAGTGAGCCTCTGTCTAAGGAGCAAACAAGCAAGTGAACGAACAAACTCTTGATCATATCTACTGTGG TTCAAGCTATATGTCTAAATCCAAACCTAAAGCAGTATTAGCTTTTCAGATTAGTCCCTCAAACTTCCTA ATGCTGTGACCCTTTAACACAGTTCTTCACGTTATGGTGACCTCCAACCATAAAATTATTTTTGTTGCTA CTTCAAAACTGTAATTTTGCTACTATTATGAATCATAATGTAAATATCTGCTATGCGATCCCTAAGCAGT CATGACCCACAAATTGATAACCACTGCTTAAAGAATTTATATCTTGGCTTTTTGTTTGATTGGGTTTTTT TCTCTTTGGTCTGGTCTGGAGTGACTGAGTGAGTGTGTGTTTCTGTTTGTGTAAGATTCGTTTTGCTTAT GAAATAAATGGCTTTATTTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409580 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409580 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14566 same embryo
	EMAGE:14567 same embryo
	EMAGE:14564 same embryo
	EMAGE:14565 same embryo
	EurExpress:euxassay_005414 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source