EMAGE mouse gene expression spatial database

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EMAGE:14780

2610008E11Rik RIKEN cDNA 2610008E11 gene ( MGI:1919378)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005740. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005740_01	euxassay_005740_02	euxassay_005740_03	euxassay_005740_04

euxassay_005740_05	euxassay_005740_06	euxassay_005740_07	euxassay_005740_08	euxassay_005740_09

euxassay_005740_10	euxassay_005740_11	euxassay_005740_12	euxassay_005740_13	euxassay_005740_14

euxassay_005740_15	euxassay_005740_16	euxassay_005740_17	euxassay_005740_18	euxassay_005740_19

euxassay_005740_20	euxassay_005740_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14780_wholemount_strong.wlz

14780_wholemount_moderate.wlz

14780_wholemount_weak.wlz

14780_wholemount_possible.wlz

14780_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14780_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9273
Entity Detected:	2610008E11Rik, RIKEN cDNA 2610008E11 gene ( MGI:1919378)
Sequence:	sense strand is shown >T9273 CAGTTCCGAAGGATCGGAAATCACTGAGGCCCCTGTCTCCTGTGACAGTCGCGGTGGGGAGGCCCGCGCA GGACGCAGGATGTCGGCAGAGCCTGTCATGGAGTCGGTGTCATTCGAGGACATTGCTGTGTTCTTCACCT GGGAGGAGTGGCAGGACCTAGACATTGCTCAGAAGTTGCTCTACAGGGATGTGATGCTGGAGAACTACAG TAGCCTGGTGTCCTTGGGCTGCTGTGTGATCAAACCTGAGTTGATCCTCATGTTGGAACGTGAATTTGGG CCATGGAGGGTAGCAGATACCTCAGTCTGGAACCTTCCAGGGTACTGCATGACCAGACCAGAATTGAACT TCAAGTTATCGCATGGATTTGAGCCGTGGAATGGCTCAGAAGCCTCGCTTTGGAGCCTCCCAGATGTCCA TAAGGTAAGTGTCCTGGGAAATCAAGAGACACACTTGTGGCAAGCTGAAGTCACTGGTGGCATTACATCC TATGAACAGATGGTTGAAGCAGAACTACAGATTCAAGAAAAAATTCATCGAGAGGCAAAAATACATGAAT ATAAAGATTGTATGGAAACATTTTATCCGAATTCACAGCACACCAGGAATCAGACATCTCAGTCATATGA GAACCCCTTTGGATGGGAGGAATTTAGAAAAGCTTTCTATTATCAATCAACCCTCACTCAACATCAAAGA TTTCATATAGGCGAAAAACTCTATGACTCTCCACAGTGCTGGGAAATTTTCCCCTGGAAGTCCCAACTCA GTGTACATGAGACATTTCATGCAGGTGAGAGACGCTATGAGTGTGAAGAATGCAGGAAGTCTTTTCATCG GAAGGCAAACCTTATTCGACATCAGAGAAGAACACATAGCAGAGAGAAGCCGTATGAATGTATAGAATGT GGGAAAACTTTCTACTGTAAGTCAGATGTCACTCGACATCAGAGAAGAACTCACAGTAGAGAAAAACCCT ACGAATGTGTAGAATGCAGTAAGACTTTCTACTGTAAGTCATACCTCATTCGACATCAGAGTAGAACTCA CAGTAGAGAGAAGCCCTATGAGTGTACAGAATGTACTAAAACTTTTTACTGTAAATCAGATCTTACTCGT CATCAAACAACTCATAGTGGGGAAAAGCCTTTTGAATGTAATGAATGCTCTAAAACATTCTACTATAAAT CAGACCTCGCTAATCATCAGAAAACGCATACAGATGATAATCCCTATGAATGTAAAGAATGTAGAAAAAC TTTCTGCTCCAAGTCAAGCCTCAATCAGCATCATAGGATTCATACAGGTGAGAAGCCCTATGAATGTAAT GAATGTAAGAAAAGTTTCAATTCTAAGTCAAATCTCACTGAGCATCAGAGAAGAACTCATACCAGAGAGA AACCCTATGAATGTAACGAATGTTGGAAATCCTTCTACTGTAGGTCAGAACTTACGAATCATCAGAGGAC TCATACAGTTGAGAGGTACTATGAATGTAAAGAGTGTAGAAAAAATTTCTACTGTAAGTCAAACCTCAAT CAACATCAAAGAACTCACACCGGAGAGAAACCGTATGAATGTAAAGACTGCAACAAAGCTTTCTATTGTA AGTCAAACCTCAATCAACATCAAAGAACTCATACAGGTGAAAAACCTTTTGCATGTAAAGACTGTAGTAA AGCTTTCTATTGTAAGTCAAGCCTTGTTAAACATCAAAAAATCCATTCAGGTGAGAAGCCATATGAATGT