EMAGE mouse gene expression spatial database

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EMAGE:14668

Zfp940 zinc finger protein 940 ( MGI:2446235)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005592. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005592_01	euxassay_005592_02	euxassay_005592_03	euxassay_005592_04

euxassay_005592_05	euxassay_005592_06	euxassay_005592_07	euxassay_005592_08	euxassay_005592_09

euxassay_005592_10	euxassay_005592_11	euxassay_005592_12	euxassay_005592_13	euxassay_005592_14

euxassay_005592_15	euxassay_005592_16	euxassay_005592_17	euxassay_005592_18	euxassay_005592_19

euxassay_005592_20	euxassay_005592_21	euxassay_005592_22	euxassay_005592_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14668_wholemount_strong.wlz

14668_wholemount_moderate.wlz

14668_wholemount_weak.wlz

14668_wholemount_possible.wlz

14668_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14668_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6798
Entity Detected:	Zfp940, zinc finger protein 940 ( MGI:2446235)
Sequence:	sense strand is shown >T6798 CGCGGTGGACCCTAGGCTTGCTTTATCAGACACACCAGGAAGCCAGTTAGAAGCCGTCTGCATGCCTGCT TGCTGGCTGGGAGCGCACGAAGCTGTGGAGGAGGACAACTGAGGAGCGTGGAGCGTTCCCGAACCCCAAA GAGAATCCCAATCTTTCTCGGGCTCTACTTGGTTGATCGTGGAACCCAGAAGAGAACTACTCAACTCAAG TTTTAAGCCATGGCCCAAAGACTGATGACGTTCAGAGACGTGGCCGTAGACTTCTCTCCAGAGGAGTGGG AGTGTCTGAGCCTGGACCAGAGGAGTTTATACAGAGATGTGATGCTGGAGAGCTACAGCCACCTCGTCTC AGTGGGACTTTTTGTTTATCGGCCACATCTGTTCTCCTTGTTGGAAAAAGGACAAGACCCCTGGATGATT TTGAGGGATGAGACAAGGGGACCTTATCCAGACATAGATTTCAGTTCTCAGAACAAGAAGTTCCTGGGAA AACCTAGCACATATGAAGAATTATCTCAAAGGGAAATGAAGGAATATTTTAGAAAACGCAGTCTTGAAGA TTTCTGTTTTGGAGATGGCTGGGATGGCAAAAATCAGTTTCAAACGCATCAAAATCAGGAATATTTTAAG CAACTGGCAGTCGCCTATGAAAAAGTGCTCACTGTGAACCAGGGTATAGTTTTCAACAACCACCAGAGAC TGAATGCAGGAGAGAAAGCAACTGAAATTCAGTGTGAAAAAGCCCTTACTGTGGAATCTGACCGTATTCT TCCTCAGCTAACTCCCCCTGGTAACAAAGTCCACAAAGACAAGGAGTGTGGGGACACCTCTGCTCCTGAA TCGGAGCGCACACACTGTCAGAGAGCTCACTGTCCCCAGACCCCTCAGCAGGGGAAGGAATCTGGAGGGC CGTTTGGTTTGCCTTCAGGAATACTGGGCCTTCCGCAAGGTCACACTGGTGAGAAGTCCTATGACTGTAA GGAGTGCGGAAAGTCCTTTACTTTGAAGTCAAACTTGACGCGACATCGCAGAATTCACACCGGTGAGAGG CCGTACACGTGTCATGGATGTGGAAAGGCGTTTACACAGAAATCCCATCTCATGTCACATCAGAAAATTC ACACTGGAAAAAAAGCTTATGTCTGTGGGGTCTGTGGAAAAGCTTTTCACTGTGACTCAAGCCTGACCAG TCATCAGAATATTCATTACCATGCAGCTGATGCTGGCAAGAAAGCATATGAATGTAAAAAGTGTGGCAAA GATTTCGATTGTCACTCAGGTCTCATTGATCACCAGAAAGTCCACACTGGAGAGAAACCTTTTAAATGTA AGCATTGTGGAAGGCTTTTTAGGTTTCATTCACATCTTAGGGCGCACACACGGATTCACTCTGGCGTGAA GTCCTATGACTGTAAGGAATGTGGAAAATCCTTTAATTTGAGGTCAAACTTGATGCGACATCACAGAGTT CACACAGGTGAGAGGCCGTACACGTGTCATGGATGTGGAAAGGCGTTTTCACAGAAATCCCATCTCACGT CACATCAGAAAATTCACACCGGAGAAAAAGCTTATGTCTGTGGAGTCTGTGGAAAAGCTTTTAGTCGCGG CTCAAGCCTGACCAGTCATCAGAATATTCATTACTATGTGGTTAATGCTGGCAAGAAAGTGTATGAGTGT AAAGAATGTGGCAAGGCTTTTGGTTGTCATTCAAATCTTAGTCGCCATCAGAAAATCCATGCTGGAGAGA AACCGTTTAAATGTAGGGACTGTGAAAAGGCCTTTGGTCAAAGAGCACATCTTACCAAACATCAGAGAAT TCACACTGGTGAAGAAGGCCCTGATGAATGTCAGAGCGTGGAAAAGTTTTTCCTTGAAGACCACAACTAT GTCGACATCAGAAAATCCATACTGGTGTGAAATGATGGAATGTATGAGGAATGTAGAATGTGGCTCTTAT TCGTGGTTTTTATTGTACCCACACTGGAATATTCACATTAGTGGCAAGCCCTGAGTCCCAGGGATGTGGG AAAGTCTACATCTGGAGTGCAGGCTTAATTGTCACCAGAGCATTCATACTGAGGAAAGAAAATAGTCCAA GGAATATGGAAAGACTATTCATGGTTTAAAGCTTAAATCATCATCAGAAAATATGTACTGTGGAGGAGGC TTAGCAACCAGGCATCTCCACCATCAACCTCAGGGACCCAGGGATCACAGGATACATCATCACCAGCTGC CAGGATGCAACGCACTCTCACCACATCCTTTGCAAGCACGTGTACAGCATGCTCTTATAAAAAGCCAGTG CCTCCTTATAGAGAGAATCCATTGTGTACTGTGTGGGGTCATCACCCATCAATAAATAGCTGAAGGCCTA TTAGCTGAAATAGAAGGAAATTAGCAAGAGATAGAAAGTGGAACATCTGGTAGCAAAAGATTTGAACTTT GGGAGTTAGACAGAGGCAGGAGATTCCCTGAACTCTGGAGGAGACAGAAGCGTGAAACCGAGGATAGGCA ACTAACAACAGTTTAACAGTTAAGTAAGTGACAAGCTAGTCAAGGAGCAACCCCAAGCTTATCAAGCAGC CATTTATTCATAAGAAATTAAGTCTCGGAGTCATTGTTCTAGAAACAAAGAGGCCAGGTAGAAAAGCTCG AGGCGAGGCTGCACCTCCTCTCCCTGTTCTTTTCCCCTCCACCCTTGCCCAGAGGCAGCCTCTGTATGCT CCCTCCTCAATAAACCTCCCAGTGAGACCTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3709361 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3709361 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14667 same embryo
	EMAGE:14669 same embryo
	EurExpress:euxassay_005592 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source