EMAGE mouse gene expression spatial database

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EMAGE:14773

Med23 mediator complex subunit 23 ( MGI:1917458)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005743. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005743_01	euxassay_005743_02	euxassay_005743_03	euxassay_005743_04

euxassay_005743_05	euxassay_005743_06	euxassay_005743_07	euxassay_005743_08	euxassay_005743_09

euxassay_005743_10	euxassay_005743_11	euxassay_005743_12	euxassay_005743_13	euxassay_005743_14

euxassay_005743_15	euxassay_005743_16	euxassay_005743_17	euxassay_005743_18	euxassay_005743_19

euxassay_005743_20	euxassay_005743_21	euxassay_005743_22	euxassay_005743_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14773_wholemount_strong.wlz

14773_wholemount_moderate.wlz

14773_wholemount_weak.wlz

14773_wholemount_possible.wlz

14773_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14773_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

moderate

homogeneous

moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8752
Entity Detected:	Med23, mediator complex subunit 23 ( MGI:1917458)
Sequence:	sense strand is shown >T8752 GACCCTGGCTCTCACCCGTCTGTCGCCGGGTTCCCCGGGAGCCCGGGGCTCGATGGTACCGATGGAGACG CAACTGCAGAGCATTTTCGAGGAGGTGGTGAAAACAGAAATTATAGAAGAGGCCTTCCCAGGAATGTTTA TGGATACCCCCGAGGATGAAAAAACAAAACTAATTAGCTGCTTGGCGGCCTTCCGGCAGTTTTGGAGTGG ACTTTCTCAGGAGTCTCATGAACAGTGTGTTCAGTGGATTGTTAAATTTATTCATGGCCAACACAGCCCC AAAAGAATTTTTTTCCTCTATGACTGCTTAGCAATGGCAGTTGAGACTGGTCTCCTTCCACCCAGGATGG TTTGTGAATCTTTGATAAACTCTGACTCTCTTGAATGGGAAAGAACACAGCTTTGGGCCTTAACATTTAA GCTGGTTCGGAAAATCATTGGAGGAGTGGATTACAAGGGTGTTCGAGACCTTCTCAAAGCGATTTTGGAG AAAATCTTGACAATCCCAAATACAGTGAGCTCTGCTGTTGTCCAGCAGCTTCTGGCAGCAAGAGAGGTTA TAGCTTATATCTTAGAACGAAATGCCTGCCTATTGCCGGCCTACTTTGCAGTCACAGAGATCAGAAAACT ATATCCTGAGGGAAAACTTCCACACTGGTTACTAGGAAACTTGGTATCAGACTTTGTGGATACCTTCAGG CCCACAGCAAGGATTAACTCCATTTGTGGCCGATGCAGTCTTCTGCCAGTTGTGAACAACTCGGGAGCCA TCTGTAACTCATGGAAACTGGACCCTGCAACTCTGCGATTTCCTTTGAAAGGCCTTTTGCCATATGATAA GGATCTGTTTGAACCGCAGACTGCTTTGTTGAGATATGTACTGGAGCAACCTTATTCCAGGGATATGGTC TGCAATATGCTAGGTTTAAATAAGCAGCACAAGCAGCGCTGCCCTGTGCTGGAGGACCAGTTGGTGGACC TGGTGGTGTACGCCATGGAGAGGTCAGAGACCGAGGAGAAGTTTGACGATGGGGGAACCAGCCAGCTCCT GTGGCAGCACCTTTCCAGCCAGCTCATTTTCTTTGTGCTTTTCCAGTTTGCCAGTTTCCCACATATGGTC CTCTCTCTCCACCAGAAGTTAGCAGGGCGAGGACTGATTAAAGGGCGAGACCATCTGATGTGGGTGCTGC TGCAGTTCATTTCTGGAAGTATTCAGAAAAATGCCCTCGCTGACTTCCTCCCTGTCATGAAGCTCTTTGA CCTGCTGTACCCGGAGAAGGAGTGTATCCCAGTTCCTGATATTAACAAACCCCAGTCAACGCACGCCTTT GCAATGACTTGTATTTGGATTCATCTTAATAGAAAAGCTCAAAATGGCGACTCCACGCTCCAGATCCCAA TACCCCATTCCCTGAAGCTGCACCATGAGTTCCTCCAGCAGAGTCTGAGAAATAAAAGCTTACAGATGAA TGACTATAAGATTGCCCTGCTATGTAACGCGTATTCCACAAACTCAGAGTGTTTTACGTTACCTATGGGA GCTCTGGTAGAAACTATTTATGGAAATGGAATTATGAGAGTGCCTCTCCCTGGAACGAGCTGTTTGGCCT CAGCATCAGTGACTCCCTTACCGATGAACCTCCTGGATTCACTGACGGTGCATGCCAAGATGAGCCTTAT TCACAGCATTGCAACCAGAGTGATAAAACTGGCTCATACGAAATCCAGTGTTGCGCTGGCTCCAGCCCTA GTGGAAACTTACAGCCGTTTACTGGTCTACATGGAAATTGAGTCTTTGGGCATCAAAGGATTTATCAGTC AGCTCCTGCCCACTGTCTTCAAGTCCCACGCCTGGGGCATCCTGCACACACTGCTGGAGATGTTCAGCCA CCGAATGCACCACATTCAGCCCCACTACCGAGTTCAGCTCCTGAGCCATCTCCACACACTGGCTGCAGTC GCACAGACCAACCAGAACCAGCTCCATCTGTGTGTGGAGAGCACTGCACTGAGGCTCATCACAGCCCTGG GAAGCTCAGAGGTCCAGCCGCAGTTCACGCGTTTCCTCAATGATCCCAAAACAGTGCTGTCCGCCGAATC TGAAGAGCTGAACCGAGCCTTGATCCTGACCTTAGCCAGAGCAACCCACGTCACAGATTTTTTTACAGGA TCTGATTCAATACAGGGAACTTGGTGTAAAGATATCCTTCAGACCATCATGAACTTTACTCCTCATAACT GGGCTTCACATACCCTTAGCTGTTTTCCAGCCCCTCTGCAGGCTTTCTTCAAGCAAAATAACGTGCCCCA GGAAAGCCGTTTTAATCTGAAGAAGAATGTGGAAGAAGAGTACAGGAAGTGGAAGTCAATGACTGATGAA AACGAGATCATCACCCAGTTCTCTGTGCAGGGCTTCCCTCCACTCTTCCTCTGTCTCCTCTGGAAGATGC TCCTGGAGACGGATCATATTAGTCAGATCGGCTACAAGGTCCTGGAGAGGATTGGGGCCAGGGCCCTGGT GGCCCATGTGAGAACATTTGCAGATTTCCTGGTGTATGAGTTCTCTACGTCAGCAGGAGGTCAGCAACTC AATAAGTGCATTGAAATTCTCAACGACATGGTGTGGAAGTACAACATTGTCACGCTGGACAGACTCATTC TCTGCCTGGCTATGCGTAGTCATGAAGGAAATGAAGCCCAGGTTTGTTATTTCATAATTCAGTTGCTGTT GCTCAAACCAAATGACTTCAGAAACCGAGTAAGTGACTTTGTGAAGGAGAATTCCCCAGAGCACTGGCTC CAGAGTGACTGGCACACCAAGCACATGAGCTACCACAAGAAATATCCCGAGAAGCTGTATTTTGAAGGCC TGGCAGAGCAGGTTGACCCTCCTGTACCAATCCAGTCCCCTTATCTGCCCATCTACTTTGGAAATGTGTG TCTCCGGTTCCTTCCAGTATTTGATATAGTAATCCACAGGTTTTTAGAGTTGCTTCCAGTGTCCAAATCA CTAGAGACTCTCCTGGATCACCTAGGGGGCTTATATAAATTCCATGACCGGCCAGTGACTTATCTGTATA