EMAGE mouse gene expression spatial database

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EMAGE:14814

Ushbp1 Usher syndrome 1C binding protein 1 ( MGI:1922920)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005790. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005790_01	euxassay_005790_02	euxassay_005790_03	euxassay_005790_04

euxassay_005790_05	euxassay_005790_06	euxassay_005790_07	euxassay_005790_08	euxassay_005790_09

euxassay_005790_10	euxassay_005790_11	euxassay_005790_12	euxassay_005790_13	euxassay_005790_14

euxassay_005790_15	euxassay_005790_16	euxassay_005790_17	euxassay_005790_18	euxassay_005790_19

euxassay_005790_20	euxassay_005790_21	euxassay_005790_22	euxassay_005790_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14814_wholemount_strong.wlz

14814_wholemount_moderate.wlz

14814_wholemount_weak.wlz

14814_wholemount_possible.wlz

14814_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14814_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

moderate

single cell

moderate expression: see section 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4125
Entity Detected:	Ushbp1, Usher syndrome 1C binding protein 1 ( MGI:1922920)
Sequence:	sense strand is shown >T4125 GCCCTGGATCTCAGGCCTTTCCCAAGGTGCTCTGGAGAAGCAGAGAAGAGCGCAGCAGGGTCAACCCCCA GGGAAACAAAAAAACAAAAAACAAGGATTCTAAGGTGACGAAGGCAAGAACTGAAAGCAGACTGTAGTGA CAGAGGCAATGAGTGCCCGGGCCACACGGCCCCGAAGCCGTCGAGGGAGGCATCCTCTGCCTGGGGAGCT AGACCCTGTAGCTGAGAGCTCAGAGGAAGTAGATGCCAACAATGGATGCTTCGAGACTAAACCTGAGCTG CATCAGGAATACCTTGGTCCTGGCCTTCAGAGCCCAAGGAACTGGATCAAGGAGGGTTGTGATGTGGGAT CCCATGCGGTTCTATCCTTGAGCCCAGAAGAACAGTGCGAGCCTGAGGTGGAAGCCCACCAGGTCCTGCA AGAAGCCCCCCTGGATAGTGGCCCTGCTGAGATATCAGTACCCAGTGTGTATGAGACCTTGCAGTGCAGG CTGAGCTCCCTTGAGGCAGTTGTAGCTGCCTTGCGCCACCACTCGCTAAGCTTTCCAAAGTCAGTGGAAG CAGAAGACAGAGACCAGGGGGCTCCGGGGCCCTTTGGTGATGAGAAAGAAGACGCAGGACCTGGACAGCA GGAGGCTGCTCGCCTCATAGAGAGGAACGCCTGGCTGCGGCTAGCCCTGTGCAACCGGGAGGATGAGCTG GCATGCACTCAGGCTTCACTGCAGGACGCCCAGGCAGAGAAGGAGACTCTGCAGAGACAGGTACAGGAGC TGGAGGATTCCCTGATGCAGATGGAGGCCTCCCCGCCTACCCCCATCCTCAGAGCAGGGCGTAGAAACAG CAACTCCAGTACTTCTGGGGCAGAAAGGAGACCCTGGGTGCCTCAGGACTCTCCCATGGCTCACCCCTTT CTCCAACGCCTCCGGAGTGACTCCAGCACTCAGAGCTTTGGGTGCCTTTCCACACAGCATCCAGCCCCAG AGACGTACCTCATGGAAGACCAGATGGGGCAGCTCCAGGGGAGCATTGAGAAATTGAAGTGCTTCAACCG TCTTCTTCTGGCTGTGCTCCAGGGCTACAAGGGCCGTTGTGAGAGCCTCAGCATTAAGCTGGCCAAGCGG GAAGCCGAGGCCACAGCCCTGCGCCTGGCTCTGCAGTATAGTGAGGACTGTGAGGAGGTGTATGAGGTGC TGCTAGCTCTGAAGATGGCTGGCCTAGGAACTGGAGTTGGAACTACCAAGGGTGACCTGCAGGCTGCTGA GAAGGAGGCCTCTAGGCTGCTGGTGAAGAAGGAAGCAACCATGGAGGTAGAGACACCCCAGCCCAGCTCA GAGGGCAGTGGTGTGGACAAGCCTACACCAGAGGAACTGGCCGTTCAGCTCCATGGCTATGTCCAGCATC TCCGGGAACGCTGGGCTCTGGTGAAGATCCCCGAAGAACTTGGTCCTGTCACTGCACCCAAGGCCACTAT GCCCCATGCAGAAGCAACAGTGCAGGCCATTCTGGAGATACAACCTGGCCCCACCTTGCCCCATCTGGAG AAGTCTCAGATCCAGCAGGACCTAGCAGCTACTCGGGACAGTCTGGCTGATCTGGTGCTGAGGCTGCAGC TAACACAACGAGAGAAGCGTGGGTTGGAGCTGCGGGAGGCTGCACTGCGGGCCCAGGGCCCTGCCCACCA GCTACTCCTGCAGCAGCTTCGCTGGGAGCGGGCTCACTTTGCGGGTGATGGCAGCAGTGGAGGCAGTAGC GAGGACCCAAGCAGCGACGAGGAGGCTGGGGAGGACAGACAGCAGCACTACCAGGGCCCTCTTGCCCTCT TGGATGGTCAGATGGGCAAAGTGTGGGACTCTGAGAATGTGTCCCAGGAACTGTCAGCGTCCTTGGCCCG GGCAATAGACCTGCGGGCACAGCTGCAGTCCCTGCGGCGGCAGCTGGAACAGGTGACTCAGAAGGGCCGA ACCAGACGTGTTCAGAGTGCCGAGCTGTCCAGAGAACTGTGCAAGGCTCACAGCGCTCTGGCCTTGGCCT TCAGGGGAGCCCACCGGAAGCAGGAAGAGCAACGCCGGAAATTGGAGCAGCAGGTGGCTCGGTTGCAGGC TCAGCAGGCAGAAGAGCTGGCAGTGCTGACAGCCACCGCTAGGGCCCTGGGGAAGCCTGGGGCACCCCAG CCAGCCCAGACTTTCCTGTAGGGCCCGGACCACGTTTGCATGTGACACAGAGGTGGCTGGAGTGTCACAG TGTCCTGTGGTGGGCGGAGAGGTGGTCCAGCAACTAAGAGCAGGGTCTCCAATTGCGGGCACTTGAGTTT AATTGCCAGCCTCAGCAGCCATGGCGCCTGTGACTCCGGGTCAAGGAGATCTGGCACCCCCTTCTGGCCT CTGCAGGCTCTGTACTAATGTTCCCTCACACATGTAATTAGACAAGTAACTTACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4460920 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4460920 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14816 same embryo
	EMAGE:14817 same embryo
	EMAGE:14815 same embryo
	EMAGE:14819 same embryo
	EMAGE:14818 same embryo
	EurExpress:euxassay_005790 same experiment
	MGI:4829125 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source