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EMAGE:14978

Dock2 dedicator of cyto-kinesis 2 ( MGI:2149010)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14978 EMAGE:14978 EMAGE:14978 EMAGE:14978 EMAGE:14978
"Pseudo-wholemount" of euxassay_008013. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008013_01 euxassay_008013_02 euxassay_008013_03 euxassay_008013_04
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euxassay_008013_05 euxassay_008013_06 euxassay_008013_07 euxassay_008013_08 euxassay_008013_09
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euxassay_008013_10 euxassay_008013_11 euxassay_008013_12 euxassay_008013_13 euxassay_008013_14
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euxassay_008013_15 euxassay_008013_16 euxassay_008013_17 euxassay_008013_18 euxassay_008013_19
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euxassay_008013_20 euxassay_008013_21 euxassay_008013_22 euxassay_008013_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14978Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14978_wholemount_strong.wlz
14978_wholemount_moderate.wlz
14978_wholemount_weak.wlz
14978_wholemount_possible.wlz
14978_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14978_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36300
Entity Detected:Dock2, dedicator of cyto-kinesis 2 ( MGI:2149010)
Sequence:sense strand is shown

>T36300
GAAAAGGAGTATGGTGTCCGAGAGATGCCTGACTTCGAAGACAGGAGAGTGGGCCGCCCCAGGTCCATGC
TGCGTTCCTACAGGCAGATGTCCGTCATCTCTCTGGCCTCCATGCATTCTGACTGCAGCACTCCCAGCAA
AGTCCCCGCAGAAAGTTTTGACCTAGAGTCAGCCCCACCCAAGACTCCCAAAGTGGAGGAGGAGCCCATC
TCCCCAGGGAGCACTCTGCCAGAGGTCAAGCTGCGTCGGTCCAAGAAAAGGACCAAGAGGAGCAGCGTGG
TGTTTGCAGACGAGAAGGCAGCAACGGAGTCAGACCTGAAGCGGCTTTCTAGAAAGCAAGAGTTCATGAG
TGACACCAACCTCTCAGAGCATGCAGCCATCCCTGCCAGGGTGTCTATCCTCTCTCAGATGAGTTTTGCC
AGCCAGTCCATGCCCACCATCCCAGCCCTCACACTCTCCGTGGCAGGCGTGCCTGGGTTGGATGAGGCCA
ACACATCT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91979. Forward Primer - name:091979_F_cDNA_Dock2, sequence:GAAAAGGAGTATGGTGTCCGAG; Reverse Primer - name:091979_N_SP6_cDNA_Dock2, sequence:AGATGTGTTGGCCTCATCCAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14979 same embryo
 EMAGE:14980 same embryo
 EMAGE:14983 same embryo
 EMAGE:14982 same embryo
 EMAGE:14981 same embryo
 EurExpress:euxassay_008013 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS