EMAGE mouse gene expression spatial database

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EMAGE:14667

Fam149a family with sequence similarity 149, member A ( MGI:2387177)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005535. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005535_01	euxassay_005535_02	euxassay_005535_03	euxassay_005535_04

euxassay_005535_05	euxassay_005535_06	euxassay_005535_07	euxassay_005535_08	euxassay_005535_09

euxassay_005535_10	euxassay_005535_11	euxassay_005535_12	euxassay_005535_13	euxassay_005535_14

euxassay_005535_15	euxassay_005535_16	euxassay_005535_17	euxassay_005535_18	euxassay_005535_19

euxassay_005535_20	euxassay_005535_21	euxassay_005535_22	euxassay_005535_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14667_wholemount_strong.wlz

14667_wholemount_moderate.wlz

14667_wholemount_weak.wlz

14667_wholemount_possible.wlz

14667_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14667_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

kidney calyx

strong

regional

strong expression: see section 05 06 07 08 09 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5441
Entity Detected:	Fam149a, family with sequence similarity 149, member A ( MGI:2387177)
Sequence:	sense strand is shown >T5441 CCACGCGTCCGAGAGAATCTCCAGCGAGTTTAGCCCATAATCCGTGCATCCAACACTAGTCCTCTCCTTG AGCTCTGTGCCCCTGGTCCCTTGAACATCTTACTACACTGTGGGACAGGAGGGATTTCTCTTTGCAGAGG GTAACCAGCCTCCCTCTGTCGCCTTTACATCTGTGGGTTCATTGGAGAGGGTCTGTCAGACCCATAAGGA ACTAAACGCTGAAACAACGGATTCCTGTGGGTGGAAAAAAAGACCTAGAAGCCTCGAGGAAGCTGTGTGC CAGGACCCCAGGACCCTCTGCTCGTCCCCAGAGCCATCTGATCCAGATCGCTGCAGGGGGTGGTGGATGG AGCCCTGCAGACCAGCACCTGGTGTCCTTGACCTCCCGGCGACAGTCACAGTAACCTCTCCAGGACCAAG GCATGAAGGTCGCTGTGTTGGACCTGGGGTCTCTGTTTGCCAAGATCTTCAAGCTCTCAACAGCATCCCC GGCAGTCTCCAGCCACCCTGGAGGCGCGGCGGCCACAGGATCAGTAGACTCCGGCGCCAGCACGTCGCTT AGAGAGGCTGAGACCCTGACTTTGCTCCCATCTCTGCCTCCAGACACCGCGGCCTCACGGGAGCCCTTCA CCCCGGTGCACACTCCGCTCCCTGCCTCGCACCCCAGGAGCTCCGCAGCTGCTCAAAGGGTAGAAGCAAC AGGGTGTTCCGGATCTCTGCCCAGCAGCTCAGGAGCATCCATAGCACCGCCCTTAACCCCCTACAGTGGC TCTGCCGGCTCAGTGGCTTCAGTGACTTTACCCAGCCCAGGGGTTGACTGGGCGACCCTGCCTTCTGTCA CCATCCCCTTGGGCAGCAACAGCGTCACAGCATCAAGTCCACGGAACCCTCGCCAGCTGCGAGCTCCTGG AGAGCGAGAGCCCAGTGTATGGATGGCCCCGGGTCCGGTTCCCAAAACTCTGTTTTTCACCTTGCCTGAC ATTGGCGAGGAATGGACCTCGGACAGCGACTCCCAGGATGACCCAGAAGGAAGGGGGCTCTCGGAAGGAC