ATACTTTGCACTATTATGAAATGTGCCTGAGAAACCGTGACCATCTCAAACGAAAACTTGTCCATGCAAT CATTGGCTCCCTCAAAGATAACCGGCCACAGGGCTGGTGTCTCAGTGATACCTACCTGAAGCATGCTATG AATGCACGGGAGGACAACCCCTGGGTTCCGGAGGACTCCTACTACTGCAAGCTGATTGGCCGACTGGTAG ACACCATGGCGGGCAAGTCTCCAGGACCCTTCCCAAACTGTGACTGGAGATTCAATGAGTTTCCCAACCC CGCTGCCCACGCTCTGCATGTGACGTGTGTGGAACTCATGGCCTTGGCGGTTCCAGGCAAAGATGTGGGG AATGCCCTTCTCAATGTCGTCCTGAAAAGCCAGCCCTTAGTGCCAAGGGAGAACATTACAGCGTGGATGA ATGCAATTGGTCTGATCATCACGGCCCTCCCAGAGCCGTACTGGATTGTGCTTCATGACCGAATTGTGAA TGTGATCAGCAGTTCTAGCCTGACGTCAGAGACAGAGTGGGTCGGTTACCCGTTCCGCCTCTTCGACTTC ACCGCTTGTCACCAGTCCTACTCGGAGATGAGCTGCAGCTATACGTTAGCTCTAGCCCATGCCGTGTGGC ACCACTCCAGCATCGGGCAGCTCTCGCTTATCCCAAAGTTTCTCACTGAGGCGCTTCTCCCGGTGGTGAA GACTGAGTTCCAGTTGCTCTACGTGTACCATCTTGTGGGGCCATTTCTACAGAGGTTCCAACAGGAGAGA ACCCGGTGCATGATTGAGATTGGCGTGGCATTTTATGACATGCTGTTGAATGTAGACCAGTGCAGCACGC ACTTAAATTACATGGACCCCATCTGCGACTTCCTGTATCATATGAAGTATATGTTTACTGGTGACAGTGT GAAAGAGCAAGTAGAGAAGATTATCTGTAATTTAAAGCCGGCTTTAAAGCTTCGTCTTCGATTCATCACG CACATTAGCAAGATGGAGCCGGCAGTGCCACCCCAGGCCCTCAACAGCGGGTCTCCAGCACCTCAGTCTA ACCAGGTGCCAGCATCTCTGCCGGTGACTCAGTGAAGCCAGAGGCACTGTGACTGATGCAGAGGAGTGGA CTCACGTCTAAATCTGAACACCGTGCTAGTAAAACGAGAGCAGCCACCATGTTCAGATTGGTTTATTCTG ATTCAAATACTGAATCCCTCTCCTTGTCTATGTTTTGTCTCTAAGAGATAAGTAAGTTACATGTTGTTCA TATTCATATCACTTTATTCAAATCCCTAGGAATGTGGGCGTTTCTATACTCAAGTGGCTTTTACTAGGCA CTCTCAGACTTCCTGTCTTAGCCATTTTTAAATTATTGTAAGTAACATCTTTACATCTTGTATACCTTAC CTTGAAGATGCGACACATGTGAAATGGCCATTACAAACCCTTTTGTACATAAATGTCCTTCCAAATAGAG CCTTTTTATTAATAAAGGGCCTTGTACTTCACTTGGAACAAAGGACGTTTCATACTCTTGTCTTTGGTTT TTTTTAATTGCCTATAAAATTTTTATTGTAAATTTTAAATTTCCAAGTGGTTCCAAGTAAGGGATGCCAA GTTTCTACCAACGTAGGTTATGGGAGTTTTGCTTCGCTCTGAGCTCTCTGTATGAAATGAAACCATCAGA AAGTTAGAAATGAAATAAAGCCGTGCTCAGACGTCTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6405418 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6405418 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14776 same embryo
	EMAGE:14774 same embryo
	EMAGE:14775 same embryo
	EMAGE:14772 same embryo
	EMAGE:14771 same embryo
	EurExpress:euxassay_005743 same experiment
	MGI:4826152 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source