EMAGE mouse gene expression spatial database

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EMAGE:14566

2700050L05Rik RIKEN cDNA 2700050L05 gene ( MGI:1919831)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005408_01

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14566_wholemount_moderate.wlz

14566_wholemount_weak.wlz

14566_wholemount_possible.wlz

14566_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14566_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8794
Entity Detected:	2700050L05Rik, RIKEN cDNA 2700050L05 gene ( MGI:1919831)
Sequence:	sense strand is shown >T8794 GCTGAGTGTCCGAGTCGCTCGCTCTGAGGTTCTCCTGCGACTCTGGGCCCGCGTTCCTGTCGCCGCCTCC AGGGCTCGCGCGTCTGGTCGCCTCCCCAGGGTCGGAAATGATGGGGGACCCCAAGGAAGCAGGAGCCGAG GCTTCTCCTTCAGGGGCGGCCGCCCGAGGAGGGCTCAGCCTCCTGTCCCAGGCGGACTCCGAGGAGCCTT CTGCACAGGGATCGGCCTTATTTCTTGGAGGCAACGAAGTGAAAAGCAGAGCTGTGGTAAAATACTCTTC TGCCCCTCCTCGAACAGCGTTTGCACGTCTCGAAGAAAAAACAGACTTGAAACTCCCACCTGCCAACTGG TTACGAGAGAGTGCCAAACTAGGGCCAGCAGGAACCACCATTCTTGGCAATAGTAAGAAAAGCAAGCCGT TTTCAAGTTTCGGCATGGCGTATGACTTCATTGACTCGGTGGGAAATGATGTGGATGTCGTCTCCGACTC AGAAAACATAAAAAAGCTCCTGAAGATTCCCTACAGCAAGTCCCACGTGAGCATGGCTGTGCACCGCATA GGAAGGACCCTCTTACTAGATGAGCTGGACATTCAAGAACTCTTTATGAGGTCATCTCAGACTGGTGATT GGACATGGTTGAAAGAGTTCTATCAAAGACTGATCGATCAGAAGTGGCAGAGGAAGAAGAAAAGCAAGGA GCACTGGTATCAGAAGGCTATTCTTTCCAAGTTCTTGTACTACAGCATCAATGGTGATGGAGCTGCGCAG CCTGTCCCGTCTCCTGCAGAACAAGAAGAGTCATCGAGTTCTCAGCAGACACATGAGTCTGAAGGGGCCG CCTGGCCTGCTCCCTTTGAAATGCCCTCTTCCGTGTCTGAGGATCCCAGTGCTTCCAGTCAGGGCAGGGA GCCTCTTGAGCCCTCATGCATAGTGGGGCATGTGGCCTCAGCACCTAAAGAGCAAAACCTTACTACTTTG TTCAATGACGGGGAGAACAGTCAGGGTCTTAAAAATGATTTCGTCCGGAATATCCTGTGGACTTTTGAAG ATATTCATATGCTAGTTGGGTCTAACATGCCTATATTTGGTGGAGGCAGATATCCAGCAGTTAGCTTACG TCTCAGGGATAACAACAAACCGATTAACGTGTTAACTGGAATAGACTATTGGTTGGACAACCTGATATGC AATGTTCCAGAGCTCGTGATGTGCTTCCATGTTAACGGGATCGTACAGAAATATGAAATGATAAAGACAG AAGAAATCCCCAATTTGGAAAACTCTAATTTTTCTACCAAAGTTATAAAAGACATTGCACAAAATATTTT ATCATTTCTTAAATCTAATTGTACCAAAGAAGGGCATACCTATTGGCTCTTTAAAGCAAGCGGCAGTGAT ATTGTGAAACTCTACGACCTCACGACTCTGTGTGAAGAAACTGAAGACAAGTACCAGAACCCATTCACAA TGCCAGTGGCTATCCTCTTATACAAGGTTGCTTGTAACATGATGATGAAGAAAAATCAAAACAAGAAACA CTATGGAACTATTAGAACATTGCTTCTCAACTGTGTTAAGTTGTTGGACAAAAGCAGACATCCTCAAATT ATTGCTTCAGCCAACTACATGCTTTCAGAGCTTTTCCAGCTGGATGAGCCTAAAAAGGAGGAAAGTTCAG ACTCCCCTTTAAATGAGAATTCGGATGAAAGCTACAGTGAAGAGGAGGAGGAGATGGCAGACAGTGATGA GAATGGATCCTACAGCACCAGCTCTGACCCAGCAGATGACAACAAGGCAGTGGCTATCATTAAGTCTGTT GGAGAACTGTCAGTACCGGAAAAATACAAGTCGATTCATCAGATCAGACCCAGTTGTGCATTTCCCGTTT GCCATGACACAGAAGAGCGCTGTCGACTCGTCCTTAGCTATGTGTTGGAGGGTTTAAAATCTGTAGATAG TAGCATCAAAAAAGAAAGCGACCTTCCAGCAGCTGACCCCAGCACTCCTATCCCCTTAAAATATGAAGAT GAATCCACAAGGGGTGGCCCTGAGGGGCTGGAGAAGCAGATGGCCTTGTTCCTGGACAAAATGGGCTCCA TTCAGAAGGGCAGTTGTTCTGGGCAGTCTGGAATGACCCCTGGTTCTTGGCAACATAAAATGAAACTTCA GCTGATTCTCAAGTCATCAAAGGCCTACTACATTTTGTCAGATGCTGCCATGAGTCTTCAGAAGTATGGA AGAGCATTGAGATACATTAAGCTAGCTCTACAGAGCCATGATACTTACTGCTGCCTCTGCACCAACATGC TTTCTGAAGTGCTGCTGTTCCTCTCTCAATACCTGACACTTTGCGGCGACATCCAACTCATGCTGGCCCA GAATGCAAATAACCGAGCAGCACACCTCGAGGAGTTTAATTACCAAACAAAAGAAGACCAGGAGATCCTC CACAGTCTCCACAGAGAGTCCAGCTGCCAGGGATTTGCATGGGCAACAGATCTATCTACAGACTTAGAAA GTCAACTCTCAGTTAGTTGTAAATGTTATGAGGCTGCTAATGAAATCTTGCAATTCAGCGACTTAAAAAG TCAGAATCCAGAACACTATGTACAGGTGTTGAAGAGAATGGGAAACATTAGAAATGAAATCGGTGTATTC TACATGAATCAGGCTGCTGCACTGCAGGGTGAGCGAGTAGTGAGCAAATCTGTGTCTGCTGCAGAGCAAC AATTGTGGAAAAAAAGCTTTTCTTGTTTTGAAAAGGGGATTCACAACTTTGAGTCGATTGATGATGCTAC AAATGCTGCCCTTCTGCTGTGCAACACGGGAAGACTCATGCGGGTCTGTGCGCAGGCGCACTGCGGTGCA GAGGATGAGTTCAAACGGGAGTTCTCTCCAGAGGAGGGCTTGTATTACAGCAAGGCCGTTGATTACTATT TGAAGGCTCTGAGGTCATTGGGAACGAGAGACATGCACCCAATTGTTTGGGATTCTGTGAACTGGGAATT GTCCACTACATACTTCACCATGGCGACTCTGCAGCAAGATTATGCTCCATTATCCAGAAAAGCTCAGGAG CAGATTGAGAAAGAAGTGAGCGAGGCTATGATGAAGTCCCTGAAGCACTGCGATGTTGACTCAGCAACTG CTCGGCAGCCCCTCTGTCAGTACCGAGCTGCGACCATCCATCACAGGCTGGCTTCCATGTACCACAGCTG TCTGAGGAACCAGGTTGGTGATGAGCATCTTAGGAAGCAGCACCGAGTGCTGGCAGATCTCCACTACAGC AAAGCTGCGAAGCTCTTTCAGCTTCTCAAAGACGCTCCCTGTGAACTGCTTAGAGTGCAGCTAGAAAGGG TGGCATTTGCAGAATTTCAGATGAGCAGTCAGAATAGCAATGTTGGAAAGTTAAAAACATTATCTGGGGC ACTTGATATCATGGTGAGAACGGAGCATGCGTTCCAGCTCATCCGGAAGGAGCTTGTAGAGGAATGTGAC CAGCCCAAGAATGATGAGGCTACACCGGCTGCTGATTCTTCACCTAATCTTAATCGAGAAGAAGTGATCA AACTCCTTAGTATATTTGAATCTCGTCTGTCATTCCTTCTCCTTCAATCCATCAAACTGATGTCGTCATC TAAAAGGAAAATGAGCAGTAACGCTGAAGAAGACATAGTCCTCCAAACCAACAAGCAGATTTACTCCCAG CTTTTGAGAGCGACTGCCAACAGAAACTCAACTCTGCTGGAAAGAATTGAAGTCGTCATCTGTTTGCTGG AGCAGCTTGCCTCCGGTAGCAGCCGATCGAGTGGCAGTGCTGTCCCCTGACTGCAGTGGTACTGGAGGAC TGTCTTGTCAGTGCCTTCCCGTCCTGGAGCTGTCTGTCTGTCCACTCGGTGCTGAGACTTATTAAATATG AGGACATAGCGTTTAATCAGGTCCACAGTGGATACTTGCTTCCCATGGCAGGCAGAGGATGTGGGGTGGT TCGGCTTCTTGTGAAGGTGAGAAACTATAATGGAACCGAAAGCTTTGTAAATGTATTTCCTCGAGAAGTA CATGGTTCCTTAGTGATAACACCTCCTGCGATGTCGCGAGACTTAAAATATTCTTGACGTTAAAACACTT AGGTGTAAGATGCGATTATTTTGGATAAAATGTTACTCTGGCCAAGGGAACTCTTATTCAAGTGATTTCA TGGATTCAAATGGCATGTAGAAACAGTGCGCATCAGCGTCTACTTCTAAGTGACACAAAAGTGTCCCCTT TCCAAGCTGTGGTGTGCGCGGGAGGTTTTGACGACTGAGAATTGTGCAGGTCTCCTTTCAGTGCATGCTC TGAAGAGACTCCTTCTCTGACTGTGTATGACATTGGTAAAGAATAGGTACTATCACTTTCTCATTTTAAA CTTGAAACTGTGAATAAGGTAAGAGAATTATTTTGAATAAATAAGCTATTTATTTAAAATACCTTGGTAG TTCCCACTTGCATTCTTCACACTGCTGTGATTTGATCTGCTTGCTTATGATATCAAACCTTTACAAAGTA CATGGCTCCTAATTTTCAAGGCAAAGGTTTCCCCTACATTCCTGCTCCTGGGACATTCTGCTGTGCCCTG CTGCTCTGGGGATCTTGTCCAGTGCCCACTGCATGTGGGGCCGTCAAGCTGTCACTAACAGTTGCAAGGA AGTCCAGCATTTGCACCAGGCAGACTCTTGATATCTGGTATCTGGCCACAGTTACATCCCCTTCCACACC AGCCCCACTTACAGATGTGGGTTTCTGTCACTGGAGCAAGCAGGTCTCCATTGCAACAAAGCAGATTATT CTCTCACTATTTGTCTAGTTCCTTGTTTGGTTTGCATTGTTGTCATTGGAACAAATTTGCACCTGTTTAC AAGAGTTAATCATTGCAACCCTTAAAACGTTGGTGAAACTGTAAAGCTTTGAGCCAGCTCCTCAGCCTCC TTTTAGGACGCCTGTGTCCTTAGCCCTGCAGCAAGTGTGCACACAGTACCACAGCCCTTCCTTCCTCCAC TGGAGGGCCCTGTGGAACAGGCGAACTCATCAGGATTCCCGTGCTGCCCTAGAAAGAGGCTGGAGTGTTA GGACTACTTGTCTCCAAGCCCCTCTCCTTAGCTGAAAAGCCTTCTGATAGGAAAATGGATTTTAAGCAAG GAAGTAGAAATTTTGGCTCTTTCTCTTGTCATAGCAATAACATGAAAATTTCTGCAGGAGAAATAAAAGA TACTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409214 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409214 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14563 same embryo
	EMAGE:14567 same embryo
	EMAGE:14564 same embryo
	EMAGE:14565 same embryo
	EurExpress:euxassay_005408 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source