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EMAGE:15150

Klb klotho beta ( MGI:1932466)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15150 EMAGE:15150 EMAGE:15150 EMAGE:15150 EMAGE:15150
"Pseudo-wholemount" of euxassay_010329. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010329_01 euxassay_010329_02 euxassay_010329_03 euxassay_010329_04
EMAGE:15150 EMAGE:15150 EMAGE:15150 EMAGE:15150 EMAGE:15150
euxassay_010329_05 euxassay_010329_06 euxassay_010329_07 euxassay_010329_08 euxassay_010329_09
EMAGE:15150 EMAGE:15150 EMAGE:15150 EMAGE:15150 EMAGE:15150
euxassay_010329_10 euxassay_010329_11 euxassay_010329_12 euxassay_010329_13 euxassay_010329_14
EMAGE:15150 EMAGE:15150 EMAGE:15150 EMAGE:15150 EMAGE:15150
euxassay_010329_15 euxassay_010329_16 euxassay_010329_17 euxassay_010329_18 euxassay_010329_19
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euxassay_010329_20 euxassay_010329_21 euxassay_010329_22 euxassay_010329_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15150Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15150_wholemount_strong.wlz
15150_wholemount_moderate.wlz
15150_wholemount_weak.wlz
15150_wholemount_possible.wlz
15150_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15150_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37572
Entity Detected:Klb, klotho beta ( MGI:1932466)
Sequence:sense strand is shown

>T37572
AGATTGCTCTACCGAGTGGAAGGGGTAAGGCTGAAAACAAGACCATCCCAGTGCACAGATTATGTGAGCA
TCAAAAAACGAGTTGAAATGTTGGCAAAAATGAAAGTCACCCACTACCAGTTTGCTCTGGACTGGACCTC
TATCCTTCCCACTGGCAATCTGTCCAAAGTTAACAGACAAGTGTTAAGGTACTATAGGTGTGTGGTGAGC
GAAGGACTGAAGCTGGGCGTCTTCCCCATGGTGACGTTGTACCACCCAACCCACTCCCATCTCGGCCTCC
CCCTGCCACTTCTGAGCAGTGGGGGGTGGCTAAACATGAACACAGCCAAGGCCTTCCAGGACTACGCTGA
GCTGTGCTTCCGGGAGTTGGGGGACTTGGTGAAGCTCTGGATCACCATCAATGAGCCTAACAGGCTGAGT
GACATGTACAACCGCACGAGTAATGACACCTACCGTGCAGCCCACAACCTGATGATCGCCCATGCCCAGG
TCTGGCACCTCTATGATAGGCAGTATAGGCCGGTCCAGCATGGGGCTGTGTCGCTGTCCTTACATTGCGA
CTGGGCAGAACCTGCCAACCCCTTTGTGGATTCACACTGGAAGGCAGCCGAGCGCTTCCTCCAGTTTGAG
ATCGCCTGGTTTGCAGATCCGCTCTTCAAGACTGGCGACTATCCATCGGTTATGAAGGAATACATCGCCT
CCAAGAACCAGCGAGGGCTGTCTAGCTCAGTCCTGCCGCGCTTCACCGCGAAGGAGAGCAGGCTGGTGAA
GGGTACCGTCGACTTCTACGCACTGAACCACTTCACTACGAGGTTCGTGATACACAAGCAGCTGAACACC
AACCGCTCAGTTGCAGACAGGGACGTCCAGTTCCTGCAGGACATCACCCGCCTAAGCTCGCCCAGCCGCC
TGGCTGTAACACCCTGGGGAGTGCGCAAGCTCCTTGCGTGGATCCGGAGGAACTACAGAGACAGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91844. Forward Primer - name:091844_F_cDNA_AV071179, sequence:AGATTGCTCTACCGAGTGGAAG; Reverse Primer - name:091844_N_SP6_cDNA_AV071179, sequence:CCCTGTCTCTGTAGTTCCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15148 same embryo
 EMAGE:15149 same embryo
 EMAGE:15146 same embryo
 EMAGE:15145 same embryo
 EMAGE:15147 same embryo
 EurExpress:euxassay_010329 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS