EMAGE mouse gene expression spatial database

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EMAGE:14730

Tubb4a tubulin, beta 4A class IVA ( MGI:107848)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005680. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005680_01	euxassay_005680_02	euxassay_005680_03	euxassay_005680_04

euxassay_005680_05	euxassay_005680_06	euxassay_005680_07	euxassay_005680_08	euxassay_005680_09

euxassay_005680_10	euxassay_005680_11	euxassay_005680_12	euxassay_005680_13	euxassay_005680_14

euxassay_005680_15	euxassay_005680_16	euxassay_005680_17	euxassay_005680_18	euxassay_005680_19

euxassay_005680_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14730_wholemount_strong.wlz

14730_wholemount_moderate.wlz

14730_wholemount_weak.wlz

14730_wholemount_possible.wlz

14730_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14730_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

facial vii ganglion

strong

homogeneous

strong expression: see section 02 03 04 05 14 15

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 03 04 11 12 13

trigeminal v ganglion

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 13 14 15 16 17

vagus x ganglion

strong

homogeneous

strong expression: see section 04 05 11 12

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 03 04 05 12 13 14

trigeminal v nerve

strong

homogeneous

strong expression: see section 13

spinal cord

strong

regional

strong expression: see section 02 03 04 05 06 07 08

cervico-thoracic ganglion

strong

regional

strong expression: see section 10 moderate expression: see section 05 06 08 09

cervical ganglion

strong

regional

strong expression: see section 11 moderate expression: see section 05 06

dorsal root ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10

neural retina

strong

regional

strong expression: see section 02 03 04 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8892
Entity Detected:	Tubb4a, tubulin, beta 4A class IVA ( MGI:107848)
Sequence:	sense strand is shown >T8892 AGCTTGACGGAGGAGATCCCTACTTTTCCGAGGCCCCTCCCTCCATCCTCCTCCATTACTCCAGTCCCTC CATCCTTCACTGCAAGCATCCTAGGCGGGCCCAGGAGGCGGAGCACGCGGGTGACCCAGCGGTGACGTGA GTACTGCTCCGCAAATATAGAGACGCCCGGGGGCGCAGAGACAGAGCTCCGCAGCCATCTCGTCCAGAGC TAGAGCCACAGCGGCCGGTCGACACCCGTCCATCAGACGCCACCAGCAGCGCCACCACCATGCGGGAAAT CGTGCACCTGCAAGCCGGTCAATGCGGTAACCAGATCGGGGCCAAGTTCTGGGAGGTCATCAGTGATGAG CACGGCATCGACCCCACTGGGACCTATCATGGGGACAGTGACCTCCAGCTGGAGAGGATCAATGTCTACT ACAATGAGGCCACAGGTGGAAACTATGTTCCCAGAGCCGTGCTGGTCGACCTGGAACCCGGCACCATGGA CTCTGTCCGCTCCGGCCCTTTTGGCCAGATCTTTCGGCCAGACAACTTTGTATTTGGTCAATCCGGAGCA GGCAACAACTGGGCCAAGGGTCACTACACCGAGGGCGCGGAGTTAGTGGATGCCGTCCTGGACGTGGTGC GCAAAGAGGCGGAAAGCTGCGACTGTCTCCAGGGCTTCCAGCTCACCCACTCGCTCGGAGGTGGCACCGG CTCAGGCATGGGGACCTTGCTCATCAGCAAGATCCGAGAGGAGTTTCCAGACAGGATCATGAATACGTTC AGCGTGGTGCCATCACCCAAGGTGTCTGACACGGTGGTGGAGCCCTACAATGCCACACTGTCTGTGCATC AGCTGGTGGAGAACACTGATGAGACCTACTGCATCGACAACGAGGCCTTGTACGACATCTGCTTCCGCAC GCTCAAGCTGACCACGCCCACCTACGGGGACCTCAACCACCTCGTGTCAGCCACCATGAGTGGAGTCACC ACCTGCCTACGTTTCCCGGGCCAGCTCAATGCAGATCTACGCAAGCTGGCTGTGAATATGGTGCCATTCC CCCGTCTCCACTTCTTCATGCCAGGATTCGCACCCTTGACCAGCAGGGGCAGCCAGCAGTACCGGGCCCT CACCGTTCCTGAGCTGACCCAACAGATGTTCGATGCTAAGAACATGATGGCTGCGTGTGACCCGAGACAC GGTCGCTACCTGACTGTGGCTGCTGTCTTCCGGGGACGGATGTCCATGAAGGAGGTAGACGAGCAGATGT TAAGTGTGCAGAGCAAGAACAGCAGTTACTTCGTTGAGTGGATCCCCAACAATGTCAAGACAGCCGTATG TGACATCCCACCCCGGGGCCTGAAGATGGCAGCCACCTTCATCGGCAACAGCACTGCCATCCAGGAGCTG TTCAAGCGCATCTCGGAGCAGTTCACCGCCATGTTCAGACGCAAGGCCTTCCTGCACTGGTACACGGGCG AAGGCATGGACGAGATGGAGTTTACGGAAGCAGAGAGCAATATGAACGACCTGGTGTCCGAGTACCAGCA GTACCAGGATGCCACTGCTGAAGAGGGCGAGTTCGAAGAGGAGGCTGAAGAGGAGGTGGCTTAAGTCTCC TGCCATCACTCTGTCCCTGGGGCCCACCAGCAAAGCTTTGACCCTAAGCATCACACCCCTGCACCTAGTT GCCTCATTCCCTAGGACCCCATGAGCATCTTCACCATGAGGGCCAAGCCCAGGTTGCTTCTATTTGCTTC ACCTTTAACTCCTAAACCCCACTGTCTCTCCAACCTGCCAGGGAAGGGCTCTTCTAGTTCCCATGAGCGC CCCTCAACACATGTACACACACACACACACTCCACCTTCTTAGATCTTGAAAATCCTTTCCTTTATGCCC TGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTGACATGCCCAGATATTTCTCTAGATCTT AACATTTTCCCCAGCCTCCAAGTCCTCTCACCTTGCCTTACCTCTGACCTCACAGCTACCCACTCCTGAC CCAGCACCTTCACTCTGGCTCCCGTAAGTCCCCGTATGTTCCTGCCCTTGTGCCTTAGTTCAGTTCCTAC CAGAGAAGTGCTGAAGGACGAGGAAAAAGAGAGGCCTGTTGCCTGGGGTGAGAGAGCCCCTCCCTAAAAT CCACCCCACCCTGCTCTCCCCTGCTGACCCTCCATCAATAAATGAGTCATTGCTTTGGAAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6415577 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6415577 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14731 same embryo
	EMAGE:14727 same embryo
	EMAGE:14729 same embryo
	EMAGE:14728 same embryo
	EurExpress:euxassay_005680 same experiment
	MGI:4829012 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source