EMAGE mouse gene expression spatial database

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EMAGE:14805

Evc2 Ellis van Creveld syndrome 2 homolog (human) ( MGI:1915775)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005744. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005744_01	euxassay_005744_02	euxassay_005744_03	euxassay_005744_04

euxassay_005744_05	euxassay_005744_06	euxassay_005744_07	euxassay_005744_08	euxassay_005744_09

euxassay_005744_10	euxassay_005744_11	euxassay_005744_12	euxassay_005744_13	euxassay_005744_14

euxassay_005744_15	euxassay_005744_16	euxassay_005744_17	euxassay_005744_18	euxassay_005744_19

euxassay_005744_20	euxassay_005744_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14805_wholemount_strong.wlz

14805_wholemount_moderate.wlz

14805_wholemount_weak.wlz

14805_wholemount_possible.wlz

14805_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14805_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7492
Entity Detected:	Evc2, Ellis van Creveld syndrome 2 homolog (human) ( MGI:1915775)
Sequence:	sense strand is shown >T7492 CCACGCGTCCGCGCGACTCTGCGGGGCCTGTGTGGGGTCTGTAGCCACCAGATGGGGGCGACGGGCCCCA CGGGCGCTGGGGGACGAGCCACGTGGGTGCTGGCTGGGAATATCCTGGCCGCTGCCCTGGTACTGGGCTC GGGTCCGCGTGCACTTCCACCATCCTTCCCTGCCCTGGGCCCAGGGTCACCATCTCGGCCCGGCCCAGCA GGACCCTGGGCAAGCTCGCAGTACAGCGATATTTCAAGAGAAGCCCGAGGTCCCTTTGAGAATGGAGTGA TATTTCAAAAATGCTCACTGGTGTCTGGGCAGAGCGAATCGCAGACCATGCACGTGCAGCTGTCCGTGAA CAACACCAGGACGCCCACATCAGTCAACCTTTCGAACCTGCTCGTGCTAGATGAGATCACTGGCCTTGCT GTGAAGGAGTCGCCTGGAAATAATACTCAGGATGGAATCCAGACTTTCAGAAAGAGCTTTCTGCAAGTGG GAGAATGCTACTCTGTCAGCTACACAGCCTCGCTGGACCCCACAGCCCTTGGGACTGGGGAGAGCCTGGA TCTTCCTGCCCGGCTCATCTTTCAGAGCCCCTCACAGAACAGAACTCAGCTAAAAGCCCCCTTTACCATC ACAGTGGAAGAAAAGATAATGGTTCTTCCTAACCATGGTCTTCACGCAGCAGGCTTCATCGCAGCCTTCC TCATCTCCCTCCTCCTGACCGTGGCAGCCCTGTTCTTCTTGGCGAGGGGTCGATGTCTGCAGGGAGGCAT GCTGTCCAGATGCCGGATTCAGCATCCTGAGAACAAGCTGGAGCCCTCGCCCTTCACCTCTGCTAACGGT GTGAGCCAGGACCTTTCCCTCAATGACCAAGTGGTTGCCATCCTGACTTCTGAGGAGCCTGGGAGCATGC TTCAAGCCCTAGAAGAGTTGGAGATCGCAACCCTGAATCAGGCAGATGCGGATCTGGAGGCTTGTCGAAA CCAGATTAGTAAGGACATCATTGCCCTTTTGATGAAGAATCTGGTCAGCGGTGGTCACCTCTCTCCGCAA ACAGAAAGGAAGATGGCCGCTGCTTTCAAAAAGCAGTTTCTGCTGTTGGAAAATGAAATACAGGAGGAGT ATGAGCGGAAGATGTTGGCACTGACGGCAGAGTGTGACCTAGAGATGAGGAAGAAGACAGAAAACCAGTA CCAGAGGGAGATGGTGGCCATGGAGGAAGCAGAAGAGGTGTTGAAGAGAGTTAGCGAGAGGTCTGCTGCC GAGTGCAGCAGCCTTTTGCGGACCCTCCATGGGCTGGAGCAGGAGGACATGCAGAGGTCCCTTACTCTGG ACCAAGCCGAGGACTTTGCTCAAGCACACAGGCAGCTGGCTGTTTTCCAGAGGAATGAATTACACAGCAT CGTCTATACCCAGATACAGAGTGCTGTCTCCAAAGGGGAACTGAGGCCTGAGGTGGCTAAAATGATGCTG CAAGACTACTCTAAAACACAGGAGAGTGTAGAAGAGCTGATGGACTTCTTCCAGGCCACCAAGCGATACC ATCTCAGCAAGAGGTTTGGCCACAGAGAGTACCTGGTCCAGAGACTGCAGGCCATGGAGACCCGGGTGCA AGGGCTGCTGAACACCGCGGCTACCCAGCTAACAAGCCTCATCCATAAACATGAGAGGGCTGGCTACTTG