EMAGE mouse gene expression spatial database

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EMAGE:14628

Slc25a25 solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25 ( MGI:1915913)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005554. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005554_01	euxassay_005554_02	euxassay_005554_03	euxassay_005554_04

euxassay_005554_05	euxassay_005554_06	euxassay_005554_07	euxassay_005554_08	euxassay_005554_09

euxassay_005554_10	euxassay_005554_11	euxassay_005554_12	euxassay_005554_13	euxassay_005554_14

euxassay_005554_15	euxassay_005554_16	euxassay_005554_17	euxassay_005554_18	euxassay_005554_19

euxassay_005554_20	euxassay_005554_21	euxassay_005554_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14628_wholemount_strong.wlz

14628_wholemount_moderate.wlz

14628_wholemount_weak.wlz

14628_wholemount_possible.wlz

14628_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14628_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5431
Entity Detected:	Slc25a25, solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 25 ( MGI:1915913)
Sequence:	sense strand is shown >T5431 CCACGCGTCCGGCGCTCGCTGTCTGAGGAAAGCCGGCGTGCTTCCGCGCTCGGGTCGCTCTCCTGCCGCC TTCAGCCCGGCGTCCGGCGCCCCCGCCTCGCGCCCGATGGTGAGCAGTGTGTTGTGCCGCTGCGTGGCCT CCCCGCCGCCGGACGCCGCCGCCACCGCCTCGTCGTCCGCCTCGTCGCCGGCTTCTGTAGGGGACCCGTG CGGCGGCGCCGTCTGTGGCGGCCCGGACCACCAGCTACGCCTGTGGAGCCTCTTCCAGACACTCGACGTC AACCGCGACGGCGGCCTGTGTGTCAACGACCTGGCGGTGGGGCTGCGGCGCCTGGGACTGCACCGCACGG AGGGCGAGCTCCGGAAAATTGTGCAAGCAGGTGACAAGGACCTTGATGGGCAACTGGACTTTGAAGAGTT TGTACATTACCTCCAAGATCATGAGAAAAAACTGAGGCTGGTGTTCAAGAGTCTGGACAAAAAGAATGAT GGTCGAATCGATGCTCAGGAGATCATGCAGTCCCTGCGGGACCTGGGTGTCAAGATCTCGGAACAGCAGG CGGAGAAGATTCTTAAGAGAATACGGACGGGCCACTTCTGGGGCCCTGTCACCTACATGGATAAGAATGG CACGATGACCATCGACTGGAACGAGTGGAGGGACTACCACCTCCTGCACCCTGTGGAGAACATCCCGGAG ATCATCCTGTACTGGAAGCACTCGACGATCTTCGATGTCGGTGAGAATCTGACAGTCCCAGATGAGTTCA CAGTGGAGGAGAGGCAGACGGGGATGTGGTGGAGGCACCTGGTGGCAGGAGGTGGGGCAGGGGCAGTTTC CAGAACCTGCACTGCCCCCCTGGACAGACTGAAGGTGCTCATGCAGGTCCATGCCTCCCGCAGCAACAAC ATGTGCATCGTAGGTGGATTCACACAGATGATTCGAGAAGGGGGAGCCAAGTCACTCTGGCGGGGCAACG GCATCAATGTCCTCAAAATTGCCCCTGAGTCGGCCATCAAATTCATGGCATATGAGCAGATGAAACGGCT TGTCGGTAGTGATCAGGAGACGCTGAGGATCCACGAAAGGCTTGTGGCAGGCTCCTTGGCCGGAGCCATT GCCCAGAGTAGCATCTACCCAATGGAGGTTCTGAAGACCCGAATGGCCCTGCGGAAAACAGGACAGTACT CCGGCATGCTGGACTGTGCCAGGAGGATCTTGGCTAAAGAGGGTGTAGCTGCCTTCTACAAAGGCTACAT