TCAGGAAGCAGAGCTCTGAAAAGAGCAAAGATCCTTTACCTACGAATTTTACGAGAAACGTGCAGAAAGC CATTGATAAATTCGCCAGCGAGTCCCCCTCATCATTTTCATCCAGTGGGAGCCGGACGCCCACAGAGGCC CACAACTCATGGCCTGGCTCTTCTACACAGAGCTCTACCACGGGATTGTCTACGGAGCGGAGCTCGGTTT CTTCTTGGAGAGATGATGAATTTGACAAAGTCAGTGCACAGAAAGTCCACCAGCTGTTTTGGGAGGTTGA GGAACTGCTATTTGAAGGGAAAGTGAGTCCCCAGACCCAGAATCTCCTGGCTGAATGCAGCGAATGGGCA AGAAGGTCCCTCCATCTCAGGGTGGTAGGAAGACAGCTCGTCCCACCCACCGATGAAGGCTTCCAACATT TCCAGGGGAGCCTGCCTAGTTCTGCTACTCACGAAGCTTTACCTCATGTCCCTGACCACACCAGCAGCAG CAGAGAGCTGTGTATCTCTGGCTCTCAGATAGTCCCTGAGGTACACTCAGCCTCAGCCTTGACAGACCCT GATGGCACGGAATCGGCTGACCTCACATCATGCTCATCCCTGAAAGAAGAGGTGTACCATGTGGATGGAA ACATTGAAGAATATTTTGCCTTCGACAGAAAGCAGGATGGAGATGAGCATCTTGGACAAAGCCCAGCACT CCGTGGCAGGAAGAGGCATCGCCATGGGCTTCCTCCCATTTCCCCTGATGACTGCATCAGAGATGCTGTG GCAGCAGAAGTGTTTGACCACGTGTGGACCAATGTGGTAGAAATCCTGGAAGACCTCATTAGAAAAACCT GGGAATCTGCACTCACAGGTGGGAAAAAACATAAGGAAAAACTAAAAGTAGCTGAGAACAGATCTCCACA CGTGCTCATGTCCCGTCTCAGCACCGATGTTTGCAGTGTCCCCCCATCCCGAAGCTCTGATACGCTCCAG CCATCCTTGGCTCCCCACTTTAACCCACCCCAGTTTCCTCAGCTCCATCGCTTCTCCAGCAACTTCTACA GTGACTTGAGCGGTGTGATGACGATTCAAGCCAAACCACTCCAGCAGAGACCCACTTATTCTGCAGATAG AACACAAAATGACCAAGATGACAAACTGCCTGGAGGAGGGGTCGGAGCATCTTCCCGGCACCGACTTGGC CGAATTCTAGATGCCCGAGGGCCACAGACTTCAGTCAAGAAGACACCAGTGCACAGGAGACTGCCTTCAA TTGCCTCAGACCCACAGAGGCTGAAAACCCCCACTGTCTACAGTGATGAAATTCTGAGAGGAACCAAACT GCAAACTGGCATTGACTACCTGCCTTCCCCAGCAGCAGTGCAAACCTCCCGGAGCCGGTTACCCCCAATA GGCTCTGAGACTGGGGAGCCCAACACGGCAGCCTCAGGGTCCCGCCCTGTTTCTTACAGGGGAAGGCACC CACAAAGCCGTGTGTTCAGCGCAATGCCTGACAGCATCGAACGGTCACCCCTTCGAGAAAGGACCATTGT ATTGGAACAGCTTTCAAGGCCCAGCACAACACACACATTCCGGTCAGACACACCTCGAAAAGGCTCCCTG ACACCCGTGGAGTTTGTGGCTCACACTTGGACGGGTCAAAGTATTTTGACAGGTTCACAGTATCTGCCCA AATCTTATCAGAGGGCAACCCTGACTGCAAGAAAGAGGTTCCAAGTGGCCTCGTAATGTTACTGCTTCAG AATTATGGACTTGCTAGAAGTTATGAAGCCTTGGCCACACCATCTGTCACGTGAAAAGCAGAGGAACAAG TACTAACTGGAATATATAGTCATCTCCCTGGCAATAGGAGACGGCAGAATACCTGAGAGGAACACAAGCG AATAAGTAACTTGGTTTTGCAGACTTGTAAAGCATTGTAAAGAGAATAAAAGTTGAACTCCCTAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5369152 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5369152 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14669 same embryo
	EMAGE:14668 same embryo
	EurExpress:euxassay_005535 same experiment
	MGI:4824712 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source