EMAGE mouse gene expression spatial database

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EMAGE:14602

Antxr2 anthrax toxin receptor 2 ( MGI:1919164)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005503. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005503_01	euxassay_005503_02	euxassay_005503_03	euxassay_005503_04

euxassay_005503_05	euxassay_005503_06	euxassay_005503_07	euxassay_005503_08	euxassay_005503_09

euxassay_005503_10	euxassay_005503_11	euxassay_005503_12	euxassay_005503_13	euxassay_005503_14

euxassay_005503_15	euxassay_005503_16	euxassay_005503_17	euxassay_005503_18	euxassay_005503_19

euxassay_005503_20	euxassay_005503_21	euxassay_005503_22	euxassay_005503_23	euxassay_005503_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14602_wholemount_strong.wlz

14602_wholemount_moderate.wlz

14602_wholemount_weak.wlz

14602_wholemount_possible.wlz

14602_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14602_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9599
Entity Detected:	Antxr2, anthrax toxin receptor 2 ( MGI:1919164)
Sequence:	sense strand is shown >T9599 CAGCTAGACGCCAGCCTTTCTGCTCCCGGGTGGGCGCCGGAGACTCAGCGGGAGGCGAGGAACTTGTCCC GAGAGCTGTCGCTGCGGGCCTCATTGTCTGCAGCAACTCTCCGGAATCCGGAGTGGGAGAACTGGACTCT GTCCACTGGGATTTGCCCAGAGTTCCGGCGGCAGCTGTGGCGGCTGCGGAGACTCTCTTTGTCCTCCCAG GACCTCGCTCTCTCTCCTCAGTGGCAGCTGTTGGGTGGCTCTGAGCTTGGAGAGGGCATCCCAGCCCCGC TCAGCCTCTGACCCCCGGGCGAGTCCCCGGTGACTCCGCCTCTTTCACGTCTCTGCTCTGTGAAGCCCGG AGCCCAGAACCCCGAGCCCAAGGGACTGTGAGCCAGAGAGAGCTGCCGGGCCCCGGCCACAGGATGGTGG CCGGTCGGTCCCGGGCGCGCAGCCCTGGGAGCTGGCTGTTCCCTGGCCTGTGGTTGTTGGCTGTGGGCGG TCCGGGGTCGTTGCTGCAAGCCCAGGAGCAGCCCTCTTGCAAAAAAGCCTTCGATTTGTACTTCGTACTG GACAAGTCTGGCAGTGTAGCAAATAACTGGATTGAAATTTATAATTTTGTCCACCAGCTGACAGAGAGAT TTGTGAGCCCTGAAATGAGATTGTCCTTCATTGTGTTTTCTTCCCAAGCAACCATTATTTTGCCATTAAC TGGAGACAGGTACAAAATTGGCAAAGGACTGGAGGATTTAAAGGCCGTTAAGCCAGTTGGAGAAACATAC ATCCATGAAGGACTAAAGCTTGCAAACGAACAAATTCAAAATGCAGGAGGCTTAAAAGCCTCCAGTATCA TAATTGCTTTGACGGACGGTAAGCTGGACGGCCTGGTACCATCTTATGCAGAGAACGAGGCAAAGAAGTC CAGGTCACTTGGCGCTAGTGTTTACTGCGTTGGGGTCCTTGATTTTGAACAAGCTCAGCTGGAAAGAATT GCTGATTCCAAGGACCAGGTTTTCCCTGTCAAAGGTGGATTTCAAGCTCTCAAAGGCATCATCAACTCTA TATTAGCTCAATCATGTACTGAAATCCTGGAATTGAGTCCTTCAAGTGTCTGTGTAGGGGAGAAATTTCA AGTTGTTCTGACTGGAAGAGCAGTCACGTCGATCAGTCACGATGGCAGTGTCCTCTGTACATTCACTGCA AACAGCACATATACAAAGAGTGAGAAGCCAGTGAGCATTCAGCCAAGTTCCATCCTTTGTCCTGCACCTG TCCTGAACAAAGATGGAGAAACTCTTGAAGTTTCAATCAGCTATAATGATGGGAAGTCTGCTGTCTCAAG ATCCTTAACAATCACAGCCACAGAATGTACCAATGGGATTGCAGCCATCGTAGCTATTTTGGTGTTGTTG CTGCTCTTGGGTGCTGCCTTGATGTGGTGGTTTTGGCCCCTTTGCTGCAAAGTGGTTATCAAGGACCCTC CCCCACCACCTTCTGCACCAATGGAGGAGGAGGAGGAGGATCCTTTGCCCAACAAGAAGTGGCCGACTGT GGACGCTTCCTACTACGGAGGTCGAGGTGTTGGAGGAATTAAAAGGATGGAGGTCCGCTGGGGAGATAAA GGATCTACAGAGGAAGGTGCAAGGCTAGAGAAAGCAAAAAATGCTGTGGTGATGGTCCCTGAAGAAGAGA TCCCCATCCCATCCAGACCACCTCGACCCAGACCCACACACCAGGCACCTCAGACAAAGTGGTACACGCC AATCAAGGGTCGTTTGGATGCACTCTGGGCTTTGATCATGAAGCAGTATGACCGGGTGTCCTTGATGAGA CCCCAGGAAGGTGATGAGGGCCGATGCATAAACTTCTCCCGGGTTCCACATCAATAAGACGGGAGAACAA AGAATGAGAAGATAAGAAGACAGTGTGACAGTGTATCTTCATGATGCTGATTTCCAACAGAACCGACAGT CCGGTGCATCTCAGAAGTTCTTGGGACAACAGCCCATTTTCTCTCTGTCAGGAAATGTTTCCTCTGCCTT CTGCTTTCTGTGCACTAAACATTTTCCAACACTTGTTCTGCCATCGACATGAGAGGTGATGAAAGTCATC GGCGATAGCCCATGATTCACGACACTGAAAATCCCGAGGAATGTTAGTTTGCATGCTAGGGTTTATGCAA AGCTCGTTTTGACTATGTAAGAGGACAAAGCAGACACATCGATGATGTAATGATACCAAAGCTAGGACTG CAAATCCACCAGCCACAGAGGTTTGCAATGGAGACTGGTGATTCTGCCATGAATGTGTGGCCCCTGTGCT TTTGTTTGGCAAGATCTTTAGCTACAAGCAAAACATGAAGTTTCCTCCCAGGCTAAACAGATAATGGAGT CCACTGCCTTGTAGCTATGTCAGATAGCAAAGCCTTTCCAAGTCCTCCATTACTTTGTGCCTTACAGGAA ATTTCTGACTAGAAAATCTTGTCACTGTTACACTGAAAGTGCACACGCATGACAAAATGTAGACGAGACG CCTCAAGGTACTGGATGCAAGCAGGATTTTTGCCCTTTAGTTTTCCAAGACACCTTTCTTTTATTATGCA CTTGAGACAAGAGAATTAATAGAGCGTTAATTCAACAGGAAGACCGCCTCCAACCAAAGACCTGGAGCGC AGCATAAGGACTTGTGATTTGAGACGTTGTCCCCAGCCTGGTAGATCCCCCTTTCTCAGCATTTGGGATT TAGCAGTGCATAAAGCATTAATATCTGTAAAAACACCTAGATGTTTGTTTGGCTTTTAATTTAAGGAAGC TGCAACCACAAAGCTTCCGCTCAGGGTTTTTTCTTCCTTCAAGTCTCCAAGGGCTCTTCAGCGTCACAAG CCAGCAACTCTCTTTGCATGAAAATTTCAAAGTTTAATTAATATAATTAAAGGCAACAGCAAGCAGCAGC CTGTGAAGATTTTGCTCATCTTTTTTATGCCTTTTGACATTGAGTGACCTATCACTGTATGCATGTTACT TAGAAATTGAGGAGCACCACACCTTGGTTGTGGTTCAGCCTGGGAAAGAGACCTCCTTCCTTCTGTTTAT AAATTAAAATCAGGAGGGGCGCCATCAGAAAGCATGGACAATATACATACTATAAATTTTTAGAAATATC ACCATCGTGTCACGTCAACGATGCCAAATTATGTTAGTGTGAGCAGAAACCCGGTGGGGGAGGAAGGCGG CAGCAGCCGAAGGAAATAGCTCAGATAATCTAGTCACTTTCGATACTGTACTTCAGATGCGAAATGGATA TTCGACTGGAAACCTGACAAAGCGCGCCTGCTTTGATGTGAACTGTTATAGACAATGACCAGTGGCCGGG TCAGTGGGATGTCTCTCTGCGAGCACAAAGGCTTATCAAATGACACTAAAAATAAGTTCAACAACCATCA CTTTGGAAGGGAGAAGGCGAACATTTCATGTTTGGCGGGCATGTGAGTGCAGGAGATGGAAAGAGCCATT TAGAGCATCCTCATATAATTGCCCGCATTGTACTCTTCATGGAAATTTCAAAGGACGGCAGTGATATTTT TCATTGGTGTCCACGTTTGTGGCACTGCTCCAAGAAGCCTTATGCACACATACAAATATACAAATGCACA CACCTACACTCTCTAGCTTTAATCTTTTTGCTCAACCTTATTTATATCACTGACTGGCTGGATCCAAAGT CACACCTCCACATATTAGTGAATAAAAAATTTTTACCTGTGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30649897 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30649897 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14599 same embryo
	EMAGE:14603 same embryo
	EMAGE:14600 same embryo
	EMAGE:14601 same embryo
	EMAGE:14598 same embryo
	EurExpress:euxassay_005503 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source