GAAGAATGTAGAAAAACTTTTTTCCAAAAGTCAGACCTCACTCGACATCAGAGGACACATACAGGTGAAA AGCCCTATGAGTGTAAAGACTGTAGCAAAACTTTCTATTGTAAGTCAAACCTCAATCAACATCAGAGAAC TCACACGCATGAAAAACCTTATGAATGTAAAGAATGTAGGGAAACATTCTATTCTAAGTCAGAGCTTACA GAACATCAAAGAACTCATACAGATGAAAAACCATATGTGATGTTTAATATTGATTGTCAACTCGACAAGA TCTGGGATCATCTGGGAGACAGGCCTGTAGGCATGCCTGTGAAAGATTATCTAGATTAAGTTAGCCTTTG GAGATGTATGTGATTATCATTATTTGATTAATTGAAATAGGAAGACCTATCATAAATATGTGTGGCACCA GTCCCTGGGTTGGGATTATGTACTGCAGGAAAAGGGATGGCACCATTCTGTAGACTAGAGTTCTAGACTA CATGAAAAGAAGGAAGGTAGATTATGCATGACCATTTGCTGTTTTCTGCTTCCTGTCTGGATGCCACTTG TCAAAATGCCTCAAGCTCATGCCACAAGAAGTTCTCTACCAATATACGATTAGGATTGTACATCAGGTGT GAAGTACTGTGACAGCAAAAAGTAACTAATACAGGAAATTGGTTTTGAGAAGTGAGGCCACTGCTCTGAT AAACTTGACTATGTGACTCTTAGGCCACTAGAAATGGCTTTCATGAGGAATGTAGAAGATTTGGAACTTT GGGCTAATAAAATCTTTGAATGCTATGCTACAGAGCTTAATGGGCCATTGTGGTAGAAGTTTATAAGTAC AGACTATGGAGGCCTGGCTCACAAAGCTTCAGAGGGGAACAAGCATTGTCAAGAACTGGGCTAGAGACAA TTTGTGTGACCCTTTCCTAAGGATCTGGCTTCATTCTACCTGTGATCTGAGAACTTGAATAACAATTTAA AGGAAAAGTACTCATTTATTGGGTGAAGAAAATTACCAAAAAGGTATATATTCAGGCTTGTGGCACAGCT CCTAGTAACTTCTCTTATAAAAGTTTACAATGAAAAAGTTCCATTGTGCTGAAGATAAATACAAAAATGA TTAAAACTTGGAGTCTATCAAGGATTAGAAAGCTAAAGCAAGGCAAAAGCTGCAGGCAAGGCAGGTATGG AAGAAGAGGCTTTGTTAAAAGAGAAACCACCCTGCTCTGCCCCCAGAACAATAGGAAATGTGTCTGGATA GCCCCACTGTCCCTATCTAAGGTTTCAGCTTGTAAAAAATTTGGACTTATTTGAAAGAAGAGAACCTGGA CTGAGAATATAGCTGAAGGGATTCCCTGCTCCCTGAAGACAACTCCCTAGGGGAAGTGTTTCTGTAGGGT CAGCACACTGTGGGTGATAGCTTTGTTCCAAGAAGATATATTTCTAACTAGCAACAGCTCTTGGAAGCTT TGTGGATGTGATACTAGTTTCATAGGTATGAAAGATGCAACATTGAAGGGGTCAAGTAGATTTGCTCCAA AGTGTCAGAATGCAGCTGAGACTAAACATTGTATGTCTAAACTGGAGTCTCTACCGGAAGGCTCTGAATG CCCAGTGCATGGACCTGTGAAGGTAAACTATAATTTGCCTTGGAGACTCCAGTATATAAGTGATGCCAGG AGCATGGGGTTATCCACCAAGGAAAGCTACAGGCATGGAGTGGGGCCAGTTGCGCAGCAGAGATGGAAGA GTGAGGCTTCTCAAGTACTTTAAATTCCAGGGGATTCTATTACAAGTCTCAGATGTAGTGGACATGCTCC AGTAAGATATGTTGTTTTCCCTGCTGGGTTTCTGCCCTAGTTTTTTCCTTTGGGAATGGGAATGTTTATG CTTTCATGTATTAGAACCATGCAACTTGCTTTTTGGTATTAAAGGGCCTCACAGAGTATTTCAGATGAAA CTTGGACTTTGGATATTAAAACATTGTTGGAATTCTTGAAGACTAGGGATTTTTTTGTATTGTATATGAC CCACTTTATGAAATGGCCATCAGCTTATGGGGACAAGAGAGGAAAAGTTATGACTCAACAGTGATAATCA TGTCATGTTGACAAGGAGTACAGTTATGGTGGCTAATATTGTCAGCTTGACTGAATCTGGAATCACCTGG TAGATGGACGTCTGAGAATATCTGGGGGAAATTGTCTTAAGTTAATTGGCATGGAAAGACTGACTTTAAA TGTAGTACTCCATGGGCTGAGGTCTTAGACTACATGTAAAGAAGAAAGATGACTAAGAATGAACATTCAT TGTTCTCTGTTCTTGACTGTGGATGCCTCTGGACCAGCTGCCTCAAGCTCCTGCCACTGTAACTTCCCTG TCCAGATGTATTCTATCTTTGAACTGTGAGCCAAAATAAATCCATCATTAATTTGCAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6838422 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6838422 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14777 same embryo
	EMAGE:14781 same embryo
	EMAGE:14779 same embryo
	EMAGE:14778 same embryo
	EurExpress:euxassay_005740 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source