EMAGE mouse gene expression spatial database

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EMAGE:15154

Bves blood vessel epicardial substance ( MGI:1346013)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010325. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010325_01	euxassay_010325_02	euxassay_010325_03	euxassay_010325_04

euxassay_010325_05	euxassay_010325_06	euxassay_010325_07	euxassay_010325_08	euxassay_010325_09

euxassay_010325_10	euxassay_010325_11	euxassay_010325_12	euxassay_010325_13	euxassay_010325_14

euxassay_010325_15	euxassay_010325_16	euxassay_010325_17	euxassay_010325_18	euxassay_010325_19

euxassay_010325_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15154_wholemount_strong.wlz

15154_wholemount_moderate.wlz

15154_wholemount_weak.wlz

15154_wholemount_possible.wlz

15154_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15154_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

spleen primordium

weak

regional

weak expression: see section 01 02 03 04 05

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 12 13 weak expression: see section 04 11

left atrium

moderate

regional

moderate expression: see section 04 05 06 07 08 09 11 weak expression: see section 10

right atrium

moderate

regional

moderate expression: see section 08 09 11 12 13 14 15 16 17 18 weak expression: see section 10

heart left ventricle

moderate

regional

moderate expression: see section 07 08 09 10 11 12

heart right ventricle

moderate

regional

moderate expression: see section 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37595
Entity Detected:	Bves, blood vessel epicardial substance ( MGI:1346013)
Sequence:	sense strand is shown >T37595 GCTAATTCCCACCACTCTTCACCTCCATATGATACTGCTCAGAGTGATGTTGTCTCTAGGATGTACCCTT TATGTGGTCTGGGCCACTCTCTACCGCTGTGCCTTGGATGTGATGATCTGGAACTCGGTGTTCTTGGGTA TCAATATTTTGCATCTTTCATATCTTTTGTACAAGAAAAGACCGGTCAAAATTGAAAAGGAGCTGGGTGG TGTCTACCATCGGTTGTTTGAACCACTCCGAGTCCCTCCGGATTTGTTTAGAAGGTTAACCGGACAGTTT TGTATGATCCAGACCTTGAAAAGGGGCCAGGTTTACGCCACAGAGGACAAAACCTCAGTTGATGATCGTC TTAGTATTCTCCTAAAAGGAAGAATGAAGGTCTCCTATCGAGGACATTTTCTGCATAACATTTACCCGTG TGCCTTTATAGATTCTCCCGAATTCAGATCAACGCAGATGCACAAAGGCGAAAAATTCCAGGTCACCATT GTTGCAGATGATAACTGCAGGTTCTTATGCTGGTCAAGAGAAAGACTGACTTACTTTCTGGAGTCAGAGC CTTTTCTGTATGAAATTTTTAGGTACCTTATAGGAAAAGACATTACAAATAAGCTGTACTCACTGAATGA CCCTACTTTAAATGATAAGAAAGTTAAGAAGCTGGAGCCCCAGATGAGCCTCTGCACACAGATCTCCATG CTGGAGATGAGGAACAGTATCACCAGCTCCAGCGACGGCGAGGACGGCCTGCACCACTTTCTGCGGGGCT CCTCCAGCACGGCGTCTCTCCCCATGTCCTCCCCGCAGCAGCGAGCCTCTGCCAAGATGAAGCCAATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91719. Forward Primer - name:091719_F_cDNA_Bves, sequence:GCTAATTCCCACCACTCTTCAC; Reverse Primer - name:091719_N_SP6_cDNA_Bves, sequence:GATTGGCTTCATCTTGGCAGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15155 same embryo
	EMAGE:15151 same embryo
	EMAGE:15153 same embryo
	EMAGE:15152 same embryo
	EurExpress:euxassay_010325 same experiment
	MGI:4823525 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source