EMAGE mouse gene expression spatial database

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EMAGE:14776

Arl4d ADP-ribosylation factor-like 4D ( MGI:1933155)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005741. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005741_01	euxassay_005741_02	euxassay_005741_03	euxassay_005741_04

euxassay_005741_05	euxassay_005741_06	euxassay_005741_07	euxassay_005741_08	euxassay_005741_09

euxassay_005741_10	euxassay_005741_11	euxassay_005741_12	euxassay_005741_13	euxassay_005741_14

euxassay_005741_15	euxassay_005741_16	euxassay_005741_17	euxassay_005741_18	euxassay_005741_19

euxassay_005741_20	euxassay_005741_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14776_wholemount_strong.wlz

14776_wholemount_moderate.wlz

14776_wholemount_weak.wlz

14776_wholemount_possible.wlz

14776_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14776_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

moderate

regional

moderate expression: see section 09 10 12 13 17 18 weak expression: see section 11 16 19

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 16 weak expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4139
Entity Detected:	Arl4d, ADP-ribosylation factor-like 4D ( MGI:1933155)
Sequence:	sense strand is shown >T4139 GGCTGAGACGCGACGAGTCGGTGTCTGGTTGGCGTGGCTCGCGGAGAGTGCGGCTGGCTGAAGACACCCC AGCTTGTGCTACCTGCCGTTTTAAGGTCTCCTCTTCTTGGCCTCTGCTGGAGTCCCAGACCTACTCCTGA GGTCCACCCTGCACCCTAGCTCACCATGGGGAACCACTTGACTGAAATGGCCCCTACAGCCTCGTCCTTT CTGCCGCACTTCCAGGCCCTGCATGTCGTCGTCATTGGGTTGGATTCCGCGGGGAAGACTTCCCTTCTTT ACCGTCTCAAGTTCAAGGAGTTTGTCCAGAGCGTCCCCACCAAGGGCTTTAATACTGAGAAGATCCGCGT GCCCCTGGGGGGTTCCCGTGGGATCACTTTCCAAGTGTGGGATGTTGGGGGTCAGGAGAAGCTTCGGCCA CTGTGGCGCTCCTACACCCGCCGGACAGATGGACTGGTGTTTGTGGTGGACTCTGCAGAGACTGAGAGGT TAGAGGAAGCCAGAATGGAGTTACACCGGATCAGCAAGGCTTCCGACAACCAGGGGGTGCCAGTGCTGGT GCTGGCCAACAAGCAGGACCAACCGGGGGCACTGAGTGCAGCCGAGGTGGAGAAGAGGCTGGCGGTCCGA GAGCTGGCAGCTGCCACGCTCACCCATGTGCAGGGCTGCAGTGCTGTGGACGGGCTGGGCCTGCAGCCAG GCCTGGAACATTTGTATGAAATGATCCTGAAGAGGAAGAAGGCACCTCGGTCGAGCAAGAAGAGACGGTG ACCCGAAGCTGCCCCTACCCTACCTGCTAGGGGGTCAGTACACATTATAGTCAGGCAGGGCCCGTGACCT CTCAGCCAGCCTGGGTGCTGGATCTGTCCACCGCAAAGGACTGAAGGAGCATAGGTGGCCCTGTGCGCCG CAGGGGGGAGGGAAGATGAAGTGTCTTCATTGCCCTTCACCTGAAGAGGGTAGGGAGGGGCTGCAAGACC GGAGAAAGATGTAAATGCTAACTCTACCTCCTACCTCGGCCCTGGCTCTCCTGTCCTTCCCTGGCATTTT ATTTGGAGATATATTTTTATCTTTGCAGGGGGAGGGTGTGTAGAAGGTTATCCAGAGAGTGCATTTGGGG GTGAATTGAGAACATCTCCACAGTCGAGGAGAGGGGTCTCCTCAGCTGCTCTTCTCACAGCTGTTCTGTG TCTGTGAAAATGCCAAGAATTCTCCCCACAATTGTAGATCCCCGGCCCTTTTTATAAGCCTCGAGTGTCC TCTGTATTACTGTATTTTGTTTGTTTTTTGGGTTTTTTTTTTTTTTTATCATTTGCCTGTAAGTTATTAA AGCTGGATGATTCTGGTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4481779 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481779 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14774 same embryo
	EMAGE:14773 same embryo
	EMAGE:14775 same embryo
	EMAGE:14772 same embryo
	EMAGE:14771 same embryo
	EurExpress:euxassay_005741 same experiment
	MGI:4823240 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source