EMAGE mouse gene expression spatial database

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EMAGE:14629

Kbtbd2 kelch repeat and BTB (POZ) domain containing 2 ( MGI:2384811)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005543. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005543_01	euxassay_005543_02	euxassay_005543_03	euxassay_005543_04

euxassay_005543_05	euxassay_005543_06	euxassay_005543_07	euxassay_005543_08	euxassay_005543_09

euxassay_005543_10	euxassay_005543_11	euxassay_005543_12	euxassay_005543_13	euxassay_005543_14

euxassay_005543_15	euxassay_005543_16	euxassay_005543_17	euxassay_005543_18	euxassay_005543_19

euxassay_005543_20	euxassay_005543_21	euxassay_005543_22	euxassay_005543_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14629_wholemount_strong.wlz

14629_wholemount_moderate.wlz

14629_wholemount_weak.wlz

14629_wholemount_possible.wlz

14629_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14629_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5428
Entity Detected:	Kbtbd2, kelch repeat and BTB (POZ) domain containing 2 ( MGI:2384811)
Sequence:	sense strand is shown >T5428 ATTAAAGCAGAGTGCAAAGAGGATGGTGGAGCACAAGTTCACGGCTGTGTACCGCCAGGAAGCCTTCATG CAGCTGTCCCACGACCTCCTCATAGACATCCTCAGCAGCGACAACTTAAATGTAGAAAAGGAGGAAACCG TGCGAGAGGCTGCCATGCTCTGGCTAGAGTACAACACAGAGTCACGATCCCAGTATTTGTCTTCAGTTCT TAGCCAGATCAGAATTGATGCTCTTTCAGAAGTCACACAAAGAGCTTGGTTTCAAGGTCTCCCACCCAAC GATAAGTCTGTTGTGGTTCAGGGTCTTTATAAGTCTATGCCCAAGTTCTTCAAACCCAGACTTGGGATGA CTAAGGAGGAAATGATGATTTTCATTGAAGCATCCTCAGAAAACCCTTGTAGTCTTTACTCTTCTGTCTG TTATAGCCCCCAGGCAGAAAAAGTTTACAAGTTATGCAGCCCACCCGCTGATTTGCATAAGGTTGGGACA GTTGTAACTCCGGATAATGATATTTACATAGCAGGGGGCCAAGTGCCCTTGAAAAACACAAAAACAAATC ACAGTAAAACAAGTAAACTGCAGACTGCCTTCCGAACTGTGAATTGCTTTTACTGGTTTGATGCACAACA AAATACCTGGTTTCCAAAGACACCAATGCTTTTTGTCCGTGTGAAGCCATCTTTGGTTTGCTGTGAAGGC TATATCTATGCAATCGGAGGAGATAGTGTTGGTGGAGAACTTAACCGGAGGACTGTAGAAAGATACGACA CTGAGAAGGATGAGTGGACAATGGTGAGCCCTTTGCCTTGTGCATGGCAGTGGAGTGCAGCAGTTGTAGT TCACGACTGCATCTATGTGATGACATTAAACCTCATGTACTGTTACTTTCCAAGATCTGACTCATGGGTA GAAATGGCCATGAGGCAGACTAGCAGATCTTTTGCTTCAGCTGCAGCTTTTGGTGATAAAATTTTCTATA TTGGAGGGTTGCATATTGCTACCAATTCTGGCATAAGACTCCCCTCTGGCACTGTAGATGGGTCTTCAGT AACTGTGGAAATTTATGATGTGAATAAAAATGAATGGAAGATGGCTGCCAACATCCCTGCTAAAAGGTAT TCTGATCCCTGTGTTAGAGCTGTTGTGATCTCAAATTCTCTTTGTGTGTTTATGCGAGAAACCCACTTAA ATGAACGAGCTAAGTATGTCACCTACCAGTATGATTTGGAACTTGACCGGTGGTCTCTGCGGCAGCATAT ATCTGAACGTGTACTGTGGGACCTAGGGAGAGATTTTCGGTGCACTGTGGGGAAACTTTATCCATCCTGT CTTGAAGAATCTCCATGGAAGCCACCAACTTACCTTTTTTCACCAGATGGAACAGAGGAGTTTGAACTGG ATGGGGAAATGGTTGCACTTCCGCCTGTATAGGGGACTTGAGGGAGTGCCACGTGCTGAACTTGTGATAT CCTTTGCTAAACCAGAGGATAAAAAAGGAGAGTATGAGTACATAAAAATCTATCTTTGATACATTTTATT TTTATGCCCTACTTAATATTTGCATCAGTATAATATATATCAGTGTGTCATACAGAAAGATATGCTTCTG TAATATGAAATAGGTTATTTGATAACTGAGAAACTTTAAATGTATCATGTGAGCATAAGAGTCTGGATTA TCTAACATTGTTAGCCCTGTGTATGTACAGTCCAGAACTTCACTTTTTTTAGAAAATTTTTTTGTTTGTT TATCTTGTTTTTGGTTTTTTTGTTTTTTGTTTTTTTCCGAGACAGGGTTTCTTTGTATAGCCCTGGCTGC CCTGGAACTCACTTTGCAGACCAGGCTGGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGC TGGGATTAAAGGCGTGCGCCACCTCCGCCCAGCTCAGAACTTCACTTTTAAAAGTAATCTGTTCCCAGTG TGGTATCTCAAAATTGTGCTGAGATTATCTTAGTATATGTATTGGATTCCTGTACTTCCAAAGTCCTGGA ATATAGTTTTTTTAGTATAATTCCACTGCACTTAACACTAATGTTCATGTTGGTATAGAAATGTCTTTTA AATCCTATTGTTGAGCAAGATCTGCTCTCTGTCTATGGTATTACACAGGGAAAGCTGTGACTGCAGGATC AGTCTCATTCTTCTACTAGGTGCATGGACTCCCTTCCCACTGACTTACACTCGTCTATCTAGAATACAGG TTGTCCAGGCAGCTGGTCACTTACCAGGTGTGGACTTACGTTGCTGGGCTTGCAAATGAATTGCTAGTCC CTCATTGTGCGGGTCTATGTGGAGCTTTAATCAGTAAATGTCTCCACTTCCTATATTACATTAACGTTGG CTCATGCATATAACCACCTGCTGCTGATGTACTTCTCCATCTTAAAAAGATTTAGAGTGGATTAACCAGG TCATTACATCTTAATTTAATAACAAGCATTACTGTAGAGTGATTGTGTGTAGGTATCTAATAGGTGTGTT TTTTTTTAACTTGATGTGTGTATGTTTGGGGGGCTAGGATTAGGAAGGTGAACTGTGCAGGAATTGTCTG CACTGTGCAGAAGAGCAGATCCTAGTGCTGCTCTGGCATTAATCCCAGGAACCACTAGCAGTAGTGGGGC GCCACCAATGTAACATGAGCACAGGTGCCTCATGGCAAACATGGCTGGCATGTTCAACTGCATAATGCTC TGGCACTGTATTTTGAATGACATTAATTTATTAAATAAATTGTATATAATCAGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5368323 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5368323 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14627 same embryo
	EMAGE:14630 same embryo
	EMAGE:14628 same embryo
	EurExpress:euxassay_005543 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source