EMAGE mouse gene expression spatial database

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EMAGE:14599

Aebp1 AE binding protein 1 ( MGI:1197012)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005502. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005502_01	euxassay_005502_02	euxassay_005502_03	euxassay_005502_04

euxassay_005502_05	euxassay_005502_06	euxassay_005502_07	euxassay_005502_08	euxassay_005502_09

euxassay_005502_10	euxassay_005502_11	euxassay_005502_12	euxassay_005502_13	euxassay_005502_14

euxassay_005502_15	euxassay_005502_16	euxassay_005502_17	euxassay_005502_18	euxassay_005502_19

euxassay_005502_20	euxassay_005502_21	euxassay_005502_22	euxassay_005502_23	euxassay_005502_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14599_wholemount_strong.wlz

14599_wholemount_moderate.wlz

14599_wholemount_weak.wlz

14599_wholemount_possible.wlz

14599_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14599_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9600
Entity Detected:	Aebp1, AE binding protein 1 ( MGI:1197012)
Sequence:	sense strand is shown >T9600 ACGCGGGGCAGGAGCGCCCAGTTGCTGCCGGAGCTGGGCCCGCCAGAACCTCTCCTGGAGCCCCTTGCTC TCCTTGAATCTCCCTTTCCCACCGCTTTCTGGATACCCTTGACGCCCACGTTCCTCGCGCCCTTTCCCGC CCCTACGCGGGGCGCTGCCCCTGCCACCCAAGTCCCTGCTCAAGCCCGCCCGGCTCCCGCGCGTGCCCAG AGCCATGGCTCCAGTGCGCACCGCATCCCTGCTCTGCGGCCTCCTGGCACTGCTGACGCTGTGCCCTGAG GGGAACCCACAGACGGTGCTGACGGACGACGAGATCGAGGAGTTCCTCGAAGGCTTCCTTTCGGAGTTGG AGACCCAGTCCCCGCCCCGGGAAGACGACGTGGAAGTCCAGCCGCTTCCCGAACCCACCCAGCGTCCCCG CAAATCCAAGGCAGGGGGCAAGCAGCGGGCAGATGTAGAAGTCCCTCCAGAAAAAAACAAAGACAAAGAG AAGAAAGGAAAGAAGGACAAAGGCCCCAAAGCCACAAAACCCCTGGAGGGCTCTACCAGGCCCACCAAGA AACCAAAGGAGAAGCCACCCAAGGCCACCAAGAAGCCCAAGGAGAAACCACCCAAGGCCACCAAGAAGCC CAAGGAGAAGCCACCCAAGGCCACCAAGAAGCCTAAGGAGAAGCCACCCAAGGCCACTAAGAGGCCCTCG GCAGGAAAGAAGTTCTCAACTGTGGCCCCCTTGGAAACGCTGGATCGGTTACTCCCCTCACCCTCCAACC CCAGCGCCCAGGAGCTACCGCAGAAGAGAGACACACCCTTCCCAAATGCCTGGCAAGGTCAAGGAGAAGA GACCCAGGTGGAGGCCAAGCAGCCCCGGCCAGAGCCAGAGGAGGAGACTGAGATGCCCACACTGGACTAC AATGACCAGATAGAGAAGGAGGATTACGAGGATTTTGAGTACATCCGTCGCCAGAAGCAGCCCAGGCCAA CACCCAGCAGGAGGAGGCTCTGGCCAGAGCGCCCTGAGGAGAAGACTGAAGAGCCAGAGGAAAGGAAGGA AGTCGAGCCACCTCTGAAGCCCCTGCTGCCTCCGGACTATGGGGATAGCTACGTGATCCCCAACTATGAT GACTTGGACTATTATTTCCCCCACCCTCCACCGCAGAAGCCTGATGTTGGACAAGAGGTGGATGAGGAAA AGGAAGAGATGAAGAAGCCCAAAAAGGAGGGTAGTAGCCCCAAGGAGGACACAGAGGACAAGTGGACCGT GGAGAAAAACAAGGACCACAAAGGGCCCCGGAAGGGTGAGGAGCTGGAGGAGGAGTGGGCGCCAGTGGAG AAAATCAAGTGCCCACCTATTGGGATGGAGTCACACCGCATTGAGGACAACCAGATCCGTGCCTCCTCCA TGCTGCGCCACGGCCTCGGAGCCCAGCGGGGCCGGCTCAACATGCAGGCTGGTGCCAATGAAGATGACTA CTATGACGGGGCATGGTGTGCTGAGGACGAGTCGCAGACCCAGTGGATCGAGGTGGACACCCGAAGGACA