GATGAAGATCAGATGGAAACGCTATTGGAGCGTGCACAGACAGAAACCTTTTCTATAAAACAGAAGCTGG ACAATGACTTAAAGCAGGAAAAGAAAAGGCTCCACCAGAGATTAATAACCAGGAGGCGGCGAGAGCTTCT GCAGAAGCACAAGGAGCAGCAGAAGGAGCAGGTGTCCCTTGGCGAGGCCTCCAGCACCGCGGAGGACGCA GTCCAGTACCTGCACCAGTGGAGGAGCGTGATGGCCGAGCACACCGCTGCCCTGGAGGAGCTGCAGGAAC GTCTGGACCAGGCTGCCCTGGATGACCTCCGGGTCCTGACTGTGTCGCTGTCGGAGAAAGCCACGGAAGA GCTGAGGCGCCTGCAGAGCACGGCCATGACACAGGAGCTGCTGAAGCGCAGTGCTCCCTGGCTCTTCCTG CAGCAGATCTTAGAGGAGCACAGCCGGGAGTCTGCGGCTCGCACCACGCAACTGGAGGCAGAGGAGAGGG AACGTGGCCAGGAGCTGGTGCAGGGCGTGAGGCAGAGGCTACAGCAGGATGCACTGGAGGCCTACACGGA AGAGCAGGCAGAGCTGAGGCACTGGGAGCACTTGGTGTTCATGAAACTCTGCTGTGCGGCCATCTCATTG TCTGAAGAAGACCTGCTCCGGGTAAGGCAGGAGGCACAGGGCTGCTTCAGCCAGCTGGACCGGAGCTTGG CCCTCCCCAGAGTCCGTGCACGAGTGTTGCAGCAGCAGGCGCAGATGGCCTGGCGGGAAGCAGAGTTTCG GAAACTGGACCAGGCCCTGGCTGCGCCTGAGCTGCAGTCCAAGGCAAGAAAGCTGCGTTCCAAGGGCAGA GGCAAGGCCGATCTTCTGAAGAAGAACCTGGAGGATAAGATCCGACTATTCGAGGAGCGGGCCCCTGTAG AACTGGCTGACCAGGTGCGAGGGGAATTGCTCCAAGAGAGGGTCCAGAGGCTGGAGGCCCAGGAGGCGCA CTTTGCTGAGTCACTTGTGGCTCTGCAGTTCCAGAAGGTGGCCCGGGCTGCTGAGACCCTCTCAGTCTAC ACCGCCCTGCTCAGCATTCAAGACCTGCTGCTGGGGGAGCTGAGTGAGTCGGAGACCCTGACCAAGTCAG CCTGTGTGCAGATCCTGGAGTCCCACAGGCCGGAGCTGCAGGAGCTGCAGGAGCTGGAGAGGAAGCTGGA GGACCAGCTGGTGCAGCAGGAAGAGGCCGAGCAGCAGCGGGTCCTGGAGAGCTGGCAGCGGTGGGCGGCC GATGGACCTGGGCTGAGCGAACCTGAGGAGATGGATCCTGAAAGGCAGGTCTCTGCCATCCTGCGGCAAG CCCTGAACAAGGGCCAGAAGCTGCTGGAGCAGCATCAGCAGAGGGTGAGAGAGGAGTGGCAGAACGGTGC GGTGCTGGAAGATTCTCTGGAAAGCATTGAAGCTGACACCATGGCCAGCCTTTGCAGCCAGGGACTGAGG CTGGTGTCATACCTCTCAAGGATGACAATGGTACCAGGCAGCACACTACTGCGACTCCTGAGTGTGGTGC TTCCAGCGGCTTCACAGCCTCAGCTACTGGCCCTGCTGGACGCAGTCAGTGAGAAGCACTCCGACCACAC AGCTGAGAATGAGAGCAGTGGTGAGCAGGCTCAGGCAGAGCAGAGCAAGAGGAGGAAACACCAAGTTTGG TGGAAGGTTTTGGACAGCAGATTCAGAGCAGATCTGGTGAGTCAAGGACTGGAGAGGATGCTCTGGGCCC GCCAGAAGAAGGAGCGCATATTGAAGAAGATATATGTTCCCGTCCAGGAGAGGGTGATGTTTCCTGGGAA AGGAAGCTGGCCACACCTGTCCCTAGAACCCATTGGAGAACTGGCTCCCATCCCCATCACTGGGGCAGAC GCTATGGACATATTAAACACGGGAGAAAAGATCTTTGTATTCAGAAGCCCAAGGGAGCCCGAGATCTCTT TGCGTGTTCCTCCCAGGAGGAAAAAGAATTTCCTGAATGCCAAGAAGGCCAACAGGGCCTTGGGCTTGGA CTGACCCAGGGGGAAGCACCAGGGGCTGTCTCAAGACAGGTGCATCTTTCCTCCAGCCCACGTGGGTTGG TCTGTCCGTCTATATCATACAGCTTGCAGTATACATATGCTTGCAGCCTTGACACAGTGCCACGCAAGTT GGCCCTCCGGTGTTCAGGTCCAGTCTCTTTTGTGCCACAGGACACAAACATACCTTCCTTCTAAGTCCGT ATTACCATGATCTAGAGGGAACCCACCTTACCCCACCTTCCTCACACAGACCACTGGCTGTGGCTGGGAT GGGTCCCCACCCCACCCAGTTTATGTGAGGAAGGTGCAGCTCTCTATTCTTACAATGTGTGTTACATGCT CTGTTTTTCCATAAAGGGAACTTTAAAAATGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4237472 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4237472 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14806 same embryo
	EMAGE:14804 same embryo
	EMAGE:14802 same embryo
	EMAGE:14807 same embryo
	EMAGE:14803 same embryo
	EurExpress:euxassay_005744 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source