CCCCAACATGCTGGGGATCATCCCCTATGCTGGCATCGACCTAGCTGTCTATGAGACATTGAAAAATACC TGGCTCCAGCGCTACGCAGTAAACAGTGCAGACCCCGGTGTGTTCGTGCTCCTGGCCTGTGGTACTATCT CCAGTACTTGTGGCCAGCTGGCCAGCTACCCACTAGCCCTGGTCAGGACCCGGATGCAGGCACAAGCCTC CATTGAGGGCGCACCTGAGGTAACCATGAGCAGCCTCTTCAAACAGATTCTGCGGACTGAGGGGGCCTTT GGGCTCTACCGGGGGCTGGCCCCCAACTTCATGAAGGTGATCCCGGCTGTGAGCATCAGCTACGTGGTCT ACGAAAACCTGAAGATCACCCTGGGCGTGCAGTCTCGGTGACGGGAGGGTGGTGGACTTGGTGAGCCTGG GCTGCGGCCCAGGGTATGCAGCCACCTCATTCTGTGAATGTGCCAACACTAAGCTGACTTACCCAAGCTG TGAAACCCAGGATACCATAGGGGACGGGCAGGGAGCTGGCAAGCTCTGGGCTGGTTCTGCTGACCTGGCA GACCTTCGTGTCTCTTCCAAGGAAGACCTGTGGATGTTCCTTGGGGTTCAGGGGTCAGTAAGATGTAGGC TCCTGCACTAGAGACAGGACGTTTTCCTCAGTGCCTGCCAGATAGCGAGCTTGGATGCCAGCTTAGTTCT TCCATCTCGTTCACTCAGCCGGACCTCAGCCACGGCATCCCTCTGGTCCAAAGAGCATTCCTGTGCCCCT CATATCTTTCCACTGATGCGCTGTGGTAGAAAGTGGGCCTGCCCCCACTCTTTCTTGTCCTTCTGTCCAT CTTTCCTCAGTGCTGCTCTTCCAAACCTGTTTCCCAAGCTGCAGCATCCATGCGGACCTGTGGTACGGGA AGCTGAAGAAGTACCATTATGTCCTCGCCTATTCTGAGCCCACCAGCAAGGCCACCTGGCTGTGCCCAGG TACAGGCACTGGCTGCCTGACAGCGTGGTGCATGGCTTTGCTGAAAGCTAGTGCTAGGACCTGGAACCCA GAGGACTTGTGCTGCCTGCATGGGCCTAACGTCCTCTGAGCTGCCCCTAAGCCCTGTCAGGACTGGCACC AACTCATGCCCACACTCATTGTTCAGTTCTGTGTGTGAGGGTAGGCAGGGTACCTGCCCCATGTCAGCCG CCCTGATGAGAAGAAAACGATGCTGAAGGCCTTAATTATGTCTTAGTGGGTAAAGGATTTGGTTCAGAAG GACAAGCTGGACACTGCAAGTGCCACACTCGCTGTGAGCCCGGGGCAGGTAGTTTCCCTGCTCTCAAATC TGTTCTGAGAGCCCTGTGGGGCCAAGCGGGACCAGCCTCACATTCCACGTGTGTACCACTCTGCTTGGAG CCTATTTATTTTGTATTTATTTGAACAGAGTTATGTCCTAACTATTTTTATAGATTTGTTTAATTAATAG CCTGTCATTTTCAAGTTCATTTTTTTATTCATATTTATGTTCATGGTTGATTGTACCTTCCTGTCATCAC CTGGTGGGGCATGGGAAGACAAGATGGGAAAGTGGCTACAGAGTGACCTTGCCTGCTGCCCACACTGACA GTGTGTCCGCCCCACCAGAAACAGAGGGAACGGCACAGGGAAGACCACCCAGGCCGGGTAGGGTAGTGGG GAAGGTGGCAGCTTGACATTCTTTCCTGGACCGGGAGGATTTCTTTATTTTGCCCTTTGGAACGACAAGG CAGTGAGGTGCTTCACCAGGAATTTGGTTTGAGGGTGAGGACTGAAGGACAGGGTGGCCTGCCTTCCCCA ACCCGTGACCTGGTCTCCCTCCTGGCCCACAGCTGCCCATGCTCAATGAGTGGCCTGCTGGCCACTTGGC AATTGTGCCTCCATTGCCCCAGAATGGCCTGATGAGGAAAATCCAAACAGGATGCAAAGATCAATGCAAA AATCACCGGTCTGCATAGTCTATGCTGTAACTGGAGTTTGTCAAAGGCAAGCAGCCTTCTAATAAAGTCG CTTCAAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5368306 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368306 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14627 same embryo
	EMAGE:14630 same embryo
	EMAGE:14629 same embryo
	EurExpress:euxassay_005554 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source