EMAGE mouse gene expression spatial database

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EMAGE:14807

Tmem19 transmembrane protein 19 ( MGI:1914476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005751. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005751_01	euxassay_005751_02	euxassay_005751_03	euxassay_005751_04

euxassay_005751_05	euxassay_005751_06	euxassay_005751_07	euxassay_005751_08	euxassay_005751_09

euxassay_005751_10	euxassay_005751_11	euxassay_005751_12	euxassay_005751_13	euxassay_005751_14

euxassay_005751_15	euxassay_005751_16	euxassay_005751_17	euxassay_005751_18	euxassay_005751_19

euxassay_005751_20	euxassay_005751_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14807_wholemount_strong.wlz

14807_wholemount_moderate.wlz

14807_wholemount_weak.wlz

14807_wholemount_possible.wlz

14807_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14807_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7513
Entity Detected:	Tmem19, transmembrane protein 19 ( MGI:1914476)
Sequence:	sense strand is shown >T7513 CCCACGCGTCCGGCTGCAGTCCTTCCAGGTCCCTCTAGGTGGGAGCTGCCGGCCTCCCTGCTGGCCTGGC GGGTCCTCTGTGCATCCTCCCTGCACCTTCTTTTTCCAAGGGCGGCGTTAACCTCCCCGGGTCGAGCCGG AGCGCTGCGCCTGCAGCAGTGACTAGACGTTTTGGATTTACGATTCTATTTTGTCGTTCGGTTGGTGGAA GATGTGCTCGTAGCTCTATTTTCCATGACAGATAGTGACGACACTACGTGCAAAAGGTATATAAAGATGA TTACCAATATAGTAATACTGAGCCTGATCATTTGCATTTCCCTAGCGTTCTGGATTATGTCAATGACTGC TAGCACCTATTATGGTAACTTTCGGCCTGTTTCTCCGTGGCGCTGGCTGTTCTCCGTCGTCGTTCCTGTT GTGATCGCCTGTAATGGCTTTAAAAAGAAAAGTCTAGATCACAGTGGGGCTTTAGGAGGGTTGGTGGTCG GATTCATCTTAACCATCGCAAATTTCAGCTTTTTTACTTCTTTGATGACGTTTTTCCTTTCATCTTCAAA ACTCACAAAATGGAGAGGAAACATAAAGAAGCAACTGGACTCAGAGTATAAGGAAGGAGGACAGAGGAAT TGGGTCCAGGTGTTCTGTAATGGCGCCGTGCCCACAGAGCTGGCCCTGCTCTACATGATAGAGAACGGCC CCGGGGAAATGCCCATAGATTTTTCCAAGCAACACACTGCTTCCTGGATGTGTTTGTCTCTCTTGGCTGC GCTGGCCAGCTCGGCTGGAGACACCTGGGCTTCTGAAGTTGCTCCAGTTCTGAGCAAAAGCTCACCTCGG CTAATAACAACCTGGGAGAAAGTTCCAGTTGGAACCAACGGAGGAGTCACAGCAGTGGGACTTGCCTCCA GTCTCCTCGGCGGTACCTTTGTGGGCCTGGCCTACTTCCTTACACAGTTGGTGTTTGTGAACGACTTAGA CATCTCTGCTCCGCAGTGGCCCATTATTGCATTTGGTGGTGTGGCTGGGTTATTTGGATCACTCGTGGAC TCGTTCTTAGGGGCGACGATGCAGTTCTCTGGTCTGGATGAACGCACAGGCCTGGTGGTGAGTAGCCCGA CGCAGGAGACGAAGCACATAGCGGGGAAACCGATCCTGGATAACAACGCCGTGAATCTCTTCTCCTCCGT CTTGGTGGCCCTCCTGCTCCCCACAGCCGCGTCGGGGTTTTGGCCCCGAGAGTGAACTTTCTTTTCCTTC CCAAGCAGTCGATACTGGTAAGGCCAGCCAGACTTGCAATGTGAAAGGTTTTTTTTCAGTGAATAATAAA TTCAGGGACCTTGGGAATACCAGCTCCTCCCGTGTTCCATTGTGGCGGCTGCTTTCAGCTCCCTTAGCAG CTGCCGCCTTCCTGGTGGAGCGGGAAGTGCGTACAAGTTGTCCACATTTATCCACTGTGGATGAAGCTTC ATGAACAACGGAGCCGCTCTTTCCCTCTGTGGCCGAACTGTAATATCAGATGTTCTCTGTAAAATATTAT TGTTCAGCTTCTCAAAGCAGTGTGTTGACAATTTAGAATGGTTCCCATTTGTACAGAGACTTACCGCTAT GACAGTGGCCCAAAAGTTAGACCTGCTTTAAATGACTTAACCCATACACATGGAAGCCCAAAGAAGTAAC TAACTGTCTTGAACGTCACTCATTATCTTCTTAGTCACAACTGTGAAAAATGTAGGCATTGCTTCTCCAC GCTCCTCGTCCTTTCTCCCAGTTAATGACGTCATTGTTTGGGTACATTTCTTATTCATCACGTTAGAAAA TATATGAAAAAAATATTTTTACGAAAAGAAATCATATAAATTTGCACCATTGGGATGAAGGCATAGCTCA GTGATAGCACTTGCCTCGGGTGCACGGTACCCTGGTTTAGATGCCCTGCACTGAAAGAAAATCGCCTTAC TCATTTATATCTTTGAAATACACACATATCTTTGGAATCTGGATTCCTTACTGCCGTGTGGAAGTTAATG AGTCACTGGAAAAATAATGTAATTTTTTTTTTTAAATTTACTTTCGTCAACCTTTGACCTTAATTTTGTA AGTGCCTAGAAGTGCTGTGATTTCCCAGTTGTGAGCCTTCTTGGTCCTACGTTCCTCTCCTTTGGAGCCT AAGTTGGCGGTTATATCACCAGAGGACTGCCCAGTAAGACTGTTGCCTGCCCGCTGTTAGGATTCTGTGA GACAGGGGAGGAGCAGTGTGCATAGAGCATGGACGGCTCAGCTCCTGATGGAGCCCTCAAGGGTTGGCAT CGGGGAAGCAGGGCAGCTGAAACCATTGGGAAAGCAGTTGATGGGAACAGAACAGCAAGTCTTGTTTTCA GCCTTTCTGATTTCTTTTGCTGTTTCTGGCAGCCATCTGTGTATGTAGGGTCCTGGCTAGAGAAATGGCA ACCCCCACCTCTACTGTCGTGAAAGTCTGGAGAGCTGCCATCTTCTGACCATGCAGATTCTAGGGTCTGT TCATCTGTCTTCCTTATGTTTGACTTCATGAGCGGGTGTCATCACCATGAGAATGAACTGTCCTGGCAAC CACGTCCCAGACAAATAACAACATGCCCTAAAAACCCCAAACACAGTGTGCAGCTTAATCAGCTCACCCG CTCACAGGGTATCTGGTACCTTAACTCCTGCCGGGTCTGAGTATTGTAAATACTCAAAGTAATTGTTGAA TCAGTTCGGTTGACCTGGGAGTATATTCTGGGGGAAGCGCTTAAAGATGCCAGGTGGCTACCTGAATTTC TGAGTACTGTAATGATCCCAGCATTTCTCAATTGTGTGAAACAACATTCATGAGTTTGAAACCAATGAGT CAGGTACTTACTGTAGCAGGAGTGGCAGACTGGCCAAGCAGGACCCCTACCCTGTTCTCTTTTCCAGTGC AAAAGGGCCATTCTCTTCTGTTTGCCCTAGTTGTTCTCTAAAATGTTATAAATGTGGACACTTCTATTTT AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239007 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239007 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14805 same embryo
	EMAGE:14806 same embryo
	EMAGE:14804 same embryo
	EMAGE:14802 same embryo
	EMAGE:14803 same embryo
	EurExpress:euxassay_005751 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source