EMAGE mouse gene expression spatial database

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EMAGE:14980

Doc2b double C2, beta ( MGI:1100497)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008012. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008012_01	euxassay_008012_02	euxassay_008012_03	euxassay_008012_04

euxassay_008012_05	euxassay_008012_06	euxassay_008012_07	euxassay_008012_08	euxassay_008012_09

euxassay_008012_10	euxassay_008012_11	euxassay_008012_12	euxassay_008012_13	euxassay_008012_14

euxassay_008012_15	euxassay_008012_16	euxassay_008012_17	euxassay_008012_18	euxassay_008012_19

euxassay_008012_20	euxassay_008012_21	euxassay_008012_22	euxassay_008012_23	euxassay_008012_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14980_wholemount_strong.wlz

14980_wholemount_moderate.wlz

14980_wholemount_weak.wlz

14980_wholemount_possible.wlz

14980_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14980_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

trigeminal v ganglion

moderate

regional

moderate expression: see section 09 10 16 17

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 08 13

cervical ganglion

moderate

regional

moderate expression: see section 07 08 15

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36299
Entity Detected:	Doc2b, double C2, beta ( MGI:1100497)
Sequence:	sense strand is shown >T36299 CCCTATCAAGACAGTCAGGACCAGCAGTGGAAAATAGAAAGCCTTGTTTGGGGGAAGGTGGAGGGATGGG GGTTCTGCCCTCAGGAGCTGAGAACTCTGGCCTCAGTTTCTAGGAAAGGGTAGGACACTGGTCAGTCTTG AAGCTCCCAACTGCCTCCTGCCAACTGGGATTCAGGAGCACAGGGGGAACAGGGAACCAAGGAGTTCCAC CATCATCTCAGAGCCCCATTTTCCTCAATCCATCACTGAGTGATAAGACACTGGGGTCCTGCCCTAAGCC TAGAACCCTCCTACAGCTGAAATAAGAACTCGGGGTCCCAGAACATGATTCCAATCCACAGTGCTCACCA GCCTTCCCTAGTTGGCAGGGAAAATAGGTCTCTAGCTCTCCACAGCACCAAGCCACCTGTTGCTTGTAAG AGACTGGGTTTCCCTCTTTAAAAGGTGTCGCCTGTTACTGAATTGGCAAACGCCTTCTTATTGAATCTTC ACCATCCTGTGTGGTGGGAGGCATGGTCAACCCATTTTATAAATGAGATGGCTCCAGAGTTGTTGATAAA CTTCCAGGGCCACCACGGTGTGAAGGGCTGAAGCAAAAACTCCAAGCCTTTTGACTATCTGAGGACAAGG ACTCTCAGGTCCCTCTCTCCGGAGTTAATGGGGCTTAGTGAGGTCTCTTACAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96744. Forward Primer - name:096744_F_cDNA_Doc2b, sequence:CCCTATCAAGACAGTCAGGACC; Reverse Primer - name:096744_N_SP6_cDNA_Doc2b, sequence:CTGTAAGAGACCTCACTAAGCCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14979 same embryo
	EMAGE:14978 same embryo
	EMAGE:14983 same embryo
	EMAGE:14982 same embryo
	EMAGE:14981 same embryo
	EurExpress:euxassay_008012 same experiment
	MGI:4824368 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source