EMAGE mouse gene expression spatial database

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EMAGE:14816

Acot11 acyl-CoA thioesterase 11 ( MGI:1913736)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005773_01	euxassay_005773_02

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14816_wholemount_strong.wlz

14816_wholemount_moderate.wlz

14816_wholemount_weak.wlz

14816_wholemount_possible.wlz

14816_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14816_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7551
Entity Detected:	Acot11, acyl-CoA thioesterase 11 ( MGI:1913736)
Sequence:	sense strand is shown >T7551 GGAGCCTACAGACCCCCCCACAGCCTGGCAACCCAGCAAACGGAGCAGCAATGATTCAGAATGTGGGCAA CCACTTGCGAAGGGGCTTCGCCTCTATGTTCTCTAATCGCACATCCCGGAAGTCAATCTCCCATCCGGAG TCTGGAGACCCTCCTACCATGGCAGAGGGTGAAGGATACCGGAACCCCACGGAGGTGCAGATGAGCCAGC TGGTACTGCCCTGCCACACCAACCACCGTGGGGAGCTGAGCATTGGACAGTTGCTCAAGTGGATCGACAC CACAGCCTGCCTATCAGCGGAGAGGCATGCTGGCTGTCCCTGCGTCACAGCCTCTATGGATGACATCTAC TTCGACCATACCATTAGTGTCGGCCAAGTGGTGAATATCAAGGCCAAGGTGAACCGGGCCTTCAACTCCA GCATGGAGGTGGGAATCCAGGTGGTCTCTGAGGATCTGTGCTCTGAGAAGCAGTGGAGTGTCTGCAAGGC CTTGGCCACCTTTGTGGCCCACCGGGAGCTCTCCAAGGTGAAGCTGAAGCAGGTCATCCCATTGACCGAG GAGGAGAAGACTGAACATGGGGTGGCGGCTGAGCGCCGGCGTATGCGACTGGTCTATGCAGACACCATCA AAGATCTCCTAACCCACTGTGTCATCCAGGACGATTTGGACAAGGACTGCAGCAATATGGTGCCAGCCGA GAAGACCCGAGTGGAGAGTGTGGAGCTGGTGCTGCCTCCTCACGCCAATCATCAGGGCAATACCTTCGGG GGACAGATCATGGCTTGGATGGAGAATGTGGCCACCATTGCAGCCAGCCGGCTCTGTCACGCCCACCCTA CGCTCAAGGCCATCGAGATGTTCCATTTCCGAGGCCCGTCTCAGGTGGGGGACCGTCTGGTGCTCAAGGC CATCGTGAATAACGCCTTTAAGCACAGCATGGAGGTGGGTGTGTGTGTGGAGGCGTACCGCCAGGAAGCT GAGACCCAGCGCCGGCACATCAACAGCGCCTTCATGACCTTCGTGGTCCTGGACAAAGATGACCAGCCTC AGAAGCTGCCCTGGATTCGTCCCCAGCCTGGAGAGGGTGAACGGCGATACCGAGAAGCCAGTGCCAGGAA GAAGATCCGCCTGGACAGGAAATACCTTGTGTCCTGTAAGCAGGCAGAAGTGGCCCTGTCTGTCCCCTGG GACCCTAGCAACCAGGTATACCTGAGCTACTACAACGTGTCCTCTCTGAAGACGCTCATGGCCAAGGACA ACTGGGTGCTGTCCGTGGAGATCAGCGAGGTCCGCCTGTACATCCTAGAAGAGGACTTCCTCTCCTTTCA CTTGGAGATGGTGGTAAATGTGGATGCCGCCCAGGTCTTTCAGCTGCTGTCAGACCTGCGCAGGAGACCA GAGTGGGACAAGCATTACCGGAGTGTGGAGCTGGTGCAGCAAGTGGATGAGGATGACGCCATCTACCACG TCATCAGCCCCGCCCTGAGCGGGAACACCAAGCCCCAGGACTTTGTGATCCTGGCCTCTAGGCGGAAGCC TTGTGACAATGGGGACCCCTATGTCATTGCCCTGAGGTCGGTCACGCTGCCCACGCACCATGAGACACCG GAATACCAACGTGGGGAGACTCTCTGTTCAGGCTTCTGTCTGTGGCGTGAGGGGGACCAGATGACTAAGG TTTCTTACTACAACCAGGCCACCCCCGGCTTTCTCAACTATGTGACCACCAATGTGTCCGGCCTGTCCTC AGAATTCTACAACACTTTCAAGGCTTGTGAGAGTTTTCTGTTGGACAACCGGAATGACCTAGCTCCCAGC CTCCAGACCCTCTAGACACCACCCATGCTGCCCCAGGTCTTACACAGTGCGTGGAACAAAGCAGAGACAT TTATTCACCTTGACTCCCCAGGGAAGCCTTCCACACTAGATGGTCCAATCCTACTGGATGGTCGGTTGCT GCTCACATCTGCCTGCAAGTCTTTCCAGTACTCCTGGGATATCCTGTAATAGACTCGGGTCCTGTCCACG GCCCTGGCCGCCCACAATCCAGCCCACAAATCCACATGGCTGTTCCCAGCAGTGCTGTGGTACACTGTGA CAGTGGCTCTAGGGGAGGAGGCCAGGAGCCTGGCCACAGTGTTGGCTGGACTCTGACTCAGTGGCCCAGC CTGCAGCTGGAAGGACACAGGTTGCCGGAGTCCCTGACACAGCTCCAGCATATCTGTGACCATCTGCTCC TGATAACCACTGTCCAGCATCTCTTCGGGCCAGCCCGGTGCCACGGTCACATGGGGGAAGACTTCAGCCA CAGCTGTGAGCAGCTCTCTGCCAGCTATGTGGCCAGGGACCACAAAACTACCATGTGAGACTGTGGACCC CACCCACACAGGCCAGGGCAGATGGCCAAGGGTAGAAAGGTGTGCTAACGTGGCCAGGGATGGCCGGAGA GCTTCGGGTTCCACTATGTTCACATGGATGCCCCAGCGCCTGGGGTGAACAGCCAGACGCAGCAGACACG ACTCCAGTGTCAGAGCAGGGCCACCTGGGGTGTGTAGGATGGGTACAGGGTTCCCAGCCTCAGGTTCCTG GAGGCCAATGTCCAGCAAGATCATGCCTTCTCTGTCTGGAAGAGGCAACAGTTTGGAGATCCTGTCATCA TGGGACGTCAGTGTTTTTTGTTAAAGGGGACATACATGGGGGCATGTCATGGGGATAGATCAAGAGGCAC CAGCCATTCTTGAGTTTGCCAGCTCCATCCCAGGCTCCAAGTGCTGTGACTGGTGACTCTCAGGCAGGCA TGACAAATTGCTGGCCTCCATTCTTGGCCTTCCTAATCACTCCCCACTCCTCTAGTCACAGTGTGGCTGA GGACTCAAGGCTGTGAAGACCAGGAGTTCCTGCTCTTGACATTATTCCTCCCACTTTGTATCCAGTGTGT GGCTTATTGTGTTGCCATGGTAACAGAGAATCAAGTTACCCACCCAAGCAAATGACAGACCTGTGGGTGA GGGTAGCCAGGAGCTTTAGAGCTGCAGGCAGACTCACCAGGAACTGTAATGGCTGCTCTTCTCCCACTGC CATTCACTTCCAGAACTAGCCACTCTACCTCCAGACCATCACCCTTGGGCTGCTGGAGAAGAGGGATCAG GCTGCCACCTGTGTAGTAGGTTCGCTTCCGGGTGGTGTTCACTGTGGGACCCAAAATTGCCAATGTGAAG GGACCACTCTCCACAAATTGCCTTGTGTATGCACTGGCTCTCCCGCATCCTAAGCCCTGTGGCTGTCACT TCTCCCTTTTGGGTAGATTGGAAGTCATTGTCTCATGGCCTGACATTCAACAGAATTTACTGGGCCAGAA ACCAAACAGAGCTAGAGATGAGGGGAGGGGGCTCCAAAGCTTGTCTCCTCCCTTTCCCAGGCACAACTCC CTCAATAGGAGTGGAATCTGATGTGACTCCTTGGGATGACTTAAGTGTAGATGCCCCTAGACACAGGGTC AAGAGAACAGGGCATAGTGGCATCTGGCTCATGAACTAGTGGGCACGTAAGAGGGCTGTGCTTGAGACCC TATTGCCAAAGGTGAATGGTGCTGTGGAAGCCAGGGCACTCGAGTGCGTGCATGTGTGTGTGTATGTGTG TGTATGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGTGAGACTTGGCTAGGT TGTGATCAGATTGCACTCCTACAAAATCCTTGTGGTTTTGGACTTCCATTGCCCTCTCTATCAACCAGAC TCCTGTTCTGAGGACATCGCTTGGATAACATAATATCCCAGGGGCAGAAGACAAATTCTCAGAATAGCTC CTGTCTCCTGTTCCACGCAGAATTTTCCCCATCTAGACCACCCTTTCTCCGCTTCCATGCCCACCCAAGG CTGCCCTGAGCAGAACACAGCCCACAATTCCCCTCCCAGGCTCCCTTCTTACAGGCCAGCTGCTTAAACT GCGACAAGACAGGCTCAAAGAGGTCATAGTAGATTTGGTGAGCGGCACTGTTGTCCCGGATGAAGAGGAG ATCTTCTACTGACACGGGGTCTGAGGCACCCTGCCACAGTGTTAGGCTGTACCTGGAACCAGAGAGCTGA CTCAGGGCCCGCCTGACTCTTGGCTCTACTTGTGCGCTCAGAACTGAATGCCAGCTGAGCCCAGGAGTGG AGCATCTGTTCTCTAGATTCCCACCAGAGGATGATGGAGAGCGGAGGTTCAGAGGGCAGGAGGATGTGCA CCCCTGGGATGTCTGTTTCTGAGTTACCCACCAATGGAAGCAGTGACCAGCCAGTGCCCAGAAGAGTACA AGCCAGAGGCCGAGCATAGGAGGTCGTTAGGGCAGTGGGACTGGGACAAGATGATCTTTAGGACCCCTCT TGACGTGTTCATCTTGGGGCAGAGCCCAGGATAGGTTTGGGACCAGTAGAGGGGTTTCACCTCTCAGATT GGCTCAGAAGCCAGCTGAAGTGGGGCCAAGCAGCCCGTGTCATGACAGCTCTCACTGGGAAGGTGACCTT CTGTGGCAGCGCTCCGACCAGCTCCTGCATTGTTTCCACCATGGCTTGGGTGTATGTGCTGTTTGGCAAC TGAGGCACATAAAGAGTGGTAAAGCCTGGAGAAATGGTGGCCTTTGGATATTTCTCCTGGACAAGAGTCA GAAACCTGCAGAGAGAGACCAGAGCAGAGATACCCATCTTTCCCTTCTTGTAGGCTCTTGCCACAGCGAC AGGAACAGTCAGGCCTATTGAGACTCAGCTTGGAGATTGACATCAGAGCCCTAAAGAGCAGCAGTGATTT GTACATCCTCCTGCTGATATCACCACCCTGTGCAGTCAGAGAGGTCTGGGATCCGGTCTCTACTCTGCAT CTGGTGATCCATCTGATTCTCAAAGATAGTGACTGTCAGGGCAGGGCCTTTTAAACATCCCCACATCGAC AGAGGGGCTTGAGTGAGACTTGTAAGGTGTGCAGATCTGCATGCTGACAGATGGCTTCGAGTCTCCAAAG TTTCAGATTCCTAATTTTTTTTTTTCATATGAGAAGAGCCTGTGCCACATGTCTATAATCTCAGCACTTG GGGGGTGGGAGGCAGGAGGTTCAGGGCTTCCTTCATCCCCAGATACATAGCAAGTGCAGGGCTAGCCTAG GCTACATGAAACTGCCACAAAGCAACACATTGAAAAGAGTTATTTGAATTTTGTTCTGATGCAAATTTTC AATAGGAAATTAATGTCACCCACTGCTCGGCCCCCAGATATATAACTCAGTACCCTGAGGAGAGAATCCT TGTTTGTCTTGGGCCCTAGCCTACATTTTCCAAATATAACTCATTTTGAGAATCAGTAAATGTTGGCCGG GCAGATGGAGGTGTCTGCAGAGCACAAAACTCGAGGACGGAAAGACCTGTGGGAACCTTTGCTCTGCCGT TTTGTAGACTTAGACCATTCATTTAACCTTTCAAAGATAGTTTCTATACTTACAAGATGAATATGACTAG TTGCTACCTCATTCTCTTAAGAGTTCAGCTGTATTCTGCAAATAAAATGCTTGAAACCGAAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240966 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240966 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14817 same embryo
	EMAGE:14815 same embryo
	EMAGE:14814 same embryo
	EMAGE:14819 same embryo
	EMAGE:14818 same embryo
	EurExpress:euxassay_005773 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source