ACTCGGTTCACGGGCGTCATCACTCAGGGCCGTGACTCCAGCATCCATGACGACTTCGTGACTACCTTCT TTGTGGGCTTCAGCAATGACAGCCAGACCTGGGTGATGTACACCAATGGCTACGAGGAAATGACCTTCTA TGGAAATGTGGACAAGGACACACCTGTGCTGAGCGAGCTCCCTGAGCCAGTTGTGGCCCGTTTCATCCGC ATCTATCCACTCACCTGGAACGGTAGCCTGTGCATGCGCCTGGAGGTGCTAGGCTGCCCCGTGACCCCTG TCTACAGCTACTACGCACAGAATGAGGTGGTAACTACTGACAGCCTGGACTTCCGGCACCACAGCTACAA GGACATGCGCCAGCTGATGAAGGCTGTCAATGAGGAGTGCCCCACAATCACTCGCACATACAGCCTGGGC AAGAGTTCACGAGGGCTCAAGATCTACGCAATGGAAATCTCAGACAACCCTGGGGATCATGAACTGGGGG AGCCCGAGTTCCGCTACACAGCCGGGATCCACGGCAATGAGGTGCTAGGCCGAGAGCTCCTGCTCCTGCT CATGCAATACCTATGCCAGGAGTACCGCGATGGGAACCCGAGAGTGCGCAACCTGGTGCAGGACACACGC ATCCACCTGGTGCCCTCGCTGAACCCTGATGGCTATGAGGTGGCAGCGCAGATGGGCTCAGAGTTTGGGA ACTGGGCACTGGGGCTGTGGACTGAGGAGGGCTTTGACATCTTCGAGGACTTCCCAGATCTCAACTCTGT GCTCTGGGCAGCTGAGGAGAAGAAATGGGTCCCCTACAGGGTCCCAAACAATAACTTGCCAATCCCTGAA CGTTACCTGTCCCCAGATGCCACGGTCTCCACAGAAGTCCGGGCCATTATTTCCTGGATGGAGAAGAACC CCTTTGTGCTGGGTGCAAATCTGAACGGTGGTGAGCGGCTTGTGTCTTATCCCTATGACATGGCCCGGAC ACCTAGCCAGGAGCAGCTGTTGGCCGAGGCACTGGCAGCTGCCCGCGGAGAAGATGATGACGGGGTGTCT GAGGCCCAGGAGACTCCAGATCACGCTATTTTCCGCTGGCTGGCCATCTCATTTGCCTCCGCCCATCTCA CCATGACGGAGCCCTACCGGGGAGGGTGCCAGGCCCAGGACTACACCAGCGGCATGGGCATTGTCAACGG GGCCAAGTGGAATCCTCGCTCTGGGACTTTCAATGACTTTAGCTACCTGCACACAAACTGTCTGGAGCTC TCCGTATACCTGGGCTGTGACAAGTTCCCCCACGAGAGTGAGCTACCCCGAGAATGGGAGAACAACAAAG AAGCGCTGCTCACCTTCATGGAGCAGGTGCACCGTGGCATTAAGGGTGTGGTGACAGATGAGCAAGGCAT CCCCATTGCCAATGCCACCATCTCTGTGAGTGGCATCAACCATGGTGTGAAGACAGCAAGTGGAGGTGAC TACTGGCGCATTCTGAACCCGGGTGAGTACCGTGTGACAGCTCACGCAGAGGGCTACACCTCAAGTGCCA AGATCTGCAATGTGGACTACGATATTGGGGCCACTCAGTGCAACTTCATCCTGGCTCGATCCAACTGGAA GCGCATTCGGGAGATCTTGGCTATGAACGGGAACCGTCCCATTCTCCGAGTTGACCCCTCACGACCCATG ACCCCCCAGCAGCGGCGCATGCAGCAGCGCCGTCTACAGTACCGGCTCCGCATGAGGGAACAGATGCGAC TGCGTCGCCTCAATTCTACCGCAGGCCCTGCCACAAGCCCCACTCCTGCCCTTATGCCTCCCCCTTCCCC TACACCAGCCATTACCTTGAGGCCCTGGGAAGTTCTACCCACTACCACTGCAGGCTGGGAGGAGTCAGAG ACTGAGACCTATACAGAAGTAGTGACAGAGTTTGAGACAGAGTATGGGACTGACCTAGAGGTGGAAGAGA TAGAGGAGGAGGAGGAGGAGGAGGAGGAAGAGATGGACACAGGCCTTACATTTCCACTCACAACAGTGGA GACCTACACAGTGAACTTTGGGGACTTCTGAGACTGGGATCTCAAAGCCCTGCCCAATTCAAACTAAGGC AGCACCTCCCAAGCCTGTGCCAGCAGACACATAGCCATCAGATGTCCCTGGGTGGACCCCACTCCCCCAG TGTGGGACATCAAAGCTACCGGGACTCTGCATAGACTCTGGTCTACCCGCCCCAGCTCTACCTGCCAGCC TTTGGGGAGGGGCAGGCAAGGAAGCCAACGTCACATCAATAAAACAAGCTCATGACACCACCAAAAAACA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30648530 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30648530 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14602 same embryo
	EMAGE:14603 same embryo
	EMAGE:14600 same embryo
	EMAGE:14601 same embryo
	EMAGE:14598 same embryo
	EurExpress:euxassay_005502 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source