EMAGE mouse gene expression spatial database

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EMAGE:14728

Rnf31 ring finger protein 31 ( MGI:1934704)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005678. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005678_01	euxassay_005678_02	euxassay_005678_03	euxassay_005678_04

euxassay_005678_05	euxassay_005678_06	euxassay_005678_07	euxassay_005678_08	euxassay_005678_09

euxassay_005678_10	euxassay_005678_11	euxassay_005678_12	euxassay_005678_13	euxassay_005678_14

euxassay_005678_15	euxassay_005678_16	euxassay_005678_17	euxassay_005678_18	euxassay_005678_19

euxassay_005678_20	euxassay_005678_21	euxassay_005678_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14728_wholemount_strong.wlz

14728_wholemount_moderate.wlz

14728_wholemount_weak.wlz

14728_wholemount_possible.wlz

14728_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14728_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8986
Entity Detected:	Rnf31, ring finger protein 31 ( MGI:1934704)
Sequence:	sense strand is shown >T8986 GCGGTGGCTTAAGTGACCCGGGCTGCTGTGGCGCCGCGCGCACCTGCGCCAGCCCCTGGAGGGCAGAGAA GCTGAGCCTGTCTCCAGTCTCAGGATGCCGGGAGACGAGGAGCGAGGCTTCCTGGCGGCCCGCGAGGAGC TGGCGAGCGCCCTGAGGTGGGATTCTGCGCAGGTTTTTCCCCTGGAGCAGCTCATGCCGCTTCTGGCCAC CTCTCTGCCACCAGCCGCCCGCTACCTGCAGCTGGACGCCGGACGCTTGGTCCGCTGCAACGCTCATGGG GAGCCTCGAAACTACCTCAACACCCTATCCACGGCCCTGAACATCCTGGAAAAATATGGTCGCAACCTCC TCAGCCCGCAGCGGCCCCGGTATTGGCGCTCAGTGAAGTTTAATAACCCCGTCTTTCGCAGCACGGTGGA TGCTGTGCAGGGTGGCCGGGATGTACTACGGTTGTATGGCTATACTGAGGAGCGCCCAGATGGATTGAGT TTCCCCGAAGGGCAGGAGGAACCAGATGAATACCAGGTTGCTGTTGTCACACTAGAAGTACTGCTGCTTC GCACCGAGCTCAGTTTGCTGTTGCAGAATACTCATCCCAGACAGAATGCACTGGACCAGCTGCTAAGAGA GAGCGTTGAAGATGGTATGCTGCAGCTTTCAGAGTTTCACCCCCTTCTGAGGGAGATTGTTCCTGGCCCC CGCCCCTCTGCCCAAGGCTCCACTCCTGGTCCCTGTTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACT GTCCAGCCTGTAACCAAGTCTCGTGCCCAGCTTGTGACATTTTGTTCCATGGGCATCCGTCCCGTGCACA TCACCTTCGCCAAGCCCTGCCTGGGTCCCACCAGACTGCCAGCCTGAGCTCTAGTTTACCTGCCTCGTCC CAACCACGGCCCCCCTCCTCCTCCTTGGCCCTGGGAGATAGCTCTCTTTCTTCCCCTGACCCTGCAAATG CCTGTCTGCCCTGGCATTGTCTTACCTGTGCCACACTAAATGAGCCTTGGGCAGTGTTCTGTGCAGTCTG TAGTCAGCCCAAAGGCTGCAAGGTGCCGGGAATAGAGGGTTCCCATGGAACCGGGGGCCTAGAACCTGAG CCTGCACGGGATCAATGGGCCTGCCAGAGCTGTACCTTTGAGAATGAGGCAGCAGCTGTGCTATGCGCCA TATGTGAGCGACCTCGGCTGGCCCAGCCTCCCAGCTTGGTGGTGGATTCCCATGATGCTGGTGTTTGCCA ACAGTCCCTTAAGCAGGAGGATCCTTTGCTCACCGCTGCCCAGCCTCAGGTGTGGTACTGTGACCATTGT ACCTTCTGCAATTCAGGCCCTGTCTGGGTGTGTGCCATGTGCAACCGAACCCGAGACCCCATCCCTACAC AGCCTGCCCTCCAGTCCTATCCCAGCTCTTTGGAAAAGGGACGCCCAAAGCCAGGGTCCTCACAACACCT TGGTTCCTCCCTGCCTGCTTCCTGTGGAGACCCAGAGAAACAACGCCAAGATAAGATGCGGAAGGAAGGT CTCCAGCTCGTGAGCATGATCCAGGAAGGAGAAACTGCGGGTGCCAGTCCAGAAGAGGTCTTCTCAGCTC TCCAATACTCAGGCACAGAGGTGCCCCTCCAGTGGTTGCGTTCAGAGCTGTCCTACGTCCTGGAGATGGT GGCTGAGCTTGCTGGACAACAGGATCCAGAGCTGGGGGCCTTTTCCTGTCAGGAAGCCCGGAAAGCCTGG CTTGATCGCCATGGCAACCTGGATGAAGCTGTAGAGGAGTGTGTGAGGGCCAGGAGGAGGAAGGTGCACG AGCTGCAGTCCCTGGGCTTTGGGCCTAAGGAAGGGTCACTACAGGCATTGTTCCAGCATGGGGGTGACGT GGCTCGGGCCCTGACTGAGTTACAGCGCCAGCGCCTGGAGCCCTTCCATCAGCGCCTATGGGACAGAGAC CCTGAACCCACTCCCTGCTGGGATGGGCTGGACAGACAGAGCTTGGTCAGACGCCTTCTGGCCGTCTACA CACTCCCCAGCTGGGGCCGAGCAGAGCTGGCGCTGGCGCTGCTGCAGGAGACACCCAGGAACTATGAGTT GTTGGACGTGGTGGAGGCTGTGAGGCACAGCCAGGACCGGGCCTTTCTGCGTCGACTGCTTGCCCAGGAA TGTGCTGTGTGCGGGTGGGCCCTTCCCCGCAACCGGATGCAGGCCCTGATCTCCTGTGAGTGCACCATAT GTCCCGAATGCTTCCGCCAACACTTCACCATTGCCCTGAAGGAGAAGCACATCACAGACATGGTGTGCCC TGCCTGTGGCCGCCCTGACCTCACTGATGACGCTCAGTTACTCAGCTACTTCTCCACCCTTGACATCCAG CTCAGAGAGAGCCTAGACCCCGATGCATATGCCCTGTTTCACAAGAAGCTGACCGAGGCTGTGCTTATGC GAGACCCCAAGTTCTTGTGGTGCGCCCAGTGTTCCTTTGGCTTCATCTATGAACGCGAACAGCTGGAGGC GACGTGTCCCCAGTGTCACCAGACCTTCTGTGTGCGCTGCAAGCGCCAGTGGGAGGAGCAGCACAGAGGA CGGAGTTGTGAGGATTTCCAGAACTGGAAACGCACCAATGACCCAGAGTACCAGGCTCAAGGCTTGGCCA TGTACCTTCAGGAAAACGGCATTGACTGTCCGAAATGCAAGTTCTCGTACGCACTGGCCCGGGGAGGCTG CATGCACTTCCACTGCACGCAGTGTCGACACCAGTTCTGCAGTGGCTGCTACAACGCCTTTTACGCCAAG AATAAATGTCCAGACCCTAACTGCAAGGTGAAAAAGTCCCTGCATGGCCACCACCCTCGAGACTGCCTCT TCTACCTACGGGACTGGACTGCTGCCCGCCTCCAGAAACTGTTGCAGGACAATAATGTCATGTTTAATAC AGAGCCTCCAGCTGGGACACGGGCAGTCCCTGGAGGGGGCTGCAGAGTGATGGAGCAGAAGGAGGTCCAT AGTGGGTTCAGGGATGAAGCTTGCGGCAAGGAAACTCCACCTGGCTATGCCGGCCTATGTCAGGCACACT ACAAAGAGTATCTCGTGAGCCTCATCAATGCCCATTCACTGGACCCAGCTACCTTGTATGAAGTGGAGGA GCTGGAGACAGCCACTATTCGCTACCTACATTTAGCTCCTCAGCCCGCGGATGGAGAGGATCTGCCTGCT TACCAGGCCCGGCTATTACAGAAGCTGAGAGAAGAGGTACCCTTGGGACAGAGTATTGCCCGCAGAAGAA AGTAGTAGCAGAGAGCCGGGTCCTGATGGGACTTCCTGACCCAGGCCTCAGCAGCAGTTCCAGCACCAAT AAAGAGGCATCTTATGGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6811051 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6811051 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14731 same embryo
	EMAGE:14727 same embryo
	EMAGE:14729 same embryo
	EMAGE:14730 same embryo
	EurExpress:euxassay